Gelatin stabilization of quantum dots for improved stability and biocompatibility

Parani, Sundararajan; Pandian, K.; Oluwafemi, Oluwatobi S.

doi:10.1016/j.ijbiomac.2017.09.039

Cited by 29 publications

(13 citation statements)

References 37 publications

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“…compared to organic fluorophores and have led to their use in a variety of bio-applications such as bio-imaging, diagnostics, drug delivery, in-vitro single molecule sensing and in-vivo cell tracking [3][4][5] . Most of the reported studies on QDs have focused on binary semiconductor nanocrystals with many advances being reported with regards to their synthesis and application 6,7 . Despite the vast amount of work reported on the application of binary QDs by various groups 8,9 , the clinical use of the binary QDs has been limited by the presence of toxic heavy metals (Cd, Pb, Hg) in their composition.…”

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confidence: 99%

Cytotoxicity, fluorescence tagging and gene-expression study of CuInS/ZnS QDS - meso (hydroxyphenyl) porphyrin conjugate against human monocytic leukemia cells

Tsolekile

Nahle

Zikalala

et al. 2020

Sci Rep

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The toxicity of heavy metals present in binary semiconductor nanoparticles also known as quantum dots (QDs) has hindered their wide applications hence the advent of non-toxic ternary quantum dots. These new group of quantum dots have been shown to possess some therapeutic action against cancer cell lines but not significant enough to be referred to as an ideal therapeutic agent. In this report, we address this problem by conjugating red emitting CuInS/ZnS QDs to a 5,10,15,20-tetrakis(3hydroxyphenyl)porphyrin-photosensitizer for improved bioactivities. The glutathione capped CuInS/ ZnS QDs were synthesized in an aqueous medium using a kitchen pressure cooker at different Cu: In ratios (1:4 and 1:8) and at varied temperatures (95 °C, 190 °C and 235 °C). Optical properties show that the as-synthesized CuInS/ZnS QDs become red-shifted compared to the core (CuInS) after passivation with emission in the red region while the cytotoxicity study revealed excellent cell viability against normal kidney fibroblasts (BHK21). The highly fluorescent, water-soluble QDs were conjugated to 5,10,15,20-tetrakis(3-hydroxyphenyl)porphyrin (mTHPP) via esterification reactions at room temperature. The resultant water-soluble conjugate was then used for the cytotoxicity, fluorescent imaging and gene expression study against human monocytic leukemia cells (THP-1). Our result showed that the conjugate possessed high cytotoxicity against THP-1 cells with enhanced localized cell uptake compared to the bare QDs. In addition, the gene expression study revealed that the conjugate induced inflammation compared to the QDs as NFKB gene was over-expressed upon cell inflammation while the singlet oxygen (1 o 2) study showed the conjugate possessed large amount of 1 o 2 , three times than the bare porphyrin. Thus, the as-synthesized conjugate looks promising as a therapeutic agent for cancer therapy. The use of nanomaterials for biological application has in the past decade transpired as a promising avenue to explore for biological application due to their size, shape, specific surface area, aspect ratio and surface chemistry. Of the reported nanomaterial, semiconductor nanomaterials also known as quantum dots (QDs) still remain the most promising due to their excellent optoelectronic properties and spectral tunability which allows for their use in the ultraviolet and near infra-red region 1,2. These inherent properties have made QDs more appealing

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confidence: 99%

Cytotoxicity, fluorescence tagging and gene-expression study of CuInS/ZnS QDS - meso (hydroxyphenyl) porphyrin conjugate against human monocytic leukemia cells

Tsolekile

Nahle

Zikalala

et al. 2020

Sci Rep

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“…Although QDs are known for their toxicity, this still does not frustrate the enthusiasm of scientists. Researchers have been working to reduce their toxicity in a variety of ways [64,65]. In our study, a novel drug-targeted delivery system was used to trace glioma by combining as many QDs as possible with each glioma cell to achieve accurate positioning of glioma boundaries.…”

Section: Discussionmentioning

confidence: 99%

c(RGDyk)-modified nanoparticles encapsulating quantum dots as a stable fluorescence probe for imaging-guided surgical resection of glioma under the auxiliary UTMD

Xiong

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Surgical resection remains the preferred approach for some patients with glioblastoma (GBM), and eradication of the residual tumour niche after surgical resection is very helpful for prolonging patient survival. However, complete surgical resection of invasive GBM is difficult because of its ambiguous boundary. Herein, a novel targeting material, c(RGDyk)-poloxamer-188, was synthesized by modifying carboxyl-terminated poloxamer-188 with a glioma-targeting cyclopeptide, c(RGDyk). Quantum dots (QDs) as fluorescent probe were encapsulated into the self-assembled c(RGDyk)-poloxamer-188 polymer nanoparticles (NPs) to construct glioma-targeted QDs-c(RGDyk)NP for imaging-guided surgical resection of GBM. QDs-c(RGDyk)NP exhibited a moderate hydrodynamic diameter of 212.4 nm, a negative zeta potential of-10.1 mV and good stability. QDs-c(RGDyk)NP exhibited significantly lower toxicity against PC12 and C6 cells and HUVECs than free QDs. Moreover, in vitro cellular uptake experiments demonstrated that QDs-c(RGDyk)NP specifically targeted C6 cells, making them display strong fluorescence. Combined with ultrasound-targeted microbubble destruction (UTMD), QDs-c(RGDyk)NP specifically accumulated in glioma tissue in orthotropic tumour rats after intravenous administration, evidenced by ex vivo NIR fluorescence imaging of bulk brain and glioma tissue sections. Furthermore, fluorescence imaging with QDs-c(RGDyk)NP guided accurate surgical resection of glioma. Finally, the safety of QDs-c(RGDyk)NP was verified using pathological HE staining. In conclusion, QDs-c(RGDyk)NP may be a potential imaging probe for imaging-guided surgery.

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“…The micro-strain is estimated via the expression [31] and the stress σ(GPa) in the crystallite's plane [32] are computed (5) (6) where c and cbulk are the measured lattice parameter and the strain-free lattice parameter of ZnO (5.2061 Å) respectively. The Zn -O bond length (L) was calculated using the equation: (7) where…”

Section: Xrd Analysismentioning

confidence: 99%

“…For instance, ZnO nano-plates were developed from ZnO nanoparticles using gelatin [6], while Bauermann et al fabricated hexagonal ZnO plates in a gelatin matrix [5]. Gelatin was employed to stabilize CdTe/CdS/ZnS (CSSG) core/double shell quantum dots (QDs) to advance their biocompatibility [7], copper NPs [8] and ZnO NPs [9].…”

Section: Introductionmentioning

confidence: 99%

TiO2–ZnO composites fabricated via sonication assisted with gelatin for potential use in Rhodamine B degradation

Toghan

Taha

Modwi

2021

J Mater Sci: Mater Electron

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Herewith we report a facile synthesis of zinc oxide doped with (5, 10, 15, and 20 wt%) titanium oxide nanocomposites in gelatin under ultra-sonication. The X-ray diffraction (XRD) data revealed ZnO the formation in addition to a rutile phase TiO2. The ZnO phase size decreased, and the rutile TiO2 phase increased with a TiO2 loading increment. The scanning electron microscopy (SEM) displayed a combination of spherical and hexagonal particles with a 60 -80 nm size distribution. The prepared nanostructures photocatalytic activity was assisted using Rhodamine B dye, where they showed enhanced photodegradation competence under visible light irradiation. The kinetics of photodegradation followed the first-order kinetics with the 20 % wt sample having the maximum activity. The mechanistic investigation revealed the dominance of h + and •O2species during the dye photodegradation. The results indicate the potential application of such gelatin stabilized nanostructures for dye illumination from aqueous solutions under sunlight.

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Gelatin stabilization of quantum dots for improved stability and biocompatibility

Cited by 29 publications

References 37 publications

Cytotoxicity, fluorescence tagging and gene-expression study of CuInS/ZnS QDS - meso (hydroxyphenyl) porphyrin conjugate against human monocytic leukemia cells

Cytotoxicity, fluorescence tagging and gene-expression study of CuInS/ZnS QDS - meso (hydroxyphenyl) porphyrin conjugate against human monocytic leukemia cells

c(RGDyk)-modified nanoparticles encapsulating quantum dots as a stable fluorescence probe for imaging-guided surgical resection of glioma under the auxiliary UTMD

TiO2–ZnO composites fabricated via sonication assisted with gelatin for potential use in Rhodamine B degradation

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