Gelatin-CaO2/SAP/PLGA composite scaffold enhances the reparation of critical-sized cranial defects by promoting seed cell survival

Zhang, Zhiming; Rong, Zijie; Wu, Guofeng; Wang, Yihan; Tan, Zhiwen; Zheng, Juan; Jin, Yuqi; Liang, Zhonglin; Li, Chun; Guo, Jiasong; Zhu, Lixin

doi:10.1016/j.apmt.2021.100960

Cited by 11 publications

(14 citation statements)

References 50 publications

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“…Preparation of the gelatin/CaO 2 microspheres was performed by an emulsion cross-linking method according to a previous report . Gelatin (Sigma-Aldrich, USA) was dissolved in deionized water under hydrothermal conditions to obtain 80 wt % gelatin solution.…”

Section: Methodsmentioning

confidence: 99%

“…The performance of solid inorganic peroxides such as sodium percarbonate is limited by the rapid degradation rate, local cellular oxygen toxicity caused by rapid and massive oxygen release, poor mechanical strength, difficulty in molding, and the need for secondary processing . As previously shown, a slow, sustained release of oxygen-releasing gelatin-CaO 2 microspheres was prepared and demonstrated to promote osteogenesis in vivo . However, manufacturing porous or shape-matching scaffolds from pure solid inorganic perovskites is challenging and frequently requires secondary processes, such as chemical or physical methods of coating .…”

Section: Introductionmentioning

confidence: 99%

“…12 As previously shown, a slow, sustained release of oxygen-releasing gelatin-CaO 2 microspheres was prepared and demonstrated to promote osteogenesis in vivo. 13 However, manufacturing porous or shape-matching scaffolds from pure solid inorganic perovskites is challenging and frequently requires secondary processes, such as chemical or physical methods of coating. 14 Three-dimensional (3D) printing approaches consisting of fused deposition modeling, selective laser melting, selective laser sintering, and laser 3D printing are frequently employed for the creation of tissue engineering scaffolds, 15 for excellent integration of different components to produce integrated functional scaffolds and the ability to fabricate scaffolds to match the specific anatomical geometry of the patient with a bone defect.…”

Section: Introductionmentioning

confidence: 99%

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3D Printed Integrated Bionic Oxygenated Scaffold for Bone Regeneration

Wang

Xie

Zhang

et al. 2022

ACS Appl. Mater. Interfaces

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The repair of large bone defects remains a challenging problem in bone tissue engineering. Ischemia and hypoxia in the bone defect area make it difficult for seed cells to survive and differentiate, which fail to perform effective tissue regeneration. Current oxygen-producing materials frequently encounter problems such as a rapid degradation rate, insufficient mechanical properties, difficult molding, and cumbersome fabrication. Here, a novel three-dimensional (3D) printed integrated bionic oxygenated scaffold was fabricated with gelatin-CaO2 microspheres, polycaprolactone (PCL), and nanohydroxyapatite (nHA) using low-temperature molding 3D printing technology. The scaffold had outstanding mechanical properties with bionic hierarchical porous structures. In vitro reports showed that the scaffold exhibited excellent cytocompatibility and could release O2 sustainably for more than 2 weeks, which significantly enhanced the survival, growth, and osteogenic differentiation of bone marrow mesenchymal stem cells under hypoxia. In vivo experiments revealed that the scaffold facilitated efficient bone repair after it was transplanted into a rabbit calvarial defect model. This result may be due to the scaffolds reducing hypoxia-inducible factor-1α accumulation, improving the expression of osteogenic regulatory transcription factors, and accelerating osteogenesis. In summary, the integrated bionic PCL/nHA/CaO2 scaffold had excellent capabilities in sustainable O2 release and bone regeneration, which provided a promising clinical strategy for bone defect repair.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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3D Printed Integrated Bionic Oxygenated Scaffold for Bone Regeneration

Wang

Xie

Zhang

et al. 2022

ACS Appl. Mater. Interfaces

Self Cite

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“…On the other hand, gelatin is known for its ability to absorb water. This characteristic is highly valued in tissue regeneration, since porosity ensures a diffusion of nutrients as well as oxygen for proper cell growth [36]. However, porous structures do not always meet all the requirements to provide the exchange of products for cell survival, because either the size or the diameter of the pores is not sufficient, or they are not regular enough, and some gelatin-based cell delivery systems have demonstrated a poor cell survival rate [37].…”

Section: Gelatin As a Systemmentioning

confidence: 99%

“…In that case, temperature cooling helps gelatin to form porous structures and thus better mimic tissue and promote nerve regeneration [12]. Furthermore, this technique has become widespread, as it has also proven to be advantageous for cell protection as well as for the release of biologically active agents [17,36,54,55]. The freeze-drying technique enabled gelatin to form a porous hydrogel, which helped to attract cells responsible for osteogenesis [18,30].…”

Section: Freeze-drying Techniquementioning

confidence: 99%

Progress in Gelatin as Biomaterial for Tissue Engineering

et al. 2022

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Tissue engineering has become a medical alternative in this society with an ever-increasing lifespan. Advances in the areas of technology and biomaterials have facilitated the use of engineered constructs for medical issues. This review discusses on-going concerns and the latest developments in a widely employed biomaterial in the field of tissue engineering: gelatin. Emerging techniques including 3D bioprinting and gelatin functionalization have demonstrated better mimicking of native tissue by reinforcing gelatin-based systems, among others. This breakthrough facilitates, on the one hand, the manufacturing process when it comes to practicality and cost-effectiveness, which plays a key role in the transition towards clinical application. On the other hand, it can be concluded that gelatin could be considered as one of the promising biomaterials in future trends, in which the focus might be on the detection and diagnosis of diseases rather than treatment.

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Chemically Programmed Hydrogels for Spatiotemporal Modulation of the Cardiac Pathological Microenvironment

Yu,

Qiu,

Yao

et al. 2024

Advanced Materials

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After myocardial infarction (MI), sustained ischemic events induce pathological microenvironments characterized by ischemia‐hypoxia, oxidative stress, inflammatory responses, matrix remodeling, and fibrous scarring. Conventional clinical therapies lack spatially targeted and temporally responsive modulation of the infarct microenvironment, leading to limited myocardial repair. Engineered hydrogels have a chemically programmed toolbox for minimally invasive localization of the pathological microenvironment and personalized responsive modulation over different pathological periods. Chemically programmed strategies for crosslinking interactions, interfacial binding, and topological microstructures in hydrogels enable minimally invasive implantation and in situ integration tailored to the myocardium. This enhances substance exchange and signal interactions within the infarcted microenvironment. Programmed responsive polymer networks, intelligent micro/nanoplatforms, and biological therapeutic cues contribute to the formation of microenvironment‐modulated hydrogels with precise targeting, spatiotemporal control, and on‐demand feedback. Therefore, this review summarizes the features of the MI microenvironment and chemically programmed schemes for hydrogels to conform, integrate, and modulate the cardiac pathological microenvironment. Chemically programmed strategies for oxygen‐generating, antioxidant, anti‐inflammatory, provascular, and electrointegrated hydrogels to stimulate iterative and translational cardiac tissue engineering are discussed.This article is protected by copyright. All rights reserved

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Gelatin-CaO2/SAP/PLGA composite scaffold enhances the reparation of critical-sized cranial defects by promoting seed cell survival

Cited by 11 publications

References 50 publications

3D Printed Integrated Bionic Oxygenated Scaffold for Bone Regeneration

3D Printed Integrated Bionic Oxygenated Scaffold for Bone Regeneration

Progress in Gelatin as Biomaterial for Tissue Engineering

Chemically Programmed Hydrogels for Spatiotemporal Modulation of the Cardiac Pathological Microenvironment

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