MicroRNA‑30a‑5p suppresses tumor cell proliferation of human renal cancer via the MTDH/PTEN/AKT pathway

Li, Jianmin; Li, Changying; Li, Hongjie; Zhang, Ting; Hao, Xiaodong; Chang, Jie; Xu, Yong

doi:10.3892/ijmm.2017.3269

Cited by 12 publications

(15 citation statements)

References 30 publications

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“…PTEN was the target of miR-28 and suppressed gastric cancer cell proliferation and invasion (41). Additionally, it suppressed renal cancer cell proliferation as a target of miR-30a (42). Zhao et al reported that PTEN regulated OS cell growth and apoptosis and acted as a target of miR-19 (43).…”

Section: Discussionmentioning

confidence: 99%

miR-29 promotes osteosarcoma cell proliferation and migration by targeting PTEN

et al. 2018

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Osteosarcoma (OS) is an aggressive malignant neoplasm that arises from primitively transformed cells of mesenchymal origin, and that exhibits osteoblastic differentiation and produces malignant osteoid. MicroRNAs (miRNAs) have been widely reported to have important regulatory roles in various human tumors, including OS. However, the potential mechanism of miR-29 in OS remains largely unknown. miR-29 was highly expressed in OS and overexpression of miR-29 promoted OS cell proliferation, as well as proliferating cell nuclear antigen (PCNA) expression and migration, whereas lower expression of miR-29 inhibited OS cell proliferation, PCNA expression and migration. In the present study, a dual-luciferase reporter system supporting phosphatase and tensin homolog (PTEN) was a target of miR-29 and its expression was inhibited by miR-29 mimic, but increased by miR-29 inhibitor. Overexpression of PTEN inhibited OS cell proliferation and migration and it could attenuate miR-29 promotion effect on OS progression. Overall, the results revealed that miR-29, as a tumor promoter, is involved in OS progression and metastasis by targeting PTEN, indicating that the miR-29/PTEN pathway is a potential therapeutic target for the treatment of OS.

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Section: Discussionmentioning

confidence: 99%

miR-29 promotes osteosarcoma cell proliferation and migration by targeting PTEN

et al. 2018

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“…Highly expressed MTDH was reported to serve important roles in the onset and the progression of OSCC by affecting tumor cell growth, colony formation, migration and invasion (26,28,29). MTDH has previously been reported to be involved in the regulation of the PTEN/AKT signaling pathway (23,24). It has been well established that activation of the PTEN/AKT pathway contributes to OSCC development (41,42).…”

Section: Discussionmentioning

confidence: 99%

“…Subsequent experiments were performed to determine whether MTDH was a direct target gene of miR-655 in OSCC cells. MTDH has previously been reported to contribute to the regulation of the phosphatase and tensin homolog (PTEN)/protein kinase (AKT) signaling pathway (23,24); therefore, the present study also investigated whether miR-655 participated in the regulation of the PTEN/AKT signaling pathway in OSCC cells.…”

Section: Microrna-655 Suppresses Cell Proliferation and Invasion In Omentioning

confidence: 99%

“…miR-655 overexpression inactivates the PTEN/AKT pathway in OSCC cells. MTDH has previously been reported to contribute to the regulation of the PTEN/AKT signaling pathway (23,24). To examine whether miR-655 was able to affect the PTEN/AKT pathway, the protein expression levels of PTEN, p-AKT and AKT were detected in Tca8113 and CAL-27 cells co-transfected with miR-655 mimics and either pCMV-MTDH or pCMV empty vector.…”

Section: Mir-655 Expression Is Inversely Correlated With Mtdh Mrna Exmentioning

confidence: 99%

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MicroRNA‑655 suppresses cell proliferation and invasion in oral squamous cell carcinoma by directly targeting metadherin and regulating the PTEN/AKT pathway

Wang

et al. 2018

Mol Med Report

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MicroRNAs (miRNAs) are important regulators of a variety of biological processes and their dysregulation is closely related to cancer formation and progression. Therefore, examination of aberrantly expressed miRNAs in oral squamous cell carcinoma (OSCC) may provide important clues for the diagnosis and treatment of patients with OSCC. The aim of the present study was to determine miRNA (miR)‑655‑3p expression in OSCC tissues and cell lines, and to investigate the biological roles and mechanisms of miR‑655‑3p associated with OSCC. Data from the present study indicated that miR‑655 expression was significantly downregulated in human OSCC tissues and cell lines. Overexpression of miR‑655 attenuated cell proliferation and invasion in OSCC in vitro. Metadherin (MTDH) mRNA was predicted as a potential target of miR‑655 by bioinformatics analysis, and this was confirmed by luciferase reporter assay, reverse transcription‑quantitative polymerase chain reaction and western blot analysis. In OSCC tissues, MTDH was highly expressed and inversely correlated with miR‑655 expression levels. MTDH overexpression reversed the inhibitory effects of miR‑655 mimics in OSCC cells. Notably, the upregulation of miR‑655 expression inhibited the activation of the phosphatase and tensin homolog (PTEN)/RAC‑α serine/threonine‑protein kinase (AKT) pathway in OSCC cells. Therefore, these results may provide the first evidence that miR‑655 targets MTDH to inhibit proliferation and invasion of OSCC by inhibiting PTEN/AKT signaling. Thus, the restoration of miR‑655 expression may be a novel therapeutic strategy for patients with OSCC.

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“…It was reported that MTDH is expressed in almost all malignant tumor cells. MTDH overexpression can enhance the proliferation, differentiation, metastasis and invasion capacity of endometrial cancer, prostate cancer, breast cancer and human glioma cells (18). In addition, MTDH plays a key role in upregulating tumor genesis mediated by the Ha-as oncogene (19).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‑98/PTEN/AKT pathway inhibits cell proliferation and malignant progression of hypopharyngeal carcinoma by MTDH

et al. 2018

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Laryngeal carcinoma is one of the most common tumors concerning otorhinolaryngology head and neck surgery, however, the pathogenesis of laryngeal carcinoma remains unclear. MicroRNAs (miRNAs) have been reported to play vital roles in the pathogenesis of laryngeal carcinoma Herein, the present study was designed to explore the function and mechanism of miRNA-98 in hypopharyngeal carcinoma. In brief, qRT-PCR, MTT assay, western blot analysis, Transwell assay and luciferase reporter assay were performed. Based on the results, miRNA-98 expression was downregulated in patients with hypopharyngeal carcinoma. Downregulation of miRNA-98 promoted cell growth and migration, and decreased the apoptotic rate of hypopharyngeal carcinoma cells. Overexpression of miRNA-98 increased the apoptotic rate, and inhibited cell growth and migration of hypopharyngeal carcinoma cells. Moreover, luciferase reporter assays revealed that MTDH is a direct target of miRNA-98 and overexpression of miRNA-98 induced the protein expression of PTEN and suppressed that of PI3K and p-Akt. si-MTDH attenuated the anticancer effects of miRNA-98 on hypopharyngeal carcinoma via the PTEN/AKT pathway. To the best of our knowledge, the present study confirmed for the first time that miRNA-98 inhibits hypopharyngeal carcinoma cell proliferation and induces apoptosis via the PTEN/AKT pathway by MTDH.

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MicroRNA‑30a‑5p suppresses tumor cell proliferation of human renal cancer via the MTDH/PTEN/AKT pathway

Cited by 12 publications

References 30 publications

miR-29 promotes osteosarcoma cell proliferation and migration by targeting PTEN

miR-29 promotes osteosarcoma cell proliferation and migration by targeting PTEN

MicroRNA‑655 suppresses cell proliferation and invasion in oral squamous cell carcinoma by directly targeting metadherin and regulating the PTEN/AKT pathway

MicroRNA‑98/PTEN/AKT pathway inhibits cell proliferation and malignant progression of hypopharyngeal carcinoma by MTDH

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