Gene-dose titration analysis in the search of trans-regulatory genes in Drosophila.

Botas, Juan; Prado, Jaime Moscoso del; Garcı́a-Bellido, Antonio

doi:10.1002/j.1460-2075.1982.tb01165.x

Cited by 122 publications

(77 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These results confirm that the vital interaction is with the T4 transcription unit. Dose-dependent interactions have been mapped to the AS-C previously (Botas 1982;Moscoso del Prado and Garcia-Bellido 1984a, b;Dambly-Chaudiere et al 1988;Garcia-Alonso and Garcia-Bellido 1988). This genetically and molecularly complex region contains four different transcription units (Tla, T3, T4, and TS) that encode related proteins (for review, see Ghysen and Dambly-Chaudiere 1988;Cline 1989).…”

Section: A Vital Interaction Between the Segmentation Gene Runt And Tmentioning

confidence: 99%

“…As sis-b, T4 interacts with the genes Sxl, da, and sis-a to initiate the female mode of development . As scute-alpha, T4 has been shown to interact with the genes h, emc, and da in the formation of the peripheral nervous system (bristles) (Botas 1982;Moscoso del Prado and Garcia-Bellido 1984a, b;DamblyChaudiere et al 1988;Garcia-Alonso and Garcia-Bellido 1988;Cubas et al 1991;Skeath and Carroll 1991).…”

Section: The Runt-t4 Interaction Involves the Sis-b Function Of T4mentioning

confidence: 99%

“…A second possibility is the use of inhibitory gene products (Parkhurst et al 1990;Erickson and Cline 1991). The bHLH protein hairy (h) and the HLH protein extramacrochaetae (emc) inhibit the function of scute-alpha (T4) and achaete (T5) of the AS-C during bristle formation (Botas et al 1982;Moscoso del Prado and Garcia-Bellido 1984a, b;Garcia-Alonso and Garcia-Bellido 1988;Rushlow et al 1989;Ellis et al 1990;Garrell and Modolell 1990;Cubas et al 1991;Skeath and Carroll 1991). Consistent with a possible inhibitory role, emc and h are expressed at the time of Sxl initiation; the embryonic Sxl RNAs are detected from -2--4 hr of development and emc and h expression are also detected at this time (Ingham et al 1985;Salz et al 1989;Ellis et al 1990;Gartell and Modolell 1990).…”

mentioning

confidence: 99%

See 2 more Smart Citations

The Drosophila segmentation gene runt acts as a position-specific numerator element necessary for the uniform expression of the sex-determining gene Sex-lethal.

Duffy¹,

Gergen²

1991

Genes & Development

130

View full text Add to dashboard Cite

Female development in Drosophila is established through the activation of the X : A target gene Sex-lethal (Sx/) by an X : A ratio of 1. X-linked zygotic genes, termed numerator elements, comprise part of the X : A ratio and are primarily responsible for the activation of Sxl in females. We demonstrate that the X-linked segmentation gene runt is required for this process and has genetic and molecular properties of a numerator element. Genetically, runt has vital dose-dependent interactions with components of the X : A ratio and alterations in runt activity alter the sexual phenotype of triploid intersexes. Molecularly, loss of runt activity results in a failure to activate appropriately Sx/in the central region of female embryos. We also show that Sx/ activation is influenced by the maternal anterior and terminal pattern-forming genes, bicoid (bccO and torso (tot). These results indicate that the "uniform" activation of Sx/requires input from nonuniformly distributed products. We have demonstrated that runt is one such product and suggest that other genes with nonuniform input exist, runt is distinguished from previously identified regulators of Sxl by its nonuniform role and by the absence of an identifiable helix-loop-helix (HLH) motif, indicating that the activation of Sx/is not controlled solely by HLH proteins.

show abstract

Section: A Vital Interaction Between the Segmentation Gene Runt And Tmentioning

confidence: 99%

Section: The Runt-t4 Interaction Involves the Sis-b Function Of T4mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

The Drosophila segmentation gene runt acts as a position-specific numerator element necessary for the uniform expression of the sex-determining gene Sex-lethal.

Duffy¹,

Gergen²

1991

Genes & Development

130

View full text Add to dashboard Cite

show abstract

“…Thus, the pattern of ac-sc expression is one of the determinants for SMC positioning. emc, genetically described as an AS-C repressor (Botas et al, 1982; Moscoso del Prado and Garcia-Bellido, 1984a), encodes an HLH protein that in vitro behaves as a competitive inhibitor of the formation of DNA-binding DA-AC and DA-SC complexes (Van Doren et al, 1991). Assuming a similar action in vivo, EMC should reduce the proneural activity of DA, AC and SC.…”

Section: Discussionmentioning

confidence: 99%

“…A candidate for modulating the responsiveness of cells to proneural function is the extramacrochaetae (emc) gene (Botas et al, 1982), a negative trans-regulator of ac and sc (Moscoso del Prado and Garcia-Bellido, 1984a Ellis et al, 1990;Garrell and Modolell, 1990; for a review see Garrell and Campuzano, 1991 (Cubas et al, 1991;Martinez and Modolell, 1991;Skeath and Carroll, 1991). However, these effects of emc appeared to have limited topographical relevance in view of the reported ubiquitous presence of the emc RNA (Ellis et al, 1990;Garrell and Modolell, 1990) The distribution of the emc RNA was determined in early, mid and late third instar larvae wing discs by in situ hybridization with a digoxigenin labelled emc probe (Figures lA-C).…”

Section: Introductionmentioning

confidence: 99%

The extramacrochaetae gene provides information for sensory organ patterning

1992

Trends in Genetics

View full text Add to dashboard Cite

The Drosophila adult epidermis displays a stereotyped pattern of bristles and other types of sensory organs (SOs). Its generation requires the proneural achaete (ac) and scute (sc) genes. In the imaginal wing disc, the anlage for most of the thoracic and wing epidermis, their products accumulate in groups of cells, the proneural clusters, whose distribution prefigures the adult pattern of SOs. These proteins then induce the emergence of SO mother cells (SMCs). Here, we show that the extramacrochaetae (emc) gene, an antagonist of the proneural function, is another agent that contributes to SO positioning. In the wing disc, emc is expressed in a complex and evolving pattern. SMCs appear not only within proneural clusters but also within minima of emc expression. When one of these spatial restrictions is eliminated, by ubiquitously expressing ac-sc, SMCs still emerge within minima of emc. When in addition, the other spatial restriction is reduced by decreasing emc expression, many ectopic SMCs emerge in a relatively even spaced and less constant pattern. Thus, the heterogeneous distribution of the emc product is one of the elements that define the positions where SMCs arise. emc probably refines SMC (and SO) positioning by reducing both the size of proneural clusters and the number of cells within clusters that can become SMCs.

show abstract

Identification of a novel mouse Iroquois homeobox gene,Irx5, and chromosomal localisation of all members of the mouse Iroquois gene family

Bosse¹,

Stoykova²,

Nieselt³

et al. 2000

Dev. Dyn.

View full text Add to dashboard Cite

Gene-dose titration analysis in the search of trans-regulatory genes in Drosophila.

Cited by 122 publications

References 10 publications

The Drosophila segmentation gene runt acts as a position-specific numerator element necessary for the uniform expression of the sex-determining gene Sex-lethal.

The Drosophila segmentation gene runt acts as a position-specific numerator element necessary for the uniform expression of the sex-determining gene Sex-lethal.

The extramacrochaetae gene provides information for sensory organ patterning

Identification of a novel mouse Iroquois homeobox gene,Irx5, and chromosomal localisation of all members of the mouse Iroquois gene family

Contact Info

Product

Resources

About