Gastroileal nitrogen and electrolyte movements after bovine milk ingestion in humans

Mahé, Sylvain; Huneau, Jean‐François; Marteau, Philippe; Thuillier, F.; Tomé, Daniel

doi:10.1093/ajcn/56.2.410

Cited by 86 publications

(36 citation statements)

References 32 publications

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“…Calbet and Holst [33] would suggest, however, that when all variables such as energy density, pH, osmolality, volume, and nitrogen content are matched, relatively similar rates of appearance of amino acids in blood for plant and animal intact proteins and hydrolysates are observed. Directly applicable to our findings is the observation that skim milk empties from the intestine more slowly (25 min) than whey, casein, and their hydrolysates (18-21 min) [44]. Although the majority of work in this area has addressed satiety hormone secretion, the specific properties of skim milk in contrast to whey and casein that govern its effects on gastric emptying, rate of appearance of amino acids in the blood, and delivery of nutrients along the intestinal tract may also provide some insight into the differences we observed in fatty acid transporter expression among skim milk, casein, and whey.…”

Section: Discussionsupporting

confidence: 64%

Dairy protein and leucine alter GLP-1 release and mRNA of genes involved in intestinal lipid metabolism in vitro

Chen¹,

Reimer²

2009

Nutrition

142

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Objective-A growing body of evidence supports an antiobesity effect of dairy products; however, the mechanisms remain unclear. The objective of this study was to explore possible intestinal mechanisms by which dairy delivers an antiobesity effect. The human intestinal cell line, NCI-H716, was used to test the hypothesis that branched-chain amino acids and dairy proteins regulate satiety hormone secretion and modulate genes involved in fatty acid and cholesterol metabolism.Methods-In dose-response (0.5%, 1.0%, 2.0%, and 3.0%) studies, the effect of leucine, isoleucine, valine, skim milk, casein, and whey on glucagon-like peptide-1 release and the expression of selected genes were tested.Results-Leucine, isoleucine, skim milk, and casein stimulated glucagon-like peptide-1 release (P < 0.05). Isoleucine and whey downregulated the expression of intestinal-type fatty acid binding protein (i-FABP), fatty acid transport protein 4 (FATP4), Niemann-Pick C-1-like-1 protein (NPC1L1), acetyl-coenzyme A carboxylase (ACC), fatty acid synthase (FAS), sterol regulatory element-binding protein-2 (SREBP-2), and 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR; P < 0.05). Leucine and valine downregulated the expression of NPC1L1, ACC, FAS, SREBP-2, and HMGCR (P < 0.05). Casein downregulated the expression of i-FABP, FATP4, ACC, FAS, SREBP-2, and HMGCR (P < 0.05). Skim milk downregulated the expression of ACC, FAS, and SREBP-2, but not i-FABP, FATP4, and NPC1L1.Conclusion-This work suggests that the antiobesity effect of dairy may be mediated, at least in part, by integration of events that promote glucagon-like peptide-1 secretion and inhibit expression of genes involved in intestinal fatty acid and cholesterol absorption and synthesis.

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Section: Discussionsupporting

confidence: 64%

Dairy protein and leucine alter GLP-1 release and mRNA of genes involved in intestinal lipid metabolism in vitro

Chen¹,

Reimer²

2009

Nutrition

142

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“…First, to identify the different metabolites, a nonphysiological high concentration (10 mM) was used. Then the absorption/metabolization studies were performed at a concentration of 100 M, which is the highest physiological concentration assayed by other authors (Mahé et al, 1992 …”

Section: Resultsmentioning

confidence: 99%

Absorption and Metabolization of Cytoprotective Epicatechin Thio Conjugates in Rats

Selga

Vinardell²,

Martín‐Venegas³

et al. 2010

Drug Metab Dispos

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ABSTRACT:The epicatechin (EC) thio derivatives 4␤-(S-cysteinyl)-epicatechin (Cys-EC) and 4␤-(S-cysteaminyl)-epicatechin (Cya-EC) are compounds that may provide protection from oxidation via mechanisms involving either the flavonoid moiety or the nonphenolic cysteine or cysteamine part of the molecule. Because the metabolically modified molecules may be the actual active species, we estimated the absorption/metabolization of the thio derivatives through the small intestine in vitro and studied the body distribution of the compounds and their metabolites in rats. The analysis of the samples generated was done using a highperformance liquid chromatograph coupled to a UV detector and a tandem mass spectrometer. We show that Cya-EC follows the same phase II metabolization pattern as EC, whereas Cys-EC is transported with the intact catechol moiety through the small intestine and effectively metabolized systemically. We also found that Cya-EC generates Cys-EC in vivo, which provides evidence for a Cya-EC-mediated cytoprotective effect through cysteamine/cystine exchange with subsequent cysteine transport, ubiquitously throughout the organism.

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“…An intestinal tube was passed through the nose and allowed to descend to the digestive tract, as described previously (18). The intestinal tube was used to 1) perfuse phenol red, a nonabsorbable intestinal marker, into the ileum and 2) collect intestinal samples by continuous suction 20 cm distally from the perfusion site.…”

Section: Clinical Protocolmentioning

confidence: 99%

Postprandial modulation of dietary and whole-body nitrogen utilization by carbohydrates in humans

Mariotti

Mahé

Luengo

et al. 2000

The American Journal of Clinical Nutrition

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Background: Sucrose exerts a sparing effect on whole-body protein metabolism, mainly during the absorptive phase. Objective: We aimed to characterize the acute postprandial effect of addition of sucrose on deamination of dietary and endogenous nitrogen, with particular consideration being given to the effects of bioavailability. Design: Twenty-one subjects equipped with ileal tubes ingested 15 N-labeled soy protein combined with [13 C]glycine, with (n = 10) or without (n = 11) sucrose. Dietary and endogenous ileal flow of nitrogen were determined from the ileal effluents. The kinetics of dietary amino acid transfer to the blood were characterized by 13 CO 2 enrichment in breath and 15 N enrichment in plasma amino acids. Deamination of dietary and endogenous amino acid was determined from body urea, urinary nitrogen, and 15 N enrichment. Results:13 CO 2 recovery in breath and 15 N plasma amino acid enrichments were highly correlated (R 2 ≥ 0.95, P < 0.001, for both meals) and markedly delayed by sucrose (half-13 CO 2 recovery: 274 min compared with 167 min), whereas exogenous and endogenous ileal nitrogen kinetics and balances remained unchanged. Addition of sucrose halved the early (0-2 h) deamination peak of dietary nitrogen and reduced endogenous nitrogen oxidation over the first 4 h. Both were reduced by 18-24% over the 8-h period after the meal. Conclusions: Without changing the nitrogen absorptive balance, sucrose markedly affected the bioavailability profile, which is governed by gastric emptying. Endogenous and dietary nitrogen were not spared in the same way and over the same periods, showing that the metabolism of endogenous and dietary nitrogen may be affected differently by nutritional modulation, even if the effects are of a similar magnitude over the entire postprandial period.Am J Clin Nutr 2000;72:954-62.

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Gastroileal nitrogen and electrolyte movements after bovine milk ingestion in humans

Cited by 86 publications

References 32 publications

Dairy protein and leucine alter GLP-1 release and mRNA of genes involved in intestinal lipid metabolism in vitro

Dairy protein and leucine alter GLP-1 release and mRNA of genes involved in intestinal lipid metabolism in vitro

Absorption and Metabolization of Cytoprotective Epicatechin Thio Conjugates in Rats

Postprandial modulation of dietary and whole-body nitrogen utilization by carbohydrates in humans

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