gcpA (stm1987) is critical for cellulose production and biofilm formation on polystyrene surface by Salmonella enterica serovar Weltevreden in both high and low nutrient medium

Bhowmick, Patit Paban; Devegowda, Devananda; Ruwandeepika, H. A. D.; Fuchs, Thilo M.; Srikumar, Shabarinath; Karunasagar, Iddya; Karunasagar, Indrani

doi:10.1016/j.micpath.2010.12.002

Cited by 31 publications

(27 citation statements)

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“…Only one strain isolated from human feces (H43) did not present adrA . The obtained results were consistent with Halatsi et al [ 57 ] and Bhowmick et al [ 58 ], who detected the genes described above not only in S. enterica ser. Enteritidis, but also in other Salmonella serovars.…”

Section: Discussionsupporting

confidence: 92%

Antimicrobial Resistance and Biofilm Formation Capacity of Salmonella enterica Serovar Enteritidis Strains Isolated from Poultry and Humans in Poland

et al. 2020

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Salmonella enterica ser. Enteritidis (S. enterica ser. Enteritidis) is the most frequently detected serovar in human salmonellosis, and its ability to produce a biofilm and the risk of transmission from animals and food of animal origin to humans are significant. The main aim of the present work was to compare S. enterica ser. Enteritidis strains isolated from poultry and human feces in terms of resistance profiles, prevalence of selected resistance genes, and their potential for biofilm formation, by assessing their biofilm growth intensity, the prevalence and expression of selected genes associated with this phenomenon, and the correlation between increased antimicrobial resistance and biofilm formation ability of the two tested groups of S. enterica ser. Enteritidis. This study showed a difference in antimicrobial resistance (minimal inhibitory concentration value) between S. enterica ser. Enteritidis groups; however, the majority of multidrug-resistant (MDR) strains were isolated from poultry (environmental samples from chicken broilers, turkey broilers, and laying hens). Differences in the prevalence of resistance genes were observed; the most common gene among poultry strains was floR, and that among strains from humans was blaTEM. S. enterica ser. Enteritidis strains isolated from poultry under the tested incubation conditions exhibited better biofilm growth than strains isolated from humans. A higher level of gene expression associated with the production of cellulose was only detected in the S48 strain isolated from poultry. On the other hand, increased expression of genes associated with quorum sensing was observed in two strains isolated from poultry farms and one strain isolated from human feces.

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Section: Discussionsupporting

confidence: 92%

Antimicrobial Resistance and Biofilm Formation Capacity of Salmonella enterica Serovar Enteritidis Strains Isolated from Poultry and Humans in Poland

et al. 2020

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“…However, transcription of the two putative cellulose synthase genes identified here in the K. pneumoniae genome (bcsA1 and bcsA2) did not change in a yfiR mutant background. This result indicates that the effect of c-di-GMP on cellulose production most probably occurs via the PilZ domains in both predicted Bcs proteins, as has been shown in Gluconoacetobacter xylinus and several enterobacteria (Amikam & Galperin, 2006;Bhowmick et al, 2011;Raterman et al, 2013;Ryjenkov et al, 2006). Regulation of cellulose production by c-di-GMP has been studied extensively at the molecular level, and recent mechanistic insight has been obtained regarding enzyme activity based on analysis of the crystal structure of the Rhodobacter sphaeroides c-di-GMP-activated BcsA-BcsB complex (Morgan et al, 2014).…”

Section: Discussionsupporting

confidence: 66%

Klebsiella pneumoniae yfiRNB operon affects biofilm formation, polysaccharide production and drug susceptibility

et al. 2014

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Klebsiella pneumoniae is an opportunistic pathogen important in hospital-acquired infections, which are complicated by the rise of drug-resistant strains and the capacity of cells to adhere to surfaces and form biofilms. In this work, we carried out an analysis of the genes in the K. pneumoniae yfiRNB operon, previously implicated in biofilm formation. The results indicated that in addition to the previously reported effect on type 3 fimbriae expression, this operon also affected biofilm formation due to changes in cellulose as part of the extracellular matrix. Deletion of yfiR resulted in enhanced biofilm formation and an altered colony phenotype indicative of cellulose overproduction when grown on solid indicator media. Extraction of polysaccharides and treatment with cellulase were consistent with the presence of cellulose in biofilms. The enhanced cellulose production did not, however, correlate with virulence as assessed using a Caenorhabditis elegans assay. In addition, cells bearing mutations in genes of the yfiRNB operon varied with respect to the WT control in terms of susceptibility to the antibiotics amikacin, ciprofloxacin, imipenem and meropenem. These results indicated that the yfiRNB operon is implicated in the production of exopolysaccharides that alter cell surface characteristics and the capacity to form biofilms -a phenotype that does not necessarily correlate with properties related with survival, such as resistance to antibiotics.

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“…OmpR transcriptionally activates the csgDEFG operon; CsgD in turn activates transcription of the csgBAC operon, encoding the curli structural proteins which enable initial attachment of bacteria to surfaces (Prigent-Combaret et al, 2001; Ogasawara et al, 2010; Brombacher et al, 2003). In addition, CsgD also activates transcription of adrA , encoding a putative diguanylate cyclase which is predicted to generate c-di-GMP and thus activate cellulose production (Bhowmick et al, 2011). The ompR234 mutation carried in strains PHL628 and PHL644 comprises a point mutation (L43R) located within the receiver domain, which enhances activation of csgDEFG (Prigent-Combaret et al, 2001; Prigent-Combaret et al, 1999; Vidal et al 1998).…”

Section: Discussionmentioning

confidence: 99%

Optimisation of engineered Escherichia coli biofilms for enzymatic biosynthesis of l-halotryptophans

et al. 2013

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Engineered biofilms comprising a single recombinant species have demonstrated remarkable activity as novel biocatalysts for a range of applications. In this work, we focused on the biotransformation of 5-haloindole into 5-halotryptophan, a pharmaceutical intermediate, using Escherichia coli expressing a recombinant tryptophan synthase enzyme encoded by plasmid pSTB7. To optimise the reaction we compared two E. coli K-12 strains (MC4100 and MG1655) and their ompR234 mutants, which overproduce the adhesin curli (PHL644 and PHL628). The ompR234 mutation increased the quantity of biofilm in both MG1655 and MC4100 backgrounds. In all cases, no conversion of 5-haloindoles was observed using cells without the pSTB7 plasmid. Engineered biofilms of strains PHL628 pSTB7 and PHL644 pSTB7 generated more 5-halotryptophan than their corresponding planktonic cells. Flow cytometry revealed that the vast majority of cells were alive after 24 hour biotransformation reactions, both in planktonic and biofilm forms, suggesting that cell viability was not a major factor in the greater performance of biofilm reactions. Monitoring 5-haloindole depletion, 5-halotryptophan synthesis and the percentage conversion of the biotransformation reaction suggested that there were inherent differences between strains MG1655 and MC4100, and between planktonic and biofilm cells, in terms of tryptophan and indole metabolism and transport. The study has reinforced the need to thoroughly investigate bacterial physiology and make informed strain selections when developing biotransformation reactions.

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gcpA (stm1987) is critical for cellulose production and biofilm formation on polystyrene surface by Salmonella enterica serovar Weltevreden in both high and low nutrient medium

Cited by 31 publications

References 50 publications

Antimicrobial Resistance and Biofilm Formation Capacity of Salmonella enterica Serovar Enteritidis Strains Isolated from Poultry and Humans in Poland

Antimicrobial Resistance and Biofilm Formation Capacity of Salmonella enterica Serovar Enteritidis Strains Isolated from Poultry and Humans in Poland

Klebsiella pneumoniae yfiRNB operon affects biofilm formation, polysaccharide production and drug susceptibility

Optimisation of engineered Escherichia coli biofilms for enzymatic biosynthesis of l-halotryptophans

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