GDSL esterase/lipases OsGELP34 and OsGELP110/OsGELP115 are essential for rice pollen development

Zhang, Huihui; Wang, Menglong; Li, Yiqi; Wang, Yan; Chang, Zhenyi; Ni, Haoling; Chen, Zhufeng; Wu, Jianxin; Xu, Chunjue; Deng, Xing Wang; Tang, Xiaoyan

doi:10.1111/jipb.12919

Cited by 36 publications

(21 citation statements)

References 48 publications

(104 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, by integrative comparison among the 52 mutant bulks, it is much easier to clean the background SNPs and resolve the problems caused by the Illumina Hiseq platforms. Using this method, we have identified a total of 17,397 EMS-induced SNPs in 52 mutant bulks, including the SNPs responsible for the mutant phenotypes (Chang et al, 2016a(Chang et al, ,b, 2018Yan et al, 2017;Pan et al, 2020;Yuan et al, 2020;Zhang et al, 2020). The comparison of these SNPs with the re-sequencing data of a wild-type HHZ plant also verified the mutations.…”

Section: Discussionmentioning

confidence: 91%

The Genome-Wide EMS Mutagenesis Bias Correlates With Sequence Context and Chromatin Structure in Rice

et al. 2021

Self Cite

View full text Add to dashboard Cite

Ethyl methanesulfonate (EMS) is a chemical mutagen believed to mainly induce G/C to A/T transitions randomly in plant genomes. However, mutant screening for phenotypes often gets multiple alleles for one gene but no mutant for other genes. We investigated the potential EMS mutagenesis bias and the possible correlations with sequence context and chromatin structure using the whole genome resequencing data collected from 52 rice EMS mutants. We defined the EMS-induced single nucleotide polymorphic sites (SNPs) and explored the genomic factors associated with EMS mutagenesis bias. Compared with natural SNPs presented in the Rice3K project, EMS showed a preference on G/C sites with flanking sequences also higher in GC contents. The composition of local dinucleotides and trinucleotides was also associated with the efficiency of EMS mutagenesis. The biased distribution of EMS-induced SNPs was positively correlated with CpG numbers, transposable element contents, and repressive epigenetic markers but negatively with gene expression, the euchromatin marker DNase I hypersensitive sites, and active epigenetic markers, suggesting that sequence context and chromatin structure might correlate with the efficiency of EMS mutagenesis. Exploring the genome-wide features of EMS mutagenesis and correlations with epigenetic modifications will help in the understanding of DNA repair mechanism.

show abstract

Section: Discussionmentioning

confidence: 91%

The Genome-Wide EMS Mutagenesis Bias Correlates With Sequence Context and Chromatin Structure in Rice

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…CWPs in the functional class have been reported to be related to lipid metabolism [ 79 – 83 ]. LTPs are required for lipid export to the cell surface, and they are closely associated with cutin and wax formation [ 79 ].…”

Section: Discussionmentioning

confidence: 99%

“…One LTP has been found to be involved in cell wall extension by interacting with the cellulose/xyloglucan network [ 80 ]. GDSLs, a recently discovered subclass of lipolytic enzymes, possess multifunctional properties, and play important roles not only in the formation of surface cutin and epi-cuticular wax [ 81 ], but also in the tolerance to biotic and abiotic stresses [ 82 , 83 ]. In summary, numerous LTPs and GDSLs might play important roles in cuticle assembly during the growth and development of C. sinensis leaf.…”

Section: Discussionmentioning

confidence: 99%

Investigation of cell wall proteins of C. sinensis leaves by combining cell wall proteomics and N-glycoproteomics

Cao

et al. 2021

BMC Plant Biol

View full text Add to dashboard Cite

Background C. sinensis is an important economic crop with fluoride over-accumulation in its leaves, which poses a serious threat to human health due to its leaf consumption as tea. Recently, our study has indicated that cell wall proteins (CWPs) probably play a vital role in fluoride accumulation/detoxification in C. sinensis. However, there has been a lack in CWP identification and characterization up to now. This study is aimed to characterize cell wall proteome of C. sinensis leaves and to develop more CWPs related to stress response. A strategy of combined cell wall proteomics and N-glycoproteomics was employed to investigate CWPs. CWPs were extracted by sequential salt buffers, while N-glycoproteins were enriched by hydrophilic interaction chromatography method using C. sinensis leaves as a material. Afterwards all the proteins were subjected to UPLC-MS/MS analysis. Results A total of 501 CWPs and 195 CWPs were identified respectively by cell wall proteomics and N-glycoproteomics profiling with 118 CWPs in common. Notably, N-glycoproteomics is a feasible method for CWP identification, and it can enhance CWP coverage. Among identified CWPs, proteins acting on cell wall polysaccharides constitute the largest functional class, most of which might be involved in cell wall structure remodeling. The second largest functional class mainly encompass various proteases related to CWP turnover and maturation. Oxidoreductases represent the third largest functional class, most of which (especially Class III peroxidases) participate in defense response. As expected, identified CWPs are mainly related to plant cell wall formation and defense response. Conclusion This was the first large-scale investigation of CWPs in C. sinensis through cell wall proteomics and N-glycoproteomics. Our results not only provide a database for further research on CWPs, but also an insight into cell wall formation and defense response in C. sinensis.

show abstract

“…A LTP was also suggested to be involved in cell wall extension by interacting with the cellulose/xyloglucan network [83]. GDSLs, a newly discovered subclass of lipolytic enzymes, possess multifunctional properties which are assumed to play important roles not only in the formation of surface cutin and epi-cuticular wax [84], but also function in tolerance to biotic and abiotic stresses [85,86]. PAPs, might associated with the degradation of xyloglucan and oligosaccharides via dephosphorylating CWPs like alpha xylosidase and beta glucosidase [87].…”

Section: Possible Roles Of Identi Ed Cwpsmentioning

confidence: 99%

A Survey on Cell wall Proteins of C. Sinensis Leaf by Combining Cell Wall Proteomic and N-Glycoproteomic Strategy

liu

Cao

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Camellia sinensis is an important economic crop with fluoride over-accumulation in the leaves, which pose a serious threaten to human health due to its leave being used for making tea. Recently, our study found that cell wall proteins (CWPs) probably play a vital role in fluoride accumulation/detoxification in C. sinensis. However, CWPs identification and characterization were lacking up to now in C. sinensis. Herein, we aimed at characterizing cell wall proteome of C. sinensis leaves, to develop more CWPs related to stress response. A strategy of combined cell wall proteome and N-glycoproteome were employed to investigate CWPs. CWPs were extracted by sequential salt buffers, while N-glycoproteins were enriched by hydrophilic interaction chromatography method using C. sinensis leaves as a material, afterwards all proteins were subjected to qualitative analysis via UPLC-MS/MS.Results: 501 and 195 CWPs were identified by cell wall proteomic and N-glycoproteomics profiling, respectively, with 118 CWPs being in common. Notably, N-glycoproteome is a feasible method for CWPs identification and consequently enhance CWP coverage. Among identified CWPs, proteins acting on cell wall polysaccharides constitute the largest functional group with most of them possibly being involved in the remodeling of cell wall structure. The second abundant group encompass mainly various proteases, being considered to be related to CWPs turnover and maturation. Oxidoreductases represent the third abundance with most of them especially Class III peroxidases being known to be implicated in defense response. As expected, identified CWPs emphasized on plant cell wall formation and defense response.Conclusion: This was the first large scale survey of CWPs by cell wall proteome and N-glycoproteome in C. sinensis. The results not only provides a database that will aid deep research on CWPs, but also improve the understanding underlying cell wall formation and defense response in this important economic specie.

show abstract

GDSL esterase/lipases OsGELP34 and OsGELP110/OsGELP115 are essential for rice pollen development

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 36 publications

References 48 publications

The Genome-Wide EMS Mutagenesis Bias Correlates With Sequence Context and Chromatin Structure in Rice

The Genome-Wide EMS Mutagenesis Bias Correlates With Sequence Context and Chromatin Structure in Rice

Investigation of cell wall proteins of C. sinensis leaves by combining cell wall proteomics and N-glycoproteomics

A Survey on Cell wall Proteins of C. Sinensis Leaf by Combining Cell Wall Proteomic and N-Glycoproteomic Strategy

Contact Info

Product

Resources

About