Gelatin-Methacryloyl Hydrogels: Towards Biofabrication-Based Tissue Repair

Klotz, Barbara; Gawlitta, Debby; Rosenberg, Antoine J.W.P.; Malda, Jos; Melchels, Ferry P.W.

doi:10.1016/j.tibtech.2016.01.002

Cited by 632 publications

(550 citation statements)

References 93 publications

(130 reference statements)

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“…The bands at 1451 and 1381 cm −1 corresponded to the C-H scissor bending vibrations [32,33]. Compared with the spectrum of PDLLA/nHA composite fibrous membranes, seen from Figure 5b,c, the specific adsorption peaks of PO 4 3− at 1033, 603 and 564 cm −1 were found [32]. And the typical adsorption peaks of PDLLA were not changed, demonstrating that the adsorption of the nHA particles and the shrinking of the mats occurred via all physical processes.…”

Section: Ftir Of Pdlla/nha Composite Fibrous Membranementioning

confidence: 93%

Electrospun Porous PDLLA Fiber Membrane Coated with nHA

Qiang

Zhang²,

Feng³

et al. 2018

Applied Sciences

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Featured Application: The PDLLA/nHA composite fibrous membrane has potential application value as scaffold material in tissue engineering.Abstract: Porous poly-D, L-lactic acid (PDLLA) electrospinning fiber membrane was prepared, and nano-hydroxyapatite (nHA) was adsorbed and wrapped into it during the unique shrinking process of the PDLLA fiber membrane to fabricate the PDLLA/nHA composite membrane scaffold for tissue engineering. Compare with the composite fibers prepared by blend electrospinning, most of nHA particles are observed to distribute on the surface of new type composite fibers, which could significantly improve the water wettability and induce the cellular adherence. FTIR analysis indicated that the PDLLA/nHA composite fibrous membrane was formed by physical adsorption. The combination was probed by scanning electron microscope, thermo-gravimetric, water contact angle and mechanical property analysis. It was proved that the nHA particles' content and distribution, surface wettability, modulus and tensile strength of PDLLA/nHA composite fibrous membrane were influenced by the concentration of nHA dispersion and pores on the PDLLA fiber surface. The 10.6 wt % PDLLA/nHA composite fibrous membrane exhibits a more balanced tensile strength (3.28 MPa) and surface wettability (with a water contact angle of 0 • ) of the composite mats. Scanning electron microscope and confocal laser scanning microscopy images of chondrocyte proliferation further showed that the composite scaffold is non-toxic. The adherence and proliferation of chondrocytes on the 10.6 wt % PDLLA/nHA fibrous membrane was significantly improved, compared with PDLLA mat. The 10.6 wt % PDLLA/nHA composite fibrous membrane has potential application value as scaffold material in tissue engineering.

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Section: Ftir Of Pdlla/nha Composite Fibrous Membranementioning

confidence: 93%

Electrospun Porous PDLLA Fiber Membrane Coated with nHA

Qiang

Zhang²,

Feng³

et al. 2018

Applied Sciences

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“…In addition, hydrogels can be relatively easily modified to achieve desired topographies, stiffness and biofunctional characteristics (e.g. arginine-glycine-aspartic acid (RGD) sites for cell attachment) 19 .…”

Section: Introductionmentioning

confidence: 99%

“…Only a few studies have looked specifically at the response of macrophages to hydrogels 23 . Gelatin methacryloyl (GelMA) hydrogels are becoming increasingly popular as they combine the bioactivity of gelatin with the tunability of a photo-crosslinkable hydrogel 19,24 . Since…”

Section: Introductionmentioning

confidence: 99%

Unbiased Analysis of the Impact of Micropatterned Biomaterials on Macrophage Behavior Provides Insights beyond Predefined Polarization States

Singh

Awuah

Rostam

et al. 2017

ACS Biomater. Sci. Eng.

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ABSTRACT:Macrophages are master regulators of immune responses towards implanted biomaterials. The activation state adopted by macrophages in response to biomaterials determines their own phenotype and function as well as those of other resident and infiltrating immune and non-immune cells in the area. A wide spectrum of macrophage activation states exists, with M1 (pro-inflammatory) and M2 (anti-inflammatory) representing either ends of the spectrum. In biomaterials research, cellinstructive surfaces that favour or induce M2 macrophages have been considered as beneficial due to the anti-inflammatory and pro-regenerative properties of these cells. In this study, we used a gelatin methacryloyl (GelMA) hydrogel platform to determine whether micropatterned surfaces can modulate the phenotype and function of human macrophages. The effect of microgrooves/ridges and micropillars on macrophage phenotype, function, and gene expression profile were assessed using conventional methods (morphology, cytokine profile, surface marker expression, phagocytosis) and gene microarrays.Our results demonstrated that micropatterns did induce distinct gene expression profiles in human macrophages cultured on microgrooves/ridges and micropillars. Significant changes were observed in genes related to primary metabolic processes such as transcription, translation, protein trafficking, DNA repair and cell survival. However, interestingly conventional phenotyping methods, relying on surface marker expression and cytokine profile, were not able to distinguish between the different conditions, and indicated no clear shift in cell activation towards an M1 or M2 phenotypes. This highlights the limitations of studying the effect of different physicochemical conditions on macrophages by solely relying on conventional markers that are primarily developed to differentiate between cytokine polarised M1 and M2 macrophages. We therefore, propose the adoption of unbiased screening methods in determining macrophage responses to biomaterials. Our data clearly shows that the exclusive use of conventional markers and methods for determining macrophage activation status could lead to missed opportunities for understanding and exploiting macrophage responses to biomaterials.

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“…To maximally simulate a GBM resection, as well as validate the cytotoxicity of the implant to human U87 cells in vitro, we engineered a 3D GelMA scaffold in the shape of a resection tumor cavity 11. Then, we cultured human U87 cells on the scaffold to replicate the glioma residual cavity ex vivo, and the shape‐matched implant was then inserted into the glioma residual cavity.…”

Section: Resultsmentioning

confidence: 99%

A 3D‐Engineered Conformal Implant Releases DNA Nanocomplexs for Eradicating the Postsurgery Residual Glioblastoma

Yang

Kang

et al. 2017

Advanced Science

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Gene therapy has great promise for glioblastoma treatment; however, it remains a great challenge to efficiently deliver genes to the brain. The incomplete resection of glioblastoma always leads to poor prognosis. Here, a 3D‐engineered conformal implant for eradicating the postsurgery residual glioblastoma is designed. This implant is constructed by 3D‐printing technology to match the tumor cavity and release an oncolytic virus‐inspired DNA nanocomplex to kill glioblastoma cells through apoptosis induction. Meanwhile, a 3D‐engineered subcutaneous glioblastoma xenograft is built to mimic the resection tumor cavity in mice. Insertion of the implant into the glioblastoma resection cavity efficiently delays tumor recurrence and significantly prolongs overall survival. This study provides a proof‐of‐concept of glioblastoma therapy using a conformal implant that releases oncolytic DNA nanocomplexs. This strategy can lead to the development of future precision therapy for eradicating postsurgery residual tumors.

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Gelatin-Methacryloyl Hydrogels: Towards Biofabrication-Based Tissue Repair

Cited by 632 publications

References 93 publications

Electrospun Porous PDLLA Fiber Membrane Coated with nHA

Electrospun Porous PDLLA Fiber Membrane Coated with nHA

Unbiased Analysis of the Impact of Micropatterned Biomaterials on Macrophage Behavior Provides Insights beyond Predefined Polarization States

A 3D‐Engineered Conformal Implant Releases DNA Nanocomplexs for Eradicating the Postsurgery Residual Glioblastoma

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