GC–MS based metabolomics used for the identification of cancer volatile organic compounds as biomarkers

Lubes, Giuseppe; Goodarzi, Mohammad

doi:10.1016/j.jpba.2017.07.013

Cited by 112 publications

(54 citation statements)

References 72 publications

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“…The second is matrix-assisted laser ionization mass spectrometry (MALDI) [ 50 ], which is suitable for the blood lipids and highly hydrophobic and nonpolar lipids, (phospholipids, for example). The third method is GC/LC–MS [ 51 ], which needs to transform lipid molecules into a gaseous state by derivatization before they can be studied. The most common application is to derivatize a lipid with methanol-forming FA methyl ester (FAME) for downstream analysis.…”

Section: Research Progress Of Lipidomicsmentioning

confidence: 99%

The Function and Mechanism of Lipid Molecules and Their Roles in The Diagnosis and Prognosis of Breast Cancer

et al. 2020

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Lipids are essential components of cell structure and play important roles in signal transduction between cells and body metabolism. With the continuous development and innovation of lipidomics technology, many studies have shown that the relationship between lipids and cancer is steadily increasing, involving cancer occurrence, proliferation, migration, and apoptosis. Breast cancer has seriously affected the safety and quality of life of human beings worldwide and has become a significant public health problem in modern society, with an especially high incidence among women. Therefore, the issue has inspired scientific researchers to study the link between lipids and breast cancer. This article reviews the research progress of lipidomics, the biological characteristics of lipid molecules, and the relationship between some lipids and cancer drug resistance. Furthermore, this work summarizes the lipid molecules related to breast cancer diagnosis and prognosis, and then it clarifies their impact on the occurrence and development of breast cancer The discussion revolves around the current research hotspot long-chain non-coding RNAs (lncRNAs), summarizes and explains their impact on tumor lipid metabolism, and provides more scientific basis for future cancer research studies.

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Section: Research Progress Of Lipidomicsmentioning

confidence: 99%

The Function and Mechanism of Lipid Molecules and Their Roles in The Diagnosis and Prognosis of Breast Cancer

et al. 2020

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“…The linear viscoelastic region (LVE) was determined via frequency sweep between 0.1 and 100 Hz and amplitude sweep at a constant angular frequency of 0.1 rad/s and deformation (γ) between 0.1 to 100. The viscoelastic moduli G' (i.e., elastic modulus) and G" (i.e., viscous modulus) were determined via strain-controlled oscillation using a Physica MCR 301 rheometer (Anton Paar) with a CP-50-1 measurement system (cone-plate geometry) at RT (25 ± 0.5 • C) and 37 ± 0.5 • C [42]. The loss factor tanδ, which describes the ratio of viscous and elastic behavior, was calculated according to G"/G' for each measurement point.…”

Section: Preparation Of the Investigated Formulationsmentioning

confidence: 99%

“…The most frequently used in vitro methods include rheological techniques, surface plasmon resonance, isothermal titration calorimetry, tensile strength test, shear strength test, peel strength test, and contact angle measurements performed on specific surfaces [28,34,41]. In addition, mucoadhesion can be tested ex vivo using excised tissues [38,39,41,42]. Thereby, the everted gut sac technique, atomic force microscopy and the falling liquid film method are the most frequently used techniques to study mucoadhesion along the oro-gastrointestinal tract [43][44][45].…”

Section: Introductionmentioning

confidence: 99%

Investigations to Evaluate Gastric Mucoadhesion of an Organic Product to Ameliorate Gastritis

et al. 2020

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Gastritis is an inflammatory disease leading to abdominal pain, nausea, and diarrhea. While therapy depends on etiology, adhesive agents protecting the gastric tissue represent a promising treatment option. Caricol®-Gastro is an organic product that significantly decreased gastritic abdominal pain in a recent clinical study. To investigate whether this beneficial effect can be attributed to the formation of a protective layer covering the gastric mucosa after oral application, several methods were used to determine adhesion. These include macro-rheological measurements and gastric mucin interactions, which were correlated to network formation, examined by Cryo-scanning electron microscopy technique, wettability via sessile drop method on human gastric adenocarcinoma cell layers, and ex vivo adhesion studies on gastric porcine tissue with the falling liquid film technique considering physiological conditions and Franz diffusion cells for quantification. The results showed that Caricol®-Gastro formed a stable viscoelastic network with shear thinning properties. It exhibited high wettability and spreadability and adhered to the excised gastric mucosa. We found that oat flour, as the main ingredient of Caricol®-Gastro, supports the gel network regarding viscoelasticity and, to a lesser extent, adhesion in a concentration dependent manner. Moreover, our data highlight that a variety of coordinated methods are required to investigate gastric adhesion.

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“…Exhaled human breath consists of several different compounds, including volatile organic compounds (VOCs), which are continuously generated in the human body and are partially emitted via exhaled breath, through the skin, and by urine and feces [1][2][3]. VOCs are mainly in the ppm-ppt (part per million to part per trillion) range, thus, laboratory methods are used for their detection in breath, such as GC-MS (gas chromatography-mass spectrometry) [4,5], PTR-MS (proton transfer reaction-mass spectrometry) [6,7], IMS-MS (ion-mobility spectrometry-mass spectrometry) [8,9], and SIFT-MS (selected ion flow tube-mass spectrometry) [10,11]. Over the last 40 years, almost 3500 different VOCs have been detected in the human breath [12], and a single breath consists of around 500 various VOCs [13].…”

Section: Introductionmentioning

confidence: 99%

A Prototype of a Portable Gas Analyzer for Exhaled Acetone Detection

Sorocki¹,

Rydosz²

2019

Applied Sciences

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The paper presents the development of a portable gas analyzer prototype for exhaled acetone detection, employing an application-suited gas sensor array and 3D printing technology. The device provides the functionality to monitor exhaled acetone levels, which could be used as a potential tool for non-invasive diabetes monitoring. The relationship between exhaled acetone concentrations and glucose in blood is confirmed in the literature, including research carried out by the authors. The design process is presented including a general consideration for the sensor array construction, which is the core for sensing gases, as well as requirements for the measurement chamber it is to be placed in. Moreover, the mechanical design of the 3D-printed housing is discussed to ensure the ergonomics of use as a hand-held device while keeping the hardware integrity. Also, the processing hardware is discussed to provide sufficient computing power to handle the stand-alone operation while being energy efficient, enabling long battery-powered operation. Finally, calibration and measurement, as well as the analyzer operation, are shown, validating the proposed class of exhaled acetone-detection capable meters.

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GC–MS based metabolomics used for the identification of cancer volatile organic compounds as biomarkers

Cited by 112 publications

References 72 publications

The Function and Mechanism of Lipid Molecules and Their Roles in The Diagnosis and Prognosis of Breast Cancer

The Function and Mechanism of Lipid Molecules and Their Roles in The Diagnosis and Prognosis of Breast Cancer

Investigations to Evaluate Gastric Mucoadhesion of an Organic Product to Ameliorate Gastritis

A Prototype of a Portable Gas Analyzer for Exhaled Acetone Detection

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