Gastroprotective activity of the ethanolic extract and hexane phase of Combretum duarteanum Cambess. (Combretaceae)

Abstract

Combretum duarteanum Cambess. is found in South America, particularly in Bolivia, Paraguay, and Brazil. In Paraiba state (Brazil), the species usually occurs in the Caatinga biome. It is popularly known as mofumbo, cipiúba, or cipaúba. This work aims to evaluate the gastroprotective activity and the cytoprotective mechanisms of the ethanolic extract (Cd-EtOHE) and hexane phase (Cd-HexP) obtained from the leaves of C. duarteanum. Doses at 62.5, 125, 250, and 500 mg/kg of Cd-EtOHE and Cd-HexP were tested in models of gastric ulcers induced by HCl/ethanol, absolute ethanol, stress, non-steroidal anti-inflammatory drugs, and pylorus ligation in male rats or mice. Cd-EtOHE and Cd-HexP significantly reduced gastric injuries induced in all models. Cd-EtOHE and Cd-HexP did not alter gastric juice parameters such as pH, [H(+)], or volume after pylorus ligation. Cytoprotective mechanisms of Cd-EtOHE and Cd-HexP in relation to mucus, nitric oxide (NO), and sulfhydryl (SH) groups were evaluated. Neither product increased the mucus, and they both showed dependence on NO and SH groups to prevent gastric ulcer. Both Cd-EtOHE and Cd-HexP demonstrated gastroprotective activity.

Atividade antimicrobiana da jurema preta (Mimosa tenuiflora (Wild) Poir.)

“…O resultado demonstrou ação antimicrobiana frente à cepa estudada (BEZERRA et al, 2009;CAVALCANTE, 2019;PADILHA et al, 2010). Lima et al (2011) avaliando a ação antibacteriana de plantas taníferas em bactérias anaeróbicas isoladas da pododermatite ovina, mostrou que os extratos hidroalcoólicos da Jurema preta (M. tenuiflora), apresentaram atividade antimicrobiana in vitro sobre os microrganismos Dichelobacter nodosus, Fusobacterium necrophorum, Bacteroides fragilis, Prevotella melaninogenica, Bacteroides intermedius, Bacteroides ovatus e Fusobacterium perfoetens. avaliando a atividade antifúngica da Jurema Preta em comparação com antifúgicos convencional em casos de mastite subclínica no interior de Pernambuco, verificou que o extrato da casca da Jurema Preta apresentou atividade antifúngica bastante satisfatória sobre a levedura de Candida albicans, proporcionando resultado superior aos obtidos com o antifúngico sintético.…”

Anti-inflammatory intestinal activity ofCombretum duarteanumCambess. in trinitrobenzene sulfonic acid colitis model

“…This shrubby species is exclusive to South America with registrations in Bolivia, Paraguay, and Brazil. It occurs in the northern and northeastern regions of Brazil, being associated with the “caatinga” biome[ 15 , 16 ].…”

“…In folk medicine, C. duarteanum is used to treat pain, inflammation and GI tract disorders, which justifies its selection, using ethno-pharmacological indication as the criterion of choice. Phytochemical studies suggest the presence of flavonoids and triterpenes, whose pharmacological effects have been attributed[ 16 - 18 ].…”

“…de Morais Lima et al[ 16 , 19 ] demonstrated gastro-protective and antiulcer activity in C. duarteanum in different models of acute ulcer induction (acidified ethanol, ethanol, nonsteroidal anti-inflammatory drugs (commonly known as NSAIDs), stress, pylorus ligature, acetic acid) in animals. Previous studies demonstrated low toxicity and no change of liver enzymes in animals treated with the tested plant sample for 15 d in acid acetic-induced gastric ulcer model[ 19 ].…”