Comparative GC-MS Analysis of Bioactive Phytochemicals from Different Plant Parts and Callus of Leptadenia reticulata Wight and Arn.

Godara, Priyanka; Dulara, Bunty Kumar; Barwer, Neelam; Chaudhary, Navneet Singh

doi:10.5530/pj.2019.1.22

Cited by 33 publications

(23 citation statements)

References 46 publications

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“…Terpenes and terpenoids has important physiological as well as ecological roles and shows many biological activities such as hepato-protective, antioxidant and cholinesterase inhibitors. 11 Total ten different terpenes and terpenoids compounds were present in the extracts of D. glaucum. Neophytadiene (1.67%), a terpenoid compound, was found in the aerial part extract and has anti-inflammatory, antipyretic, antioxidant and antimicrobial properties.…”

Section: Terpenes and Terpenoidsmentioning

confidence: 99%

“…This compound known to possess anticancer, anti-inflammatory, antidiuretic, antimicrobial, 29 antioxidant, immunostimulant and cholesterol lowering properties. 11 Correspondingly, Ogunlesi et al 39 and Satyal et al 40 Anti-cancerous 47 , anti-inflammatory 48 , antioxidant, anti-androgenic, hypo-cholesterolemic, hemolytic, 5-alpha reductase inhibitor, nematicides and pesticides 19 10.…”

Section: Terpenes and Terpenoidsmentioning

confidence: 99%

“…Hexadecanoic acid, methyl ester Fatty acid ester Ae/Ca Antitumor, immunostimulant 11 , antioxidant, 5-alpha reductase inhibitor, hypo-cholesterolemic and pesticides 19…”

Section: Terpenes and Terpenoidsmentioning

confidence: 99%

“…GC-MS technique has become a key of technological platform for profiling of secondary metabolite 10 and direct analysis of unknown components existing in medicinal plants. 11 A monotypic genus with one species, Dipterygium glaucum Decne., locally known as "Phel" belongs to the family Capparidaceae is distributed across the N.E. Africa, Arabia, Sind and India.…”

Section: Introductionmentioning

confidence: 99%

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GC-MS Analysis of Bioactive Phytochemicals in Methanol Extract of Aerial Part and Callus of Dipterygium glaucum Decne

Choudhary¹,

Jk²,

Kataria³

2019

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Introduction: This study was designed to evaluate the phytocomponents present in aerial part and in vitro induced callus methanol extracts of Dipterygium glaucum by Gas Chromatography-Mass Spectrometry (GC-MS) technique. Methods: GC-MS analysis of aerial part sample and callus produced from leaf of D. glaucum extracted in methanol solvents was performed using GC-MS QP 2010 Plus (Shimadzu, Japan) system comprising an auto sampler (AOC-20i) and a gas chromatograph interfaced to a mass spectrometer. Results: This study was carried out to identified and comparative analysis of bioactive phytochemicals from aerial part extract and callus extract of D. glaucum. This analysis revealed that both the extracts have 69 different types of phytochemical components in varying quantities. Some of the important phytochemical compounds were Stigmasterol, β-sitosterol, γ-sitosterol, Campesterol, Squalene, n-Hexadecanoic acid, Stearic acid, Myristic acid, Quinazoline, Linalyl acetate etc. These chemical compounds have anticancer, antitumor, anti-inflammatory, antidiabetic and antioxidants properties. Conclusion: This study represents the detection and identification of different phytochemical compounds from aerial part and callus extract of D. glaucum. Thus, due to the presence of various important bioactive phytocomponents this plant is recommended as a pharmaceutically important plant.

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Section: Terpenes and Terpenoidsmentioning

confidence: 99%

Section: Terpenes and Terpenoidsmentioning

confidence: 99%

“…Hexadecanoic acid, methyl ester Fatty acid ester Ae/Ca Antitumor, immunostimulant 11 , antioxidant, 5-alpha reductase inhibitor, hypo-cholesterolemic and pesticides 19…”

Section: Terpenes and Terpenoidsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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GC-MS Analysis of Bioactive Phytochemicals in Methanol Extract of Aerial Part and Callus of Dipterygium glaucum Decne

Choudhary¹,

Jk²,

Kataria³

2019

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“…In addition, vitamin E has been reported to have anti-cardiovascular, anti-dementia, anticonvulsant, antioxidant, neuroprotector activities, and improving neurological dysfunction [98,99]. Campesterol has anticancer, antioxidant, hypocholesterolemic, anxiolytic, and antidepressant properties [100,101] whereas stigmasterol has anti-inflammatory and neuroprotective activities, and also responsible for reducing oxidative stress [95,102]. Elaidic acid is responsible for reducing oxidative stress and neurotoxicity [103].…”

Section: Compoundsmentioning

confidence: 99%

Intervention in Neuropsychiatric Disorders by Suppressing Inflammatory and Oxidative Stress Signal and Exploration of In Silico Studies for Potential Lead Compounds from Holigarna caustica (Dennst.) Oken leaves

et al. 2020

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Holigarna caustica (Dennst.), a popular plant used in folk medicine in Bangladesh, is often used by the local folk practitioner to treat a variety of chronic diseases. The present research is an attempt to find out an innovative therapeutic prospect for the management of neuropsychiatric disorders. The methanol extract of H. caustica leaves (MEHC) were utilized on various behavioral tests for assessing anxiolytic, anti-depressant, and anti-inflammatory activities. The antioxidant potentials and quantitative phytochemicals were evaluated through spectrophotometric methods. Results revealed that treatment of MEHC (200 and 400 mg/kg) significantly reduced anxiety like behaviors in mice, particularly, 400 mg/kg efficiently improved % of entries and time spent (p < 0.05) in the open arms in elevated plus maze test, whereas, superior head dipping tendency (p < 0.05) was observed in hole-board test. In contrast, mice treated with 200 mg/kg revealed better anxiolytic effect in both open field and hole-cross tests. During antidepressant evaluation, mice administrated with MEHC exhibited active behaviors (swimming and struggling) in forced swimming and tail suspension tests. In parallel, MEHC manifested a noteworthy (p < 0.001) suppression of inflammatory response induced by histamine. The MEHC also showed strong antioxidant activities in 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) (IC50: 57.64 μg/mL) scavenging, H2O2 (IC50: 51.60 μg/mL) scavenging, and ferric reducing power assay. The levels of total phenol, flavonoid, flavonol, condensed tannin, and antioxidant were estimated as higher in MEHC. Moreover, 11 compounds were documented as bioactive, displayed good binding affinities to potassium channel receptor, human serotonin receptor, cyclooxygenase (COX-1 and 2), and xanthine oxidoreductase enzyme targets in molecular docking experiments. Furthermore, ADME/T and Prediction of Activity Spectra for Substances (PASS) analyses exposed their drug-likeness, nontoxic upon consumption, and likely pharmacological actions. Overall, the H. caustica is potentially bioactive as evident by in vivo, in vitro, and computational analysis. Our findings support the folkloric value of this plant, which may provide a potential source towards developing drug leads.

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Development and validation of high‐performance thin‐layer chromatography method for simultaneous estimation of α‐Amyrin, Betulin, and β‐Sitosterol in Leptadenia reticulata and Marsdenia tenacissima

Robert,

Aeri

2023

Separation Science Plus

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High‐performance thin‐layer chromatography (HPTLC), a top‐notch technique in the field of analysis, is quite irreplaceable when it comes to identifying and standardizing medicinal plants. Marsdenia tenacissima and Leptadenia reticulata have claimed traditional uses like anti‐cancer, anti‐inflammatory, anti‐depressant, hepatoprotection, etc., with few similar phytoconstituents. In this study, a precise and validated HPTLC densitometric method was developed for the identification of α‐amyrin, β‐sitosterol, and betulin in the above‐mentioned plants by static maceration with ethanol and aqueous‐ethanol extracts. The developed method was evaluated by linearity, range, accuracy, precision, limit of detection, limit of quantification, robustness, and specificity. The markers were subjected to ascending development with toluene: ethyl acetate: glacial acetic acid at a ratio of 8:1.5:0.5 v/v/v, air‐dried, and derivatized with anisaldehyde‐sulfuric acid. The retardation factor values of the markers were found to be 0.63 (α‐amyrin), 0.52 (β‐sitosterol), and 0.64 (betulin) for L. reticulata, and 0.55 (α‐amyrin), 0.45 (β‐sitosterol), and 0.57 (betulin) for M. tenacissima. The chromatographic results suggest that the developed method suits all three markers and can be simultaneously identified in both plants.

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Comparative GC-MS Analysis of Bioactive Phytochemicals from Different Plant Parts and Callus of Leptadenia reticulata Wight and Arn.

Cited by 33 publications

References 46 publications

GC-MS Analysis of Bioactive Phytochemicals in Methanol Extract of Aerial Part and Callus of Dipterygium glaucum Decne

GC-MS Analysis of Bioactive Phytochemicals in Methanol Extract of Aerial Part and Callus of Dipterygium glaucum Decne

Intervention in Neuropsychiatric Disorders by Suppressing Inflammatory and Oxidative Stress Signal and Exploration of In Silico Studies for Potential Lead Compounds from Holigarna caustica (Dennst.) Oken leaves

Development and validation of high‐performance thin‐layer chromatography method for simultaneous estimation of α‐Amyrin, Betulin, and β‐Sitosterol in Leptadenia reticulata and Marsdenia tenacissima

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