GC–MS-based metabolomics study of the responses to arachidonic acid in Blakeslea trispora

Hu, Xianmei; Li, Hao; Tang, Pingwah; Sun, Jie; Yuan, Qipeng; Li, Chunfang

doi:10.1016/j.fgb.2013.06.002

Cited by 31 publications

(11 citation statements)

References 30 publications

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“…In Physalis peruviana we observed an evident down‐regulation of malic acid production; therefore, we propose that the TCA cycle was down‐regulated to enable more acetyl‐CoA to enter the carotenoid synthesis pathway. This is consistent with a previous study in which a decrease in malic acid increased β‐carotene production in the yeast Blakeslea trispora (Hu et al., ).…”

Section: Resultssupporting

confidence: 93%

Metabolomic Profiling of Carotenoid Constituents in Physalis peruviana During Different Growth Stages by LC‐MS/MS Technology

Chen

Zheng

2019

Journal of Food Science

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With the current ongoing changes in global food demands, natural carotenoids are preferred by consumers and are gaining attention among food scientists and producers alike. Metabolomic profiling of carotenoid constituents in Physalis peruviana during distinct on-tree growth stages was performed with liquid chromatography-tandem mass spectrometry (LC-MS/MS) technology. The results show that the β rings of β-carotene are hydroxylated with great efficiency, and there is a continual synthesis of zeaxanthin at half-ripe and full-ripe stages, which is confirmed by relating the zeaxanthin content to that of its precursor (β-carotene). Lutein was, in terms of mass intensity, the most abundant carotenoid constituent (64.61 µg/g at the half-ripe stage) observed in this study. In addition, γ -carotene, which is rare in dietary fruits and vegetables, was detected in the mature and breaker stages, albeit at a relatively low level. The results suggest that when we consider the variation in carotenoid content during different growth stages, Physalis peruviana can be considered a good source of natural carotenoids.

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Section: Resultssupporting

confidence: 93%

Metabolomic Profiling of Carotenoid Constituents in Physalis peruviana During Different Growth Stages by LC‐MS/MS Technology

Chen

Zheng

2019

Journal of Food Science

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“…The analysis was validated by default sevenfold internal cross-validation based on the R 2 X and Q 2 values which are the goodness of fit and goodness of prediction, respectively. The PCA, which is an unsupervised analysis, was conducted in order to determine an overview of the possible differences or similarities among the groups of samples based on the observation in the score plots [ 12 ].…”

Section: Methodsmentioning

confidence: 99%

Stability Study of Algerian Nigella sativa Seeds Stored under Different Conditions

Bustamam

Hadithon

Mediani

et al. 2017

Journal of Analytical Methods in Chemistry

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In a study to determine the stability of the main volatile constituents of Nigella sativa seeds stored under several conditions, eight storage conditions were established, based on the ecological abiotic effects of air, heat, and light. Six replicates each were prepared and analyzed with Headspace-Gas Chromatography-Mass Spectrometry (HS-GC-MS) for three time points at the initial (1st day (0)), 14th (1), and 28th (2) day of storage. A targeted multivariate analysis of Principal Component Analysis revealed that the stability of the main volatile constituents of the whole seeds was better than that of the ground seeds. Exposed seeds, whole or ground, were observed to experience higher decrement of the volatile composition. These ecofactors of air, heat, and light are suggested to be directly responsible for the loss of volatiles in N. sativa seeds, particularly of the ground seeds.

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“…Cells were sampled every 24 h until 120 h during fermentation. The collected cell was quenched and extracted according to the methods reported by Li et al and Hu et al with moderate modifications . The sample was quickly mixed with cold 60% methanol (−40 °C, v/v) to capture the metabolism of cells instantaneously and then centrifuged at 5000 g and −4 °C for 3 min.…”

Section: Methodsmentioning

confidence: 99%

“…For GC‐MS analysis, derivatization of samples was performed according to the method of Hu et al with moderate modifications . The 0.5 mL sample obtained above and 50 μL internal standard (adonitol in water, 1 mg mL −1 ) were dried in a vacuum centrifuge dryer.…”

Section: Methodsmentioning

confidence: 99%

Metabolomics analysis reveals 6‐benzylaminopurine as a stimulator for improving lipid and DHA accumulation of Aurantiochytriumsp.

Sun

Zheng

et al. 2016

J of Chemical Tech & Biotech

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BACKGROUNDPhytohormones are chemical messengers that have a positive effect on biodiesel production of microalgae at low concentrations. However, the effect of phytohormone 6‐benzylaminopurine on lipid and docosahexaenoic acid (DHA) production in marine DHA‐producer Aurantiochytrium has never been reported. In this study, a GC‐MS‐based metabolomics method combined with a multivariate analysis is applied to reveal the metabolic mechanism of 6‐benzylaminopurine enhancing production of lipid and DHA in Aurantiochytrium sp.YLH70.RESULTSIn total, 71 metabolites were identified by GC‐MS. The PCA model revealed that 76.9% of metabolite variation was related to 6‐benzylaminopurine treatment, and overall metabolomics profiles between the 6‐benzylaminopurine and control groups were clearly discriminated. Forty‐six metabolites identified by the PLS‐DA model were responsible for responding to 6‐benzylaminopurine. Metabolic analysis showed that 6‐benzylaminopurine could accelerate the rate of utilization of glucose in Aurantiochytrium sp. YLH70, and the metabolic flux from glycolysis, TCA cycle and mevalonate pathway to fatty acids biosynthesis was promoted. Moreover, the anti‐stress mechanism in Aurantiochytrium sp.YLH70 might be induced by 6‐benzylaminopurine.CONCLUSIONMetabolomics is a suitable tool to discover the metabolic mechanism for improving lipid and DHA accumulation in a microorganism. 6‐benzylaminopurine has the potential to stimulate lipid and DHA production of Aurantiochytrium sp.YLH70 for industrial purposes. © 2015 The Authors. Journal of Chemical Technology & Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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GC–MS-based metabolomics study of the responses to arachidonic acid in Blakeslea trispora

Cited by 31 publications

References 30 publications

Metabolomic Profiling of Carotenoid Constituents in Physalis peruviana During Different Growth Stages by LC‐MS/MS Technology

Metabolomic Profiling of Carotenoid Constituents in Physalis peruviana During Different Growth Stages by LC‐MS/MS Technology

Stability Study of Algerian Nigella sativa Seeds Stored under Different Conditions

Metabolomics analysis reveals 6‐benzylaminopurine as a stimulator for improving lipid and DHA accumulation of Aurantiochytriumsp.

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