GC-MS Analysis of Young Leaves of Allophylus cobbe (L.) Raeusch. and Allophylus serratus (Roxb.) Kurz.

Abstract

Background: Allophylus cobbe(L.) Raeusch. and Allophylus serratus (Roxb.)Kurz. (Sapindaceae) are medicinal plants traditionally used to cure bone fractures and other ailments in India. Objective: Present work was aimed at the identification of phytochemical compounds from young leaves of A. cobbe and A. serratus by applying gas chromatography mass spectrometry. Method: GC-MS technique was used for analysis of compounds. Results: It indicates 11 compounds from A. serratus and seven compounds from A. cobbe having mixture of terpenoids, alkanes and fatty acids. The major compounds found in A. cobbe were 1,1-diethoxy ethane (82.97%) (RT: 3.192min), phytol (7.07%) (RT: 43.690 min) and hexanoic acid (3.67%) (RT: 29.576 min) while in A. serratus 3-methyl butanol (53.16%) (RT: 3.223 min), 2-propenoic acid, 2-(dimethylamino) ethyl ester (27.08%) (RT: 13.94 min) and diisocytyl phthalate (4.49%) (RT: 52.584 min) were found in higher quantity. Phytol was detected from both species. Phytol possesses anti-radical, anti-cancer, antibacterial and anti-inflammatory properties as well as used in artificial synthesis of vitamin E and vitamin K in cosmetics and fragrance industry. Conclusion:The abundant presence of phytol in leaves of Allophylus species (7.07% in A. cobbe and 2.60% in A. serratus) suggests that these species may prove helpful in prevention of degenerative diseases and application of these herbs in cosmetics which may prove a cost effective natural alternative to current synthetic cosmetics. Presence of industrially important chemicals like phytol, 1,1-diethoxy ethane, hexanoic acid, diisocytyl phthalate, 2-propeonic acid, 2-(dimethylamino) ethyl ester underlines industrial importance of these species which needs further research on this aspect to focus in detail.

Antimicrobial Activity and Chemical Composition Screening of Anacyclus pyrethrum Root

“…15 For the identification of chemical components, each sample was analysed by Shimadzu GCMS QP 2010 ULTRA equipped with RTX-5MS capillary column (30m * 0.25 mm; coating thickness 0.25 m). Analytical conditions were an injector temperature of 250 ºC; carrier gas Helium at 1.78 mL/min; injection mode: split, split ratio 10:1; volume injected: 1 L of sample in ethanol extract and oven temperature programmed from 40 ºC to 290 ºC at 4 ºC/min, pressure: 100 kPa, total flow: 13.7 mL/min.…”

Biochemical, Antioxidant Properties and Antimicrobial Activity of Steno-Endemic Origanum onites

“…The DPPH scavenging activities for the hydroethanolic extracts of Greek and common oregano were reported as 70.90% and 69.83%, respectively, with corresponding Trolox equivalent values of 252.10 and 242.43 µmol/g. In another study by Kaurinovic et al [ 66 ], the DPPH scavenging activity of various O. basilicum and O. vulgare extracts was investigated, and the IC 50 values for O. vulgare water and n-BuOH extracts were found to show stronger antioxidant effects than BHT. In comparison to the findings of Kaurinovic et al, our results indicate that OOEt possesses a more potent antioxidant capacity.…”

Identification of antidiabetic inhibitors from Allophylus villosus and Mycetia sinensis by targeting α-glucosidase and PPAR-γ: In-vitro, in-vivo, and computational evidence

“…N-hexane fractioned has skipped due to no peaks performed in GC analysis, which was conducted before GC–MS for all fractioned extracts of A. villosus and M. sinensis . GC–MS method was developed in-house ( Bharat et al, 2017 ) where Gas Chromatograph (Agilent, USA 8890) with an i.d. (30 m x 0.25 mm) and 0.5 um film thickness capillary column HP-5 ms (Agilent USA, Serial no: T562436H) was used at f scan mode (total) and split ratio 1:20 (split injector mode was), Mass Selective Detector (MSD) was 5977C single quadrupole, injector temperature 220 0 interface temperature 230 0 and the injection volume was 1ul in GC–MS.…”