GC-MS Analysis of Potentially Volatile Compounds of Pleurotus ostreatus Polar Extract: In vitro Antimicrobial, Cytotoxic, Immunomodulatory, and Antioxidant Activities

Abstract

One strategy to manage resistant pathogens and develop potential anticancer drugs is the search for new, promising, and cost-effective medicinal benefits in the field of bioactive metabolites derived from mushrooms. In the current study, Egyptian cultivated Pleurotus ostreatus fruiting bodies polar extract was prepared to evaluate its antimicrobial activities as well as its cytotoxic effect on various cancer cell lines. The Pleurotus ostreatus polar extract (PoPE) was characterized by its phenolic and flavonoid content. The phenolics and flavonoids of PoPE were 6.94 and 0.15 mg/g, respectively. P. ostreatus polar extract showed potent antimicrobial activity against four pathogens, including Candida albicans, Staphylococcus aureus, Micrococcus luteus, and Escherichia coli. PoPE was found to inhibit Fusarium oxysporum (47%), Fusarium solani (28%) as well as Rhizoctonia solani (21%). PoPE was found to be 13 times more selective and toxic to MCF-7 cells than Vero normal cells, with the lowest IC50 value (4.5 μg/mL), so they were selected to examine the potential cytotoxic effects of PoPE. In MCF-7 cells, PoPE appeared to promote cell cycle arrest in the sub-G1 stage, as well as apoptosis. It significantly increased TNF-α production while decreasing IL-6 levels. PoPE’s total antioxidant capacity, lipid peroxide, and glutathione reductase activity were recorded 0.14 ± 0.02 mM/L, 15.60 ± 0.015 nmol/mL, and 9.50 ± 1.30 U/L, respectively. The existence of different bioactive metabolites was investigated via GC-MS, which confirmed the presence of 15 compounds with well-known biological activity.

Metabolomic Profiling, Antibacterial, and Molluscicidal Properties of the Medicinal Plants Calotropis procera and Atriplex halimus: In Silico Molecular Docking Study

“…Each bacterium was then inoculated with a single colony and cultivated for 24 h at 37 °C in a nutrient broth medium. The antibacterial activities of plants’ extracts were studied using the well diffusion technique, as explained by [ 59 ]. Plates (9 mm) containing 20 mL of nutritional agar medium were inoculated with 100 µL of the bacterial suspension (1 × 106 CFU/mL).…”

Oyster mushroom with short cropping duration and good taste developed through mono-monokaryon hybridization

“…Volatile secondary metabolites play a vital pharmaceutical role in addition to contributing aroma and avor of the mushroom. Various types of these volatile metabolites found in the present study are reported to function as anticancer, anti-in ammatory, antimicrobial, antioxidant, and other health-related activities (Aparna et al 2012;Yu et al 2013;Kim et al 2020;Hamad et al 2022). Eight volatile compounds are present commonly in all the stains of P. djamor and some other speci c metabolites are found in each strain of P. djamor.…”

Acetylcholine Esterase Inhibitory Effect, Antimicrobial, Antioxidant, Metabolomic Profiling, and an In Silico Study of Non-Polar Extract of The Halotolerant Marine Fungus Penicillium chrysogenum MZ945518

“…According to Hamad et al [ 19 ], the antibacterial and anticandidal activities of the crude extract were evaluated as follows: 100 μL of the previously cultured bacterial and Candida albicans suspensions, each containing 1 × 10 8 CFU/mL (OD 600 ~0.1) were distributed onto the surfaces of nutrient agar and PDA media, respectively. A 6-mm sterile cork borer was used to produce wells in the agar plates.…”