Gastroprotective action of the ethanol extract of Leonurus sibiricus L. (Lamiaceae) in mice

Biano, Laiza S.; Oliveira, Alan Santos; Palmeira, David N.; Silva, Luiz André Santos; Albuquerque‐Júnior, Ricardo Luiz Cavalcanti de; Duarte, Marcelo Cavalcante; Corrêa, Cristiane Bani; Grespan, Renata; Batista, Josemar Sena; Camargo, Enilton A.

doi:10.1016/j.jep.2021.114792

Cited by 2 publications

(4 citation statements)

References 45 publications

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“…To determine the gastric juice’s parameters, a mouse model of pyloric ligation-induced gastric ulcers was used [ 24 ]. Similarly, the mice were divided into four groups (NM, MD, HFPH, and LFPH) ( n = 10).…”

Section: Methodsmentioning

confidence: 99%

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Protective Effect of Foxtail Millet Protein Hydrolysate on Ethanol and Pyloric Ligation-Induced Gastric Ulcers in Mice

et al. 2022

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Foxtail millet has been traditionally considered to possess gastroprotective effects, but studies evaluating its use as a treatment for gastric ulcers are lacking. Here, we assessed the antiulcer effects of foxtail millet protein hydrolysate (FPH) and explored its mechanism by using blocking agents. In a mouse model of ethanol-induced gastric ulcers, pretreatment with FPH reduced the ulcerative lesion index, downregulated the expression of inflammatory cytokines in the gastric tissue, increased the activity of antioxidant enzymes, and improved the oxidative status. FPH increased constitutive the activity of nitric oxide synthase (cNOS), NO levels, and mucin expression in gastric mucosa, and inhibited the activation of the ET-1/PI3K/Akt pathway. In a mouse model of pyloric ligation-induced gastric ulcers, FPH inhibited gastric acid secretion and decreased the activity of gastric protease. Pretreatment of mice with the sulfhydryl blocker NEM and the NO synthesis inhibitor L-NAME abolished the gastroprotective effect of FPH, but not the KATP channel blocker glibenclamide and the PGE2 synthesis blocker indomethacin. Among the peptides identified in FPH, 10 peptides were predicted to have regulatory effects on the gastric mucosa, and the key sequences were GP and PG. The results confirmed the gastroprotective effect of FPH and revealed that its mechanism was through the regulation of gastric mucosal mucus and NO synthesis. This study supports the health effects of a millet-enriched diet and provides a basis for millet protein as a functional food to improve gastric ulcers and its related oxidative stress.

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Section: Methodsmentioning

confidence: 99%

“…After 14 days of FPH gavage, the mice were fasted for 24 h and anesthetized by an intraperitoneal injection of a pentobarbital sodium solution (15 mg/mL, 2 mL). An abdominal incision was made and the pylorus was ligated according to previously reported methods [ 24 ]. Except for the NC group, all the other groups underwent pyloric ligation.…”

Section: Methodsmentioning

confidence: 99%

Protective Effect of Foxtail Millet Protein Hydrolysate on Ethanol and Pyloric Ligation-Induced Gastric Ulcers in Mice

et al. 2022

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“…In the east of the State of Paraná in Brazil, people use L. japonicus as folk medicine, macerating the fresh leaves and adding them over the wounds as a “cataplasma” [11] . The extracts of leaves and flowers are utilized for stomach disorders such as vomiting, dysentery, and trapped gas [12] . The leaf extracts treat rheumatism and dermatitis, [13] and the dried plant for menstrual pain, dysmenorrhea, amenorrhea, and renal disease [14,15] .…”

Section: Introductionmentioning

confidence: 99%

“…[11] The extracts of leaves and flowers are utilized for stomach disorders such as vomiting, dysentery, and trapped gas. [12] The leaf extracts treat rheumatism and dermatitis, [13] and the dried plant for menstrual pain, dysmenorrhea, amenorrhea, and renal disease. [14,15] Other works relate cardiovascular protection, neuroprotection, and anticancer activity.…”

Section: Introductionmentioning

confidence: 99%

Preliminary Evaluation of Wound Healing Potential of Leonurus japonicus Houtt. Extracts

de Assunção Morais,

Koga,

Klein

et al. 2023

Chemistry & Biodiversity

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Leonurus japonicus Houtt. is a medicinal plant popular in Brazil as “rubim”, used in local folk medicine for several applications as an anti‐inflammatory, antioxidant, analgesic, and antimicrobial phytomedicine. The traditional use for wound healing is related; however, few studies have evaluated the wound healing activity. Thus, this study aimed to analyse the popular indication of the hydroalcoholic and aqueous extracts of L. japonicus aerial parts in a rat wound healing model. The initial chemical characterization was performed using flavonoid quantification and complemented with mass spectroscopy/chemometrics analysis. The wound‘s lesion contraction and tissue regeneration (histological study stained with hematoxylin‐eosin and picrosirius) were determined. Hydroalcoholic and aqueous extracts presented high flavonoid content, and mass spectrometry analysis of the extracts demonstrated the presence of compounds with a mass between 100–650, reinforcing the presence of polyphenolic constituents. The extracts of L. japonicus improve various wound healing phases, like inflammatory modulation, wound contraction, and collagen synthesis, resulting in faster healing in rats. These effects could be related to the extracts′ polyphenolic compounds.

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Gastroprotective action of the ethanol extract of Leonurus sibiricus L. (Lamiaceae) in mice

Cited by 2 publications

References 45 publications

Protective Effect of Foxtail Millet Protein Hydrolysate on Ethanol and Pyloric Ligation-Induced Gastric Ulcers in Mice

Protective Effect of Foxtail Millet Protein Hydrolysate on Ethanol and Pyloric Ligation-Induced Gastric Ulcers in Mice

Preliminary Evaluation of Wound Healing Potential of Leonurus japonicus Houtt. Extracts

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