Gelation of hyaluronic acid through annealing

Fujiwara, Junji; Takahashi, Masato; Hatakeyama, Tatsuko; Hatakeyama, Hyōe

doi:10.1002/1097-0126(200012)49:12<1604::aid-pi558>3.0.co;2-4

Cited by 24 publications

(12 citation statements)

References 10 publications

(13 reference statements)

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“…which is observed until conformational equilibration is achieved 42) . The hyaluronan sol (3%) is annealed at 333K for various time intervals from 0 to 50 hours, and W nf was calculated from the enthalpy of melting.…”

Section: Structural Change Of Hydrogelsmentioning

confidence: 99%

“…After annealing, gelation occurs by cooling. Hyaluronan 42,83,84) and xanthan gum [85][86][87][88][89] form weak gels by this method.…”

Section: Analytical Sciencesmentioning

confidence: 99%

“…the conformational change via hydrogelation of polysaccharides. 19,22,[35][36][37][38][39][40][41] Classical experimental methods, such as dynamic water swelling measurement, 16) tea-bag measurement 16) and falling ball method 42) are still useful tools in order to understand whole nature of polysaccharide hydrogels.…”

mentioning

confidence: 99%

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DSC and TMA Studies of Polysaccharide Physical Hydrogels

Iijima

Hatakeyama²,

Hatakeyama³

2020

ANAL. SCI.

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Various kinds of polysaccharides found in a wide variety of plants, bacteria, crustaceans and insects form hydrogels via physical aggregation in aqueous media. The major mass of hydrogels is water filled, ca. 95~99.5 %, in a network structure, although the solid shape of the gel is maintained. In this paper, firstly the wide range of gelation mechanism is briefly described, and then thermal analysis of representative gel-forming polysaccharides, such as carrageenan, alginate, galactomannan, and pectin, is introduced. By differential scanning calorimetry (DSC), gel-sol and sol-gel transition temperature of thermoreversible hydrogels are measured and phase diagram is established. It is suggested that binary systems showing sinusoidal gel-sol-gel transition are capable of being assembled. By thermomechanical analysis (TMA), dynamic modulus (E') at around 1 x10 4 Pa of thermo-irreversible hydrogels was obtained using a sample holder designed to measure the viscoelastic properties in water. Reliable coordination is shown between the results obtained by DSC and TMA. In this review, current research and several topics on thermal properties of polysaccharide physical hydrogels are introduced.

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Section: Structural Change Of Hydrogelsmentioning

confidence: 99%

“…After annealing, gelation occurs by cooling. Hyaluronan 42,83,84) and xanthan gum [85][86][87][88][89] form weak gels by this method.…”

Section: Analytical Sciencesmentioning

confidence: 99%

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DSC and TMA Studies of Polysaccharide Physical Hydrogels

Iijima

Hatakeyama²,

Hatakeyama³

2020

ANAL. SCI.

Self Cite

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“…In case of supramolecular gels, thermal annealing at a temperature above the melting temperature of the gel results in sol formation and re-gelling on cooling. [53][54][55][56] Thermal annealing results in more homogeneous network formation 56 by eliminating the errors in the initial assembly states and often causes substantial increase in robustness of the gels. [57][58][59] Recently, thermal annealing has been shown to be also applicable to tune the material properties for multicomponent gel systems ( Fig.…”

Section: (I) Thermal Annealing Of Supramolecular Gelsmentioning

confidence: 99%

Stimuli responsive dynamic transformations in supramolecular gels

2021

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“…In one case (LS1), the viscosity after freezing increases in relation to a reinforcement of the network junctions, as observed previously in some polysaccharide physical gels. Self association of HA was mentioned in NaCl aqueous solution [33] but also at low temperatures [34,35]. For the SF named LS2, there is no modification whatsoever.…”

Section: Stability Of Linear Ha and Sf At 4 And −21 °Cmentioning

confidence: 99%

Role of Different Pre-Treatments on Composition and Rheology of Synovial Fluids

Rinaudo

Rozand²,

Mathieu

et al. 2009

Polymers

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Different pre-treatments applied to synovial fluids (SF) before their analyses are tested to characterize SF after storage under different conditions and to investigate their evolution along a viscosupplementation treatment. The main techniques proposed involve steric exclusion chromatography with triple detection (SEC) and viscometry; it is the first time that such a study is developed. SEC gives the molecular weight distribution and concentration of hyaluronan (HA) and proteins separately; the steady state viscosity is always non-Newtonian and not directly related to SF composition. Pre-treatment of SF (storage in cold, filtration, centrifugation) allows us to conclude that, in order to store SF, it is best to freeze it, even if in some cases, viscosity is modified but not the composition. All the data obtained (including protease pre-treatment) allow us to conclude that a small fraction of HA-protein complex forms a loose 3D-network and controls the rheology.

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Gelation of hyaluronic acid through annealing

Cited by 24 publications

References 10 publications

DSC and TMA Studies of Polysaccharide Physical Hydrogels

DSC and TMA Studies of Polysaccharide Physical Hydrogels

Stimuli responsive dynamic transformations in supramolecular gels

Role of Different Pre-Treatments on Composition and Rheology of Synovial Fluids

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