GC-MS Analysis of Bioactive Phytochemicals Present in Ethanolic Extracts of Leaves of Annona muricata: A Further Evidence for Its Medicinal Diversity

Abstract

Background: Folk medicine has taken an important place especially in developing countries where limited health services are available. However, the absence of scientific evaluation of medicinal plants may cause serious adverse effects. Objective: To analyze the phytochemical composition of the ethanolic extracts of leaves of Annona muricata using gas chromatography mass spectroscopy (GC-MS). Materials and methods: GC-MS Analysis was used. Results: The GC-MS Analysis revealed 25 constituents of which 12 of the compounds were identified. The major constituents were two unidentified compounds with percentage peak areas of 23.51% and 16.8%. Of the identified compounds, the outstanding in composition were 7-Tetradecenal, (Z) (peak area 9.39%), n-Hexadecanoic acid (peak area 7.12%), Oleyl Alcohol (peak area 6.15%), Phytol (peak area 5.61%), cis, cis, cis-7,10,13-Hexadecatrienal (peak area 4.26%), 2-Pentadecanol (peak area 3.93%), 9,12-Octadecadienoic acid, ethyl ester (peak area 3.21%), 1,2-Benzenedicarboxylic acid, butyl octyl ester (peak area 2.67%), and 1,E-11,Z-13-Octadecatriene (peak area 2.15%), while the rest had less than 2% composition by peak area. Conclusion: The current study suggests that ethanolic extracts of leaves of Annona muricata are a potent therapeutic agent and paves the way for the development of several treatment regimens based on compounds from this extract.

Comparative GC-MS Analysis of Bioactive Phytochemicals from Different Plant Parts and Callus of Leptadenia reticulata Wight and Arn.

“…It has antioxidant, hemolytic, anti-androgenic, nematicide and pesticidal activities and also acts as 5-alpha reductase inhibitor. 60 Vitamin C compound l-(+)-Ascorbic acid 2,6-dihexadecanoate was found in abundance in EACE (20.78%), MRE (18.66%), EASE (17.55%), MCE (16.18%). This shows potential antimicrobial, anti-cancerous 71 and antioxidant activity.…”

Phytocompounds of Anonna muricata leaves extract and cytotoxic effects on breast cancer cells

“…Based on the guidelines set by the Americal National Institute, the activity limit for crude extract for IC 50 after 72 h of exposure is 30 μg/mL [43]. Based on a study by Gavamukulya [15] and co-workers, GCMS analysis of ethanolic extract of leaves of A. muricata contained 25 constituents, the major constituents were 7-tetradecenal, n-hexadecanoic acid, oleryl alcohol, phytol, cis, cis, cis-7,10,13-hexadecatrienal, 2-pentadecanol, 9,12-Octadecadienoic acid, ethyl ester, 1,2benzenedicarboxylic acid, butyl octyl ester, and 1,E-11,Z-13octadecatriene [44]. On the other hands, the main constituents found in methanol extract of the leaves are 4H-pyran-4-one, 2,3-dihydro-3,5dihydroxy-6, tetradecanoic acid, 3,7,11,15-tetramethyl-2-hexadecen-1-ol, hexadecanoic acid methyl ester, n-hexadecanoic acid, phytol and octadecanoic acid [17].…”

“…To date, gas chromatography mass spectrometry (GCMS) remains an important tool to identify compounds and their percentage in a complex mixture of crude extracts. GCMS analysis that has been done on ethanol [15,16] and methanol [17] leaves extract of A. muricata [18], identified a few types of acetogenins [muricatalicin (I), muricatalin (VI) and annonacin-B mesitoate (Vb)], which have potential antineoplastic activities. Our study was carried out to identify the compounds present in the A. muricata n-hexane-, ethyl acetate-, methanol-, and aqueous leaves extract by GCMS.…”

The effect of ethanolic leaves extract of soursop (Annona muricata L.) on human colorectal cancer cell line: cell viability and in silico study to cyclin D1 protein

“…Docking simulation of extract Annona muricata L. to cyclin D1 was started by ligand (compounds) and receptor (protein) preparation. The compounds were obtained from Gavamukulya Y, et al, 17 i.e 2-pentadecanol; oleyl alcohol; 1,2-benzenedicarboxylic acid, butyl octyl ester; 3,7,11,15-tetramethyl-2hexadecen-1-ol; N-hexadecanoic acid; hexadecanoic acid, ethyl ester; phytol; 1,E-11,Z-13-octadecatriene; 7-tetradecenal, (Z); 9,12-octadecadienoic acid, ethyl ester; cis, cis, cis-7,10,13-hexadecatrienal; and 1,2-benzenedicarboxylic acid, diisooctyl ester by using GC-MS Analysis. These specific compounds have been analyzed from ethanolic leaves extract of soursop.…”