Gelam Honey Inhibits the Production of Proinflammatory, Mediators NO,PGE2, TNF-α, and IL-6 in Carrageenan-Induced Acute Paw Edema in Rats

Abstract

Natural honey is well known for its therapeutic value and has been used in traditional medicine of different cultures throughout the world. The aim of this study was to investigate the anti-inflammatory effect of Malaysian Gelam honey in inflammation-induced rats. Paw edema was induced by a subplantar injection of 1% carrageenan into the rat right hind paw. Rats were treated with the nonsteroidal anti-inflammatory drug (NSAID) Indomethacin (10 mg/kg, p.o.) or Gelam honey at different doses (1 or 2 g/kg, p.o.). The increase in footpad thickness was considered to be edema, which was measured using a dial caliper. Plasma and paw tissue were collected to analyze the production of inflammatory mediators, such as NO, PGE2, TNF-α, and IL-6, as well as iNOS and COX-2. The results showed that Gelam honey could reduce edema in a dose-dependent fashion in inflamed rat paws, decrease the production of NO, PGE2, TNF-α, and IL-6 in plasma, and suppress the expression of iNOS, COX-2, TNF-α, and IL-6 in paw tissue. Oral pretreatment of Gelam honey at 2 g/kg of body weight at two time points (1 and 7 days) showed a significantly decreased production of proinflammatory cytokines, which was similar to the effect of the anti-inflammatory drug Indomethacin (NSAID), both in plasma and tissue. Thus, our results suggest that Gelam honey has anti-inflammatory effects by reducing the rat paw edema size and inhibiting the production of proinflammatory mediators. Gelam honey is potentially useful for treating inflammatory conditions.

Gelam Honey and Ginger Potentiate the Anti Cancer Effect of 5-FU against HCT 116 Colorectal Cancer Cells

“…Studies have indicated the range of health benefits of honey, which includes anti-inflammatory, antioxidant and wound healing activities (Kassim et al, 2010;Mohamed et al, 2010;Al-Waili et al, 2011;Hussein et al, 2012). Honey was also known to exert anti-cancer effects in several types of cancer cells including colon cancer Fukuda et al, 2011).…”

Effect of Topical Honey on Mandibular Bone Defect Healing in Rats

“…A study showed that honey flavonoid extract significantly suppressed the secretion of proinflammatory cytokines including tumor necrosis factoralpha (TNF-α) and interleukin (IL)-1 beta. 20 In an in vivo study on rats, Hussein et al 21 showed that honey could decrease edema and also decrease the levels of inflammatory mediators including prostaglandin E2, TNF-α, nitric oxide, and IL-6 in the plasma. Its anti-inflammatory effect was similar to that of indomethacin.…”

“…17 The antibacterial and anti-inflammatory effects of honey have been reported in several studies. [18][19][20][21][22][23] In addition, honey contains vitamin C concentration three times more than plasma, 24 which could accelerate tissue regeneration. 25 A recent clinical trial showed the effectiveness of honey as a dressing for dry socket.…”

Anti-inflammatory activity and modulate oxidative stress of Bucida buceras in lipopolysaccharide-stimulated RAW 264.7 macrophages and Carrageenan-induced acute paw edema in rats

“…Paw edema induced by subcutaneous injection of λ-carrageenan into the right hind paw of rats, as was described previously by Hussein et al (2012). The anti-inflammatory effect of aqueous B. buceras extract was evaluated by decrease in percentage of inhibition of the paw thickness.…”

“…In this study would indicate that the inhibition of the paw edema could be due to its antioxidative and oxygen free radical scavenger properties. Edema is one of the fundamental actions of acute inflammation and is an essential parameter to be considered when evaluating compounds with potential anti-inflammatory activity (Hussein et al, 2012).…”