GC-MS-Based Analysis of Methanol: Chloroform-extracted Fatty Acids from Plant Tissues

Patel, Manish Kumar; Das, Shubhashis; Thakur, Jitendra K.

doi:10.21769/bioprotoc.3014

Cited by 10 publications

(8 citation statements)

References 14 publications

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“…Nitrite is a limiting factor for NO production (Planchet et al , 2005). Our results suggest that Kabuli has reduced levels of nitrite (see Supplementary Fig.…”

Section: Discussionmentioning

confidence: 99%

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Nitric oxide accelerates germination via the regulation of respiration in chickpea

Pandey

Kumari

Shree

et al. 2019

Journal of Experimental Botany

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Seed germination is crucial for the plant life cycle. We investigated the role of nitric oxide (NO) in two chickpea varieties that differ in germination capacity: Kabuli, which has a low rate of germination and germinates slowly, and Desi, which shows improved germination properties. Desi produced more NO than Kabuli and had lower respiratory rates. As a result of the high respiration rates, Kabuli had higher levels of reactive oxygen species (ROS). Treatment with the NO donor S-nitroso-N-acetyl-D,L-penicillamine (SNAP) reduced respiration in Kabuli and decreased ROS levels, resulting in accelerated germination rates. These findings suggest that NO plays a key role in the germination of Kabuli. SNAP increased the levels of transcripts encoding enzymes involved in carbohydrate metabolism and the cell cycle. Moreover, the levels of amino acids and organic acids were increased in Kabuli as a result of SNAP treatment. 1H-nuclear magnetic resonance analysis revealed that Kabuli has a higher capacity for glucose oxidation than Desi. An observed SNAP-induced increase in 13C incorporation into soluble alanine may result from enhanced oxidation of exogenous [13C]glucose via glycolysis and the pentose phosphate pathway. A homozygous hybrid that originated from a recombinant inbred line population of a cross between Desi and Kabuli germinated faster and had increased NO levels and a reduced accumulation of ROS compared with Kabuli. Taken together, these findings demonstrate the importance of NO in chickpea germination via the control of respiration and ROS accumulation.

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“…Nitrite is a limiting factor for NO production (Planchet et al , 2005). Our results suggest that Kabuli has reduced levels of nitrite (see Supplementary Fig.…”

Section: Discussionmentioning

confidence: 99%

“…For a standard, 0.1–10 µM KNO 2 was used. NR activity was measured according to (Planchet et al , 2005).…”

Section: Methodsmentioning

confidence: 99%

Nitric oxide accelerates germination via the regulation of respiration in chickpea

Pandey

Kumari

Shree

et al. 2019

Journal of Experimental Botany

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“…The extraction of FAs was performed as previously described by Patel et al. (2018) with some modifications. Briefly, Col‐0 and kcs1‐5 roots were grown in Murashige and Skoog medium for 10 days and transferred to a control Murashige and Skoog medium or Murashige and Skoog medium with 300 n m cafenstrole for 3 days.…”

Section: Methodsmentioning

confidence: 99%

A very long chain fatty acid responsive transcription factor, MYB93, regulates lateral root development in Arabidopsis

et al. 2023

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SUMMARYLateral roots (LRs) are critical to root system architecture development in plants. Although the molecular mechanisms by which auxin regulates LR development have been extensively studied, several additional regulatory systems are hypothesized to be involved. Recently, the regulatory role of very long chain fatty acids (VLCFAs) has been shown in LR development. Our analysis showed that LTPG1 and LTPG2, transporters of VLCFAs, are specifically expressed in the developing LR primordium (LRP), while the number of LRs is reduced in the ltpg1/ltpg2 double mutant. Moreover, late LRP development was hindered when the VLCFA levels were reduced by the VLCFA synthesis enzyme mutant, kcs1‐5. However, the details of the regulatory mechanisms of LR development controlled by VLCFAs remain unknown. In this study, we propose a novel method to analyze the LRP development stages with high temporal resolution using a deep neural network and identify a VLCFA‐responsive transcription factor, MYB93, via transcriptome analysis of kcs1‐5. MYB93 showed a carbon chain length‐specific expression response following treatment of VLCFAs. Furthermore, myb93 transcriptome analysis suggested that MYB93 regulated the expression of cell wall organization genes. In addition, we also found that LTPG1 and LTPG2 are involved in LR development through the formation of root cap cuticle, which is different from transcriptional regulation by VLCFAs. Our results suggest that VLCFA is a regulator of LRP development through transcription factor‐mediated regulation of gene expression and the transportation of VLCFAs is also involved in LR development through root cap cuticle formation.

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“…The extraction of fatty acids was performed according to described in Patel et al (2018) with some modifications. Briefly, Col-0 roots grown in MS medium for 10 days and transferred to e control MS medium or MS medium with 300 nM cafenstrole 3 d. ksc1-5 , ltpg1 , and ltpg2 roots were grown in MS medium for 13 d. Root samples (500 mg) of each were corrected, and frozen, and ground.…”

Section: Methodsmentioning

confidence: 99%

A very long chain fatty acid responsive transcription factor, MYB93, regulates lateral root development in Arabidopsis

Uemura

Kimura

Ohta

et al. 2022

Preprint

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Lateral roots (LRs) are critical to rhizosphere development in plants. Although the molecular mechanisms by which auxin regulates LR development has been studied extensively, many additional regulatory systems are thought to be involved. Based on the expression analysis of LTPG1 and 2, we found that they were specifically expressed at the developing LR primordium (LRP), and the number of LRs were reduced in these mutants. Because LTPG is a protein that transports Very Long Chain Fatty Acids (VLCFAs), we hypothesized that VLCFAs regulated LR development. We revealed that late LRP development was stunted when VLCFA levels were reduced in the synthetic mutant, kcs1-5. In this study, we established a method to analyze the LRP development stages with high temporal resolution using a deep neural network. We identified a VLCFA responsible transcription factor, MYB93, from transcriptome analysis of kcs1-5. Interestingly, MYB93 showed a carbon chain length-specific expression response after treatment of VLCFA. Furthermore, myb93 transcriptome analysis suggested that MYB93 regulated the expression of cell wall organization genes. Our results indicated that VLCFA is a regulator of LRP development through transcription factor-mediated regulation of gene expression.

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GC-MS-Based Analysis of Methanol: Chloroform-extracted Fatty Acids from Plant Tissues

Cited by 10 publications

References 14 publications

Nitric oxide accelerates germination via the regulation of respiration in chickpea

Nitric oxide accelerates germination via the regulation of respiration in chickpea

A very long chain fatty acid responsive transcription factor, MYB93, regulates lateral root development in Arabidopsis

A very long chain fatty acid responsive transcription factor, MYB93, regulates lateral root development in Arabidopsis

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