Gemcitabine impacts differentially on bladder and kidney cancer cells: distinct modulations in the expression patterns of apoptosis-related microRNAs and BCL2 family genes

Papadopoulos, Emmanuel I.; Yousef, George M.; Scorilas, Andreas

doi:10.1007/s13277-014-2190-8

Cited by 12 publications

(5 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Gemcitabine plus cisplatin has been researched in advanced urothelial cancer (45); however, no study has explored the efficacy and safety of this regimen in ccRCC. One study showed that the responses of kidney cancer cells to gemcitabine differed (46). By selecting therapeutic regimens based on patient risk score, we can overcome drug resistance and ensure efficacy.…”

Section: Discussionmentioning

confidence: 99%

Immune-related long non-coding RNAs can serve as prognostic biomarkers for clear cell renal cell carcinoma

Li¹,

Lu²,

Fang³

et al. 2021

Transl Androl Urol

View full text Add to dashboard Cite

Background:The immune microenvironment is a critical regulator of clear cell renal cell carcinoma (ccRCC) progression. However, the underlying mechanisms the regulatory role of immune-related long non-coding RNAs (irlncRNAs) in the ccRCC tumor microenvironment (TME) are still obscure. Herein, we investigated prognostics role of irlncRNAs for ccRCC. Methods:The raw data of patients with ccRCC were downloaded from The Cancer Genome Atlas (TCGA) database, and immune-related genes were obtained from the ImmPort database. First, we investigated the correlation between the immune-related genes and irlncRNAs. Then, we identified the differentially expressed irlncRNA pairs (ILRPs) between normal and cancer tissue samples, and prognostic model was constructed with the differentially expressed ILRPs. We further explored whether the signature risk scores of ILRPs had a considerable impact on immune cell infiltration. Finally, we performed a drug sensitivity analysis based on risk score.Results: There were 13 upregulated and 40 downregulated irlncRNAs between the ccRCC and normal tissue samples. We further selected the irlncRNAs that significantly affect the prognosis of patients with ccRCC via univariate Cox, lasso regression, and multivariate regression analyses. Twelve ILRPs were used to construct a prognostic signature. The model showed the ILRPs model could be used to assess the prognosis of ccRCC patients. Study of the influence of risk score and clinical characteristics on the prognosis of patients with ccRCC showed risk score to be an independent factor affecting the outcome of ccRCC.We further performed the difference analysis of immune cell abundance between ccRCC and normal tissue samples. The results showed that patients with higher abundance of M0 macrophages, plasma cells, follicular helper T cells, and regulatory T cells (Tregs) had a poor outcome. Finally, we performed a drug sensitivity analysis based on risk score. The results showed that high-risk score patients are sensitive to orafenib, sunitinib, temsirolimus, cisplatin, and gemcitabine. Conclusions:Our study has developed a novel and reasonable ILPRs model for prognostic prediction, which does not require transcriptional levels to be detected.

show abstract

Section: Discussionmentioning

confidence: 99%

Immune-related long non-coding RNAs can serve as prognostic biomarkers for clear cell renal cell carcinoma

Li¹,

Lu²,

Fang³

et al. 2021

Transl Androl Urol

View full text Add to dashboard Cite

show abstract

“…A recent study demonstrated that the main function of AKT is the direct inhibition of cell apoptosis (27). The Bcl-2 family is also closely associated with cell apoptosis, numerous members of which, including Bcl-2-associated death promoter (Bad), exert proapoptotic effects.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‑30a‑5p suppresses tumor cell proliferation of human renal cancer via the MTDH/PTEN/AKT pathway

et al. 2017

Int J Mol Med

View full text Add to dashboard Cite

The present study aimed to explore the effects of microRNA (miRNA)‑30a‑5p on tumor proliferation and to seek a potential therapeutic target for the treatment of human renal cancer. The results demonstrated that the expression levels of miRNA‑30a‑5p were reduced in tumor samples from patients with renal cancer compared with in normal tissue samples. Overall survival and disease‑free survival were increased in patients with renal cancer and high miRNA‑30a‑5p expression compared with in those with low miRNA‑30a‑5p. Furthermore, overexpression of miRNA‑30a‑5p suppressed cell proliferation, induced apoptosis, and promoted caspase‑3/9 activities and B‑cell lymphoma 2‑associated X protein (Bax) protein expression in Caki‑2 cells. In addition, the results confirmed that overexpression of miRNA‑30a‑5p inhibited metadherin (MTDH), upregulated phosphatase and tensin homolog (PTEN) and suppressed phosphorylated (p)‑protein kinase B (AKT) protein expression levels in Caki‑2 cells. Furthermore, transfection with small interfering RNA‑MTDH, increased the effects of miRNA‑30a‑5p on the inhibition of cell proliferation, and promotion of apoptosis, caspase‑3/9 activities and Bax protein expression levels in Caki‑2 cells. Knockdown of MTDH expression also upregulated PTEN and suppressed p‑AKT protein expression in Caki‑2 cells. In conclusion, the present study is the first, to the best of our knowledge, to provide evidence suggesting that miRNA‑30a‑5p suppresses tumor human renal cancer cell proliferation via the MTDH/PTEN/AKT pathway.

show abstract

“…Many studies have investigated the roles of miRNAs in cancer, inflammation, and immunity [ 3 4 5 ]. Recent studies demonstrated that miRNA profiles can be determined from blood [ 6 7 8 ], including circulating miRNAs and those associated with cancer or damaged tissues [ 9 10 ]. Circulating miRNAs are useful biomarkers for human diseases because they are present in a remarkably stable form in human blood, and the results may have a high degree of reproducibility within individuals [ 1 8 11 12 ].…”

Section: Introductionmentioning

confidence: 99%

Circulating microRNA expression profiling in young obese Korean women

Choi

Ahn

et al. 2020

Nutr Res Pract

View full text Add to dashboard Cite

BACKGROUND/OBJECTIVES This study investigates correlations between circulating microRNAs (miRNAs) and obesity-related parameters among young women (aged 20–30 years old) in Korea. SUBJECTS/METHODS We analyzed TaqMan low density arrays (TLDAs) of circulating miRNAs in 9 lean (body mass index [BMI] < 25 kg/m 2 ) and 15 obese (BMI > 25 kg/m 2 ) women. We also performed gene ontology (GO) analyses of the biological functions of predicted miRNA target genes, and clustered the results using the database for annotation, visualization and integrated discovery. RESULTS The TLDA cards contain 754 human miRNAs; of these, the levels of 8 circulating miRNAs significantly declined (> 2-fold) in obese subjects compared with those in lean subjects, including miR-1227, miR-144-5p, miR-192, miR-320, miR-320b, miR-484, miR-324-3p, and miR-378. Among them, miR-484 and miR-378 displayed the most significant inverse correlations with BMI (miR-484, r = −0.5484, P = 0.0056; miR-378, r = −0.5538, P = 0.0050) and visceral fat content (miR-484, r = −0.6141, P = 0.0014; miR-378, r = −0.6090, P = 0.0017). GO analysis indicated that genes targeted by miR-484 and miR-378 had major roles in carbohydrate and lipid metabolism. CONCLUSION Our result showed the differentially expressed circulating miRNAs in obese subjects compared to lean subjects. Although the mechanistic study to reveal the causal role of miRNAs remains, these miRNAs may be novel biomarkers for obesity.

show abstract

Gemcitabine impacts differentially on bladder and kidney cancer cells: distinct modulations in the expression patterns of apoptosis-related microRNAs and BCL2 family genes

Cited by 12 publications

References 50 publications

Immune-related long non-coding RNAs can serve as prognostic biomarkers for clear cell renal cell carcinoma

Immune-related long non-coding RNAs can serve as prognostic biomarkers for clear cell renal cell carcinoma

MicroRNA‑30a‑5p suppresses tumor cell proliferation of human renal cancer via the MTDH/PTEN/AKT pathway

Circulating microRNA expression profiling in young obese Korean women

Contact Info

Product

Resources

About