GATA3 expression in morphologic subtypes of breast carcinoma: a comparison with gross cystic disease fluid protein 15 and mammaglobin

Wendroth, Scott M.; Mentrikoski, Mark J.; Wick, Mark R.

doi:10.1016/j.anndiagpath.2014.12.001

Cited by 67 publications

(42 citation statements)

References 28 publications

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“…Their cohort included a higher number of ER negative carcinomas than our study and Shield et al Another study performed on cell blocks showed sensitivity for GATA-3, MAM and GCDFP-15 to be 93.5 %, 22.4 % and 5.2 % respectively [18]. Surprisingly, GCDFP-15 performed much better in tissue specimens of primary and metastatic carcinomas staining 54.4 % of ductal carcinomas, 48.3 % of lobular carcinomas, and 81 % of apocrine mammary carcinomas [19]. When studied in ER negative carcinomas, Liu et al reported a sensitivity of 15 % [20] compared to our 50 % in ER negative carcinomas.…”

Section: Discussioncontrasting

confidence: 47%

Comparison of GATA-3, mammaglobin, GCDFP-15 expression in breast carcinoma in serous effusions: A cell-block micro-array study

Hag

et al. 2017

Pleura and Peritoneum

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Background: GATA-3 is a potential marker for detection of metastatic breast carcinoma, reportedly more sensitive than mammaglobin (MAM) and GCDFP-15. We aim to compare the sensitivity of GATA-3, MAM and GCDFP-15 in determining the breast origin of malignant effusions. Methods: Cell blocks from 27 cases of serous effusions positive for metastatic breast cancer were retrieved. Immunohistochemistry for GATA-3, MAM, gross cystic disease fluid protein 15 (GCDFP-15), estrogen receptor (ER) and progesterone receptor (PR) was performed on cell-block micro-array. Statistical analysis using two ways Chi square, one-way ANOVA and multiple regression was performed. Results: The detection rate of breast cancer in serous fluid was significantly higher with GATA-3 (88.8 %, X2 = 15.9, p = 0.00034) than with MAM (51.8 %) and GCDFP-15 (37.0 %). All ER positive cases (19) were GATA-3 positive. Conversely, all GATA-3 negative cases (3) were ER negative. The intensity of stain and percentage of positive cells were significantly higher with GATA-3 (p < 0.0001) than with MAM and GCDFP-15. The intensity and percentage of positive cells score of GATA-3 were statistically associated with ER stain intensity and percentage of positive cell scores.

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Section: Discussioncontrasting

confidence: 47%

Comparison of GATA-3, mammaglobin, GCDFP-15 expression in breast carcinoma in serous effusions: A cell-block micro-array study

Hag

et al. 2017

Pleura and Peritoneum

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“…It was not therefore surprising that none of them expressed GATA3. However, some researchers have reported the opposite result, demonstrating GATA3 staining in apocrine carcinomas 18 45. The expression pattern of GATA3 in apocrine breast cancers therefore remains to be determined.…”

Section: Discussionmentioning

confidence: 98%

“…This percentage is lower than that reported by Miettinen et al 15 who considered any nuclear labelling for GATA3 as a positive result, while we used a 5% threshold for positive immunostaining. Wendroth et al 18 also used 5% as the threshold for a positive staining result for GATA3 and they obtained an even higher percentage (91.5%) for immunostaining tumours. The reason for this discrepancy is probably that they included a much smaller number of cases (153 cases in total) in their study.…”

Section: Discussionmentioning

confidence: 99%

“…We adopted 5% as the cut-off point between positive and negative staining for the three markers, as used by some investigators in the literature 18 19 30–32…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, GATA3 has been shown to be more sensitive in breast carcinomas at both the protein and mRNA level than gross cystic disease fluid protein 15 (GCDFP15) and mammaglobin, which have long been used most commonly as confirmatory immunomarkers of mammary origin in the context of metastasising lesions. The immunostaining of GATA3 is nuclear and is stronger and more diffuse than the other two markers, the staining of which is cytoplasmic 17 18. Therefore, GATA3 is helpful in cases with small tissue samples or problematic background staining.…”

Section: Introductionmentioning

confidence: 99%

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Characterisation of GATA3 expression in invasive breast cancer: differences in histological subtypes and immunohistochemically defined molecular subtypes

Tang

et al. 2017

J Clin Pathol

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A pilot study: Comparison of TRPS1 and GATA3 immunoperoxidase staining using cytologic smears in entities reportedly positive for GATA3

Rohra

Ding

Yoon

et al. 2022

Cancer Cytopathology

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BACKGROUND: Metastatic breast carcinoma (mBC) is frequently encountered and may be challenging to diagnose as the tumor cells can morphologically resemble carcinomas of other primary origins. An additional challenge is that direct smears are often the only sample type available for immunostaining studies in cytology. Trichorhinophalangeal syndrome GATAbinding type 1 1 (TRPS1) is a highly sensitive marker for BC compared to the commonly used marker GATA3, especially in triple-negative BC (TNBC), in histologic samples. However, its sensitivity and specificity in mBC and other GATA3-positive tumors have not been studied. METHODS: The authors identified the following cytology cases: 37 GATA3-positive mBC cases and 19 available cases that were deemed mBC but were GATA3-negative during the original case workup and five cases of each of eight epithelioid entities known to have high rates of GATA3 positivity and commonly seen in cytology practice. Immunostainings of TRPS1 and GATA3 were performed on the chosen smears following standard protocols. RESULTS: TRPS1 was positive in all 37 GATA3-positive mBC cases and in 18 of the 19 GATA3-negative mBC cases. TRPS1 was negative in all five of the seven frequently GATA3-positive epithelioid entities, with the exception of salivary duct carcinomas where GATA3 was positive in a rate ranging 60%-100% among them. CONCLUSIONS: TRPS1 is as sensitive as GATA3 in GATA3positive mBC and is more sensitive than GATA3 in TNBC. TRPS1 is negative in most GATA3-positive nonbreast tumors. Thus, the combination of TRPS1 and GATA3 could be used to differentiate breast primary from others in most situations.

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GATA3 expression in morphologic subtypes of breast carcinoma: a comparison with gross cystic disease fluid protein 15 and mammaglobin

Cited by 67 publications

References 28 publications

Comparison of GATA-3, mammaglobin, GCDFP-15 expression in breast carcinoma in serous effusions: A cell-block micro-array study

Comparison of GATA-3, mammaglobin, GCDFP-15 expression in breast carcinoma in serous effusions: A cell-block micro-array study

Characterisation of GATA3 expression in invasive breast cancer: differences in histological subtypes and immunohistochemically defined molecular subtypes

A pilot study: Comparison of TRPS1 and GATA3 immunoperoxidase staining using cytologic smears in entities reportedly positive for GATA3

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