Gelam Honey Attenuates the Oxidative Stress‐Induced Inflammatory Pathways in Pancreatic Hamster Cells

Abstract

Purpose. Type 2 diabetes consists of progressive hyperglycemia and insulin resistance, which could result from glucose toxicity, inflammatory cytokines, and oxidative stress. In the present study we investigated the effect of Gelam honey and quercetin on the oxidative stress-induced inflammatory pathways and the proinflammatory cytokines. Methods. HIT-T15 cells were cultured and preincubated with the extract of Gelam honey (20, 40, 60, and 80 μg/mL), as well as quercetin (20, 40, 60, and 80 μM), prior to stimulation by 20 and 50 mM glucose. Results. HIT-T15 cells cultured under hyperglycemic condition showed a significant increase in the inflammatory pathways by phosphorylating JNK, IKK-β, and IRS-1 at Ser307 (p < 0.05). There was a significant decrease in the phosphorylation of Akt at Ser473 (p < 0.05). Pretreatment with Gelam honey and quercetin reduced the expression of phosphorylated JNK, IKK-β, and IRS-1, thereby significantly reducing the expression of proinflammatory cytokines like TNF-α, IL-6, and IL-1β (p < 0.05). At the same time there was a significant increase in the phosphorylated Akt showing the protective effects against inflammation and insulin resistance (p < 0.05). In conclusion, our data suggest the potential use of the extract from Gelam honey and quercetin in modulating the inflammation induced insulin signaling pathways.

CFTR ameliorates high glucose-induced oxidative stress and inflammation by mediating the NF-κB and MAPK signaling pathways in endothelial cells

“…Furthermore, to delineate the mechanism behind high glucose-induced inflammation, given the reported involvement of oxidative stress in regulating inflammation (7,8), the role of ROS was examined. The present study showed that high glucose decreased CFTR expression, which exhibited a reciprocal pattern of concentration-dependent ROS production.…”

“…ROS measurement. ROS level in the HUVECs was measured using 2' ,7'-dichlorofluorescin diacetate (H 2 DCF-DA; Invitrogen; Thermo Fisher Scientific, Inc.), as previously described (7). Briefly, HUVECs (2x10 4 cells/well) were seeded in 96-well clear-bottom black plates and then incubated with different concentrations (10, 15, 20 and 25 mM) of glucose for 24 h at 37˚C.…”

“…In addition, high glucose-induced oxidative stress, primarily associated with the production of reactive oxygen species (ROS), also serves a pivotal role in endothelial dysfunction during the development of diabetes (6). ROS function as signaling molecules to activate a variety of stress pathways and inflammatory pathways, which in turn further enhance inflammatory responses (7,8). Furthermore, inhibition of ROS with antioxidant agents can attenuate inflammation and rescue endothelial cells (9).…”

Physicochemical and Medicinal Properties of Tualang, Gelam and Kelulut Honeys: A Comprehensive Review

“…Gelam honey had been shown to attenuate the oxidative stress-induced inflammatory pathways when pretreatment with Gelam honey or quercetin reduced the expression of phosphorylated jun-N-terminal kinase, IkappaB kinase and IRS-1, thereby significantly reducing the expression of proinflammatory cytokines, such as tumour necrosis factor alpha (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β). A significant increase in phosphorylated Akt was also found, indicating the protective effects against inflammation and insulin resistance [ 46 ]. Batumalaie et al (2014) reported that pretreatment of HIT-T15 cells with Gelam honey before culturing in hyperglycemic media yielded a significant decrease in the production of reactive oxygen species (ROS), glucose-induced lipid peroxidation and a significant increase in insulin content and the viability of cells cultured under hyperglycemic condition [ 47 ].…”

Therapeutic Properties of Stingless Bee Honey in Comparison with European Bee Honey

“…EBH from Nigeria, for example, had shown increment in high-density lipoprotein (HDL) cholesterol level, while hyperglycemia, triglycerides (TGs), very low-density lipoprotein (VLDL) cholesterol, non-HDL cholesterol levels, coronary risk index (CRI), and cardiovascular risk index (CVRI) were reduced in alloxan-induced male diabetic Wistar rats [61]. Pretreatment of EBH known as Gelam honey produced by Apis dorsata on pancreatic hamster cells has been reported to modify the inflammation-induced insulin signalling pathways [62]. Promising antihyperglycemic effects of EBH in the diabetic rabbit model has been reported as blood glucose levels, and other related parameters were significantly reduced in this study [63].…”