GATA4 is a transcriptional regulator of R-spondin1 in Japanese flounder (Paralichthys olivaceus)

Liu, Xiumei; Li, Zan; Wang, Bo; Zhu, He; Liu, Yuezhong; Qi, Jie; Zhang, Quanqi

doi:10.1016/j.gene.2018.01.041

Cited by 5 publications

(1 citation statement)

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“…Previous study has shown that endogenous promoters could drive higher levels of expression [ 33 ]. Although the Japanese flounder β-actin gene has been reported as a reference gene in the literature [ 34 , 35 ], little was known about the functional characterization of its promoter region. In this study, a series of experimental tests were performed to gain more insight into the application of the Poβ-actin promoter and its compact construct in foreign gene expression research.…”

Section: Discussionmentioning

confidence: 99%

Functional Analysis of the Promoter Region of Japanese Flounder (Paralichthys olivaceus) β-actin Gene: A Useful Tool for Gene Research in Marine Fish

Wang

Gao

et al. 2018

IJMS

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A newly isolated Japanese flounder (Paralichthys olivaceus) β-actin promoter and its derivative compact construct Poβ-actinΔ−1080/−801Δ−500/−201 have recently been demonstrated to promote ectopic gene expression in cell lines. Different Poβ-actin promoter deletion mutants were constructed and functionally characterized. Mutational analyses by dual-luciferase detected that three regulatory elements, including one enhancer (−1399/−1081) and two silencers (−1080/−801, −500/−201) in the first intron. The sequence located at −1399/−1081 was determined to significantly affect promoter activity. Additionally, the first exon (−1489/−1400) could also remarkably promote the β-actin promoter activity. In the following transduction application, we removed the two silencers and generated a compact reconstruct promoter/enhancer (Poβ-actinΔ−1080/−801Δ−500/−201), which exhibited relatively stronger promoter activity compared with Poβ-actin. Furthermore, the green fluorescent protein (GFP) transgenic stable flounder cell line was obtained by the reconstructed Poβ-actinΔ−1080/−801Δ−500/−201 promoter. Our study provided the potential application of Japanese flounder β-actin, particularly Poβ-actinΔ−1080/−801Δ−500/−201, in ectopic gene expression in the future.

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Section: Discussionmentioning

confidence: 99%