Gene-dosage mapping of 30 DNA markers on chromosome 21

Delabar, Jean–Maurice; Chettouh, Zoubida; Rahmani, Zohra; Theophile, Didier; Blouin, Jean‐Louis; Bono, Rosa; Kraus, Jan P.; Barton, Jeffrey W.; Patterson, David; Sinet, Pierre-Marie

doi:10.1016/0888-7543(92)90177-t

Cited by 8 publications

(1 citation statement)

References 12 publications

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“…(ISCN 1995) and mouse homologies based on in situ hybridization data. Additional references: PIT1 (Bodner et al, 1988;Camper et al, 1990); PCCB (Lamhonwah et al, 1986); TF (Huerre et al, 1984); UMPS ; SST (Thue et al, 1995); COL6A1 (Kumlein et al, 1994;Schmutz et al, 1994); SOD1 (Delabar et al, 1992;Schmutz et al, 1996).…”

Section: Resultsmentioning

confidence: 99%

In situ hybridization of five loci to cattle chromosome 1

Schmutz

Moker²,

Berryere³

1998

Cytogenet Genome Res

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The genes for pituitary-specific transcription factor (PIT1), propionyl coenzyme A carboxylase, β-polypeptide (PCCB), transferrin (TF), trichohyalin (THH), and involucrin (IVL) were mapped to cattle chromosome 1 (BTA 1) by isotopic in situ hybridization. Two of the loci were mapped from cattle PCR products and three from human ATCC probes. PIT1 localized to segment 1q2; PCCB to 1q3; and TF, THH, and IVL to 1q4. These localizations agree with the homology previously shown between BTA 1 and human chromosome 3 (HSA 3). Some homology with HSA 1 has been established with the mapping of THH and IVL to BTA 1q4.

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Section: Resultsmentioning

confidence: 99%

In situ hybridization of five loci to cattle chromosome 1

Schmutz

Moker²,

Berryere³

1998

Cytogenet Genome Res

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Partial physical map of human chromosome 21 from fibroblast and lymphocyte DNA

et al. 1993

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A partial physical map of the human chromosome 21 including 26 genes and anonymous sequences was established by pulsed-field gel electrophoresis analysis of restriction fragments obtained from lymphocyte and fibroblast DNAs. The sizes of the restriction fragments obtained by total digestion with eight different enzymes were compared in these two tissues. Differences resulting from the variations in the methylation state of the restriction sites were frequently observed. These differences and partial digestions were used to estimate the order and the distances between genes and sequences. Six linkage groups were defined: D21S13-D21S16, D21S1-D21S11, D21S65-D21S17, (D21S55,ERG)-ETS2, BCEI-D21S19-D21S42-D21S113-CBS-CRYA1, and COL6A2-S100B. For six intergenic distances the resolution of previous maps was significantly increased.

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Gene mapping from a bovine 1;29 DNA library prepared with chromosome microdissection

et al. 1994

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Bovine gene mapping is progressing rapidly using syntenic group mapping based on somatic cell hybrids and linkage, and to a lesser extent on in situ hybridization. Single chromosome DNA libraries are a logical next step, and this was, therefore, the aim of our laboratory. Since we have access to several cattle with t(1;29) and this chromosome is readily distinguishable, we chose this as our first target--recognizing that we would not produce a "single" chromosome library in the strict sense because two autosomes are represented. We utilized an inverted microscope and a micromanipulator fitted with glass instruments pulled specifically to dissect off approximately 100 t(1;29) chromosomes per microdrop. A glass chamber made to accommodate a hanging drop was used to extract the DNA under a dissecting microscope. The DNA was then cleaved with EcoRI and inserted in lambda gtwes arms. Host cells were then infected with these phage and positive clones obtained. The first clone, isolated from this library by hybridization with a human collagen 6A1 cDNA, was mapped by in situ hybridization to bovine Chromosome some (Chr) 1q12-q14, near the centromere. The second clone, an anonymous DNA fragment (D1S11), was mapped to 1q43-q46, near the terminal end.

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Gene-dosage mapping of 30 DNA markers on chromosome 21

Cited by 8 publications

References 12 publications

In situ hybridization of five loci to cattle chromosome 1

In situ hybridization of five loci to cattle chromosome 1

Partial physical map of human chromosome 21 from fibroblast and lymphocyte DNA

Gene mapping from a bovine 1;29 DNA library prepared with chromosome microdissection

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