Identification of a novel mouse Iroquois homeobox gene,Irx5, and chromosomal localisation of all members of the mouse Iroquois gene family

Bosse, Antje; Stoykova, Anastassia; Nieselt, Kay; Chowdhury, Kamal; Copeland, Neal G.; Jenkins, Nancy A.; Gruß, Peter

doi:10.1002/(sici)1097-0177(200005)218:1<160::aid-dvdy14>3.0.co;2-2

Cited by 62 publications

(50 citation statements)

References 56 publications

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“…3Z) and irx5a (data not shown) are expressed in the dorsal half of the neural tube, while laterally they are expressed along the entire D/V axis (Fig. 3Z, Bosse et al, 2000;Bosse et al, 1997;Tan et al, 1999;Wang et al, 2001). …”

Section: The Class I Gene Transcriptional Response To Hh Diverges Slimentioning

confidence: 88%

Cloning of zebrafish nkx6.2 and a comprehensive analysis of the conserved transcriptional response to Hedgehog/Gli signaling in the zebrafish neural tube

Guner

Karlstrom

2007

Gene Expression Patterns

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Sonic Hedgehog (Shh) signaling helps pattern the vertebrate neural tube, in part by regulating the dorsal/ventral expression of a number of homeodomain containing transcription factors. These Hh responsive genes have been divided into two classes, with Class II genes being activated by Hh signaling and Class I genes being repressed by Hh signaling. While the transcriptional response to varying Hh levels is well defined in chick and mouse, it is only partially described in zebrafish, despite the fact that zebrafish has emerged as a powerful genetic system for the study of neural patterning. To better characterize the Hh response in the zebrafish neural tube, we cloned the zebrafish Class II Hh target genes nkx2.9 and nkx6.2. We then analyzed the expression of a number of Class I and Class II Hh responsive genes in wild type, Hh mutant, and Hh over-expressing zebrafish embryos. We show that expression of Class I and Class II genes is highly conserved in the vertebrate neural tube. Further, ventral-most Class II gene expression was completely lost in all Hh pathway mutants analyzed, indicating high levels of Hh signaling are blocked in all of these mutants. In contrast, more dorsally expressed genes were variably affected in different Hh pathway mutants, indicating mid-levels of Hh signaling are differentially affected. This comprehensive expression study provides an important tool for the characterization of Hh signaling in zebrafish and provides a sensitive assay for determining the degree to which newly identified zebrafish mutants affect Hh signaling. dbx1a; dbx2; hlx1; hlxb9; irx1a; irx3a; irx5; nkx2.2a; nkx2.9; nkx6.1; nkx6.2; pax3; pax6a; pax7; chameleon; detour; slow muscle omitted; you-too; cyclopamine; floorplate; motor neurons Keywords Results and DiscussionSecreted proteins of the Hedgehog (Hh) family are well known for their role in patterning the vertebrate and invertebrate embryo (Ingham and McMahon, 2001). During development, Hh functions in a concentration dependent manner to influence cell differentiation (Ericson et al., 1997a). Hh can also act as a survival factor (Miao et al., 1997) or a mitogen (Dahmane et al., 2001) to help shape the embryo. The morphogen activity of Hh has been well studied in the neural tube of chick and mouse (Ericson et al., 1995;Wijgerde et al., 2002), where Hh secreted from the notochord induces floorplate and ventral neural fates based on the concentration of Hh seen by a neural progenitor cell (Echelard et al., 1993). Cells respond to Hh through a * Corresponding author (e-mail: Karlstrom@bio.umass.edu) Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers t...

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Section: The Class I Gene Transcriptional Response To Hh Diverges Slimentioning

confidence: 88%

Cloning of zebrafish nkx6.2 and a comprehensive analysis of the conserved transcriptional response to Hedgehog/Gli signaling in the zebrafish neural tube

Guner

Karlstrom

2007

Gene Expression Patterns

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“…Digoxigenin-labeled probes were transcribed from linearized plasmids encoding the following mouse cDNAs: Irx1 (GenBank accession no. BU703212), Irx2 (BI111057), Irx3 (BI525434), Irx4 (BE367799), Irx5 (Bosse et al 2000), and Irx6 (Peters et al 2000). For probe synthesis, the mouse Brn1 cDNA (Hara et al 1992) was subcloned by PCR into pGEM-TEasy (Promega).…”

Section: In Situ Hybridization Of Mouse Kidneys and Kidney Sectionsmentioning

confidence: 99%

The prepattern transcription factor Irx3 directs nephron segment identity

Reggiani

Raciti²,

Airik³

et al. 2007

Genes Dev.

104

169

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The nephron, the basic structural and functional unit of the vertebrate kidney, is organized into discrete segments, which are composed of distinct renal epithelial cell types. Each cell type carries out highly specific physiological functions to regulate fluid balance, osmolarity, and metabolic waste excretion. To date, the genetic basis of regionalization of the nephron has remained largely unknown.

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“…The Iroquois (IRX) family of homeodomain genes is a subclass of the three amino acid loop extension (TALE) superfamily (Burglin, 1997;Bosse, 2000). IRX proteins act as either transcriptional repressors or activators; this difference might be regulated by alternative splicing (in this study, two splice variants of the same gene, IRX1b, were isolated).…”

Section: Expression Analysismentioning

confidence: 99%

Zebrafish IRX1b in the embryonic cardiac ventricle

Joseph

2004

Developmental Dynamics

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The synchronous contraction of the vertebrate heart requires a conduction system. While coordinated contraction of the cardiac chambers is observed in zebrafish larvae, no histological evidence yet has been found for the existence of a cardiac conduction system in this tractable teleost. The homeodomain transcription factor gene IRX1 has been shown in the mouse embryo to be a marker of cells that give rise to the distinctive cardiac ventricular conduction system. Here, I demonstrate that zebrafish IRX1b is expressed in a restricted subset of ventricular myocytes within the embryonic zebrafish heart. IRX1b expression occurs as the electrical maturation of the heart is taking place, in a location analogous to the initial expression domain of mouse IRX1. The gene expression pattern of IRX1b is altered in silent heart genetic mutant embryos and in embryos treated with the endothelin receptor antagonist bosentan. Furthermore, injection of a morpholino oligonucleotide targeted to block IRX1b translation slows the heart rate. Developmental Dynamics 231:720 -726, 2004.

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Identification of a novel mouse Iroquois homeobox gene,Irx5, and chromosomal localisation of all members of the mouse Iroquois gene family

Cited by 62 publications

References 56 publications

Cloning of zebrafish nkx6.2 and a comprehensive analysis of the conserved transcriptional response to Hedgehog/Gli signaling in the zebrafish neural tube

Cloning of zebrafish nkx6.2 and a comprehensive analysis of the conserved transcriptional response to Hedgehog/Gli signaling in the zebrafish neural tube

The prepattern transcription factor Irx3 directs nephron segment identity

Zebrafish IRX1b in the embryonic cardiac ventricle

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