GC–MS Analysis of the Essential Oils of Piper nigrum L. and Piper longum L.

Liu, Ling; Song, Guoxin; Hu, Yaoming

doi:10.1365/s10337-007-0408-2

Cited by 51 publications

(40 citation statements)

References 24 publications

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“…30 The GCMS analysis revealed the presence of piperidinone, hydrocinnamic acid, ethylhexahydro azepine, methyleugenol, hexadecanoic acid and caryophyllene oxide were identified in the methanolic extract of P. longum while octadecatrienoic acid, caryophyllene, caryophyllene oxide and hydrocinnamic acid were present in the chloroform fraction of P. longum. Similar compounds were isolated by Liu et al 23 from plants belonging to piperaceae family including spathulenol, piperitone, eugenol and caryophyllene. Mohammed et al 34 isolated piperidine, benzofuran, …”

Section: Structurally Similar Compoundssupporting

confidence: 70%

“…23 The potent extract/fraction was dissolved in methanol, filtered in 0.22-micron syringe filter and the compounds were separated on TSQ-2MS capillary column (30 m × 0.25 mm; i.e., 0.25 µm film). The column oven temperature was programmed from an initial temperature of 40 º C (3 min) to 160 º C at 8 º C min -1 , then to 300 º C at 15 º C min -1 with a final time of 10 min.…”

Section: Gas Chromatography-mass Spectrometry (Gc-ms)mentioning

confidence: 99%

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Anthelmintic Activity of Fruit Extract and Fractions of Piper longum L. In vitro

Koorse¹,

Samraj²,

John³

et al. 2018

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The present study was aimed to assess the in vitro ovicidal, larvicidal and adulticidal activity of methanolic extract and its fractions from fruits of Piper longum against strongyle ova, larvae and adult amphistomes respectively. The fruits of P. longum was identified and the accession number 006 was obtained. The phytochemical analysis revealed the presence of tannins, flavonoids, glycosides, phenolics, diterpenes and triterpenes in extract and fractions of P. longum. The extract and fractions were diluted serially in 6.25 per cent tween 80 to obtain concentrations of 500, 250, 125, 62.5, 31.25, 15.63, 7.81, 3.91 and 1.95 mg/mL. Ivermectin and thiabendazole at 10 µg/mL acted as positive controls and 6.25 per cent tween 80 as negative control. The methanolic extract was highly active against ova with IC 50 of 0.026 mg/mL. The n-hexane fraction was potent in inducing larval mortality with IC 50 of 1.383 mg/mL while chloroform fraction inhibited larval migration with IC 50 of 1.796 mg/mL. Amphistomes were highly sensitive for methanolic extract of P. longum which possessed IC 50 of 5.493 mg/mL Based on IC 50 values, the methanolic extract was found to be most potent while chloroform fraction was effective against ova, larvae and also adults. GCMS analysis of potent methanolic extract revealed the presence of piperidinone, hydrocinnamic acid, ethylhexahydro azepine, methyleugenol, hexadecanoic acid and caryophyllene oxide which may have contributed for the anthelmintic activity. The acute oral toxicity study revealed mild vascular changes in liver. From the present study, it can be concluded that chloroform fraction of P. longum possessed maximum broad spectrum anthelmintic activity comparable to controls.

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Section: Structurally Similar Compoundssupporting

confidence: 70%

Section: Gas Chromatography-mass Spectrometry (Gc-ms)mentioning

confidence: 99%

Anthelmintic Activity of Fruit Extract and Fractions of Piper longum L. In vitro

Koorse¹,

Samraj²,

John³

et al. 2018

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“…The components of the oil were identiWed by comparison of their mass spectra with those of the spectrometer database using the NIST147 mass spectral database. The identiWcation was conWrmed by comparison of Kovats indices (KI) [19,20,22,31] with those reported in the literature [24,[31][32][33][34][35][36]. The KI was set-up a standard mixture of C 8 -C 26 compounds under chromatographic conditions, consistent with those of the chromatographic conditions of the samples analyzed.…”

Section: ¡1mentioning

confidence: 99%

“…However, so far, the essential oils components in P. furcata Royle have not received much attention. Therefore, in the current work, for the Wrst time, microwave-assisted extraction (MAE) [10][11][12][13][14][15][16] followed by HS-SPME [17][18][19][20][21][22][23][24][25][26][27][28][29][30] and GC-MS was developed for the fast determination of volatile chemical compositions in the fresh aerial parts of P. furcata Royle. The experimental parameters were studied by orthogonal array design, and the precision of the method was also investigated.…”

Section: Introductionmentioning

confidence: 99%

Rapid determination of volatile composition from Polygala furcata Royle by MAE–HS-SPME followed by GC–MS

Zhou

Mao

Long

et al. 2010

Eur Food Res Technol

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Based on microwave-assisted extraction (MAE) and headspace solid-phase microextraction (HS-SPME) followed by gas chromatography mass spectrometry (GC-MS) was developed for the fast determination of volatile chemical compositions of Polygala furcata Royle, the experimental parameters of the MAE-HS-SPME were optimized by orthogonal experimental design. The results indicated that the optimum condition of the determination of the volatile compounds in P. furcata Royle. was achieved with the experimental parameters including microwave power of 400 W and irradiation time of 4 min, sample size 2.0 g. Under the optimal conditions, for the Wrst time, 52 volatile compounds were separated and identiWed from the fresh plants of P. furcata Royle. The highest content component of the 52 compounds was 2-hydroxybenzoic acid methyl ester (21.65%). The relative standard deviation values <7% showed that the method of MAE-HS-SPME followed by GC-MS has good precision. The experimental results demonstrated that it is a simple, time-saving and solvent-free method, and it is a potential analytic tool for the determination of the volatile compounds of the vegetations materials and other materials.

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“…Subsequently, the essential oils were 'spiked' using the combined chromatography technique [9]. Using the same protocol as before, each essential oil sample was injected with a mixture of standard hydrocarbon series of C 8 -C 21 . Location of each carbon series were identified based on the retention time of carbon series ran in the chromatogram earlier.…”

Section: Analyses Of the Essential Oilsmentioning

confidence: 99%

Anti-Inflammatory Activity of Selected Edible Herbs and Spices on Cultured Human Gingival Fibroblasts

Said¹

2013

TOPROCJ

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Abstract:The aims of the study were to determine the in vitro cytotoxicity and anti-inflammatory activities of common edible herbs and spices on cultured human gingival fibroblasts (HGFs). Piper betle L. (betel leaf), P.sarmentosum Roxb.(wild betel / kadok leaf), P.nigrum L. (black pepper seed), Eugenia caryophyllata L. (clove bud) and Cinnamomum zeylanicum Blume (cinnamon bark). Essential oils were extracted using steam distillation technique and analysed using gas chromatography (GC) and gas chromatography -mass spectrometry (GC-MS). The HGFs were exposed to essential oils at 5 -0.04 µg/mL in less than 1% dimethylsulfoxide and the number of viable cells was counted to assess cytotoxicity effect. Anti-inflammatory action was determined via the inhibitory action of Interleukin-6 (IL-6), a major proinflammatory cytokine in the periodontal tissue inflammation. Treatment of fibroblasts with essential oils resulted in > 70% cell viability. The oils from black pepper seed, clove bud and cinnamon bark showed dose-dependent inhibitory action on IL-6 on cultured bacterial Lipopolysaccharide (LPS)-induced human gingival fibroblasts. Of all the oils, cinnamon bark oil showed the most prominent action comparable to acetylsalicylic acid. Conclusion: Essential oils of selected herbs and spices retained compatibility with gingival fibroblasts in culture and showed inhibitory activity on IL-6 released by LPS-induced HGFs. These findings suggest therapeutic potential for application of assay in the management of periodontal disease.

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GC–MS Analysis of the Essential Oils of Piper nigrum L. and Piper longum L.

Cited by 51 publications

References 24 publications

Anthelmintic Activity of Fruit Extract and Fractions of Piper longum L. In vitro

Anthelmintic Activity of Fruit Extract and Fractions of Piper longum L. In vitro

Rapid determination of volatile composition from Polygala furcata Royle by MAE–HS-SPME followed by GC–MS

Anti-Inflammatory Activity of Selected Edible Herbs and Spices on Cultured Human Gingival Fibroblasts

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