Gastric motility in ghrelin receptor knockout mice

Yang, Changqing; Wang, Weigang; Yan, Jun; Fei, Jian; Wang, Zhigang; Zheng, Qi

doi:10.3892/mmr.2012.1157

Cited by 18 publications

(16 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A previous study [23] showed that the protein content and gene expression of ghrelin and GHSR in the gastric fundus of DGP model were decreased, which was not consistent with our findings. At present, no uniform conclusion has been drawn about the time for the establishment of the DGP model and, further, each experiment has different modelling methods.…”

Section: Study On the Influence Of Acupoints Selection On Ghrelin Andcontrasting

confidence: 56%

Study on the influence of acupoints selection on ghrelin and GHSR expression in gastric fundus tissue of DGP rats

Yue¹,

Li²,

Liu³

et al. 2018

biomedicalresearch

View full text Add to dashboard Cite

Objective: To explore the acupoint selection compatibility according to different departments and to study the influence on ghrelin and GHSR expression in the gastric fundus in rats with Diabetic Gastroparesis (DGP). Methods: Sixty male SPF SD rats were given adaptive feeding for one week and were randomly divided into a blank control group, a model group, the Zusanli and Zhongwan (ZZ) group, the Zusanli and Neiguan group (ZN), as well as the Zusanli and non-meridian acupoint (ZNA) group, with 12 rats in each group. Except for the blank group, the other 48 rats were used to establish a DGP model by using streptozotocin. Acupuncture therapy was performed from the end of the tenth week for an additional four weeks. Then the rats were sacrificed and the serum and gastric fundus tissues were collected. ELISA assay was used to detect the ghrelin content in the serum. The expression of ghrelin positive cells in the gastric fundus tissue was detected by immunohistochemistry. The expression of ghrelin and GHSR mRNA in the gastric fundus tissue was detected by real-time PCR. Results: Compared with the blank group, the food intake and the expression of ghrelin and GHSR mRNA in the gastric fundus tissue in the model group were significantly increased, while the small intestinal propulsion rate, the ghrelin content in the serum and the gray value of the gastric fundus were significantly decreased (P<0.05). Compared with the model group, the expression of ghrelin and GHSR mRNA in the gastric fundus tissue, the small intestinal propulsion rate, the ghrelin content in the serum and the gray value of the gastric fundus were significantly increased (P<0.05). Compared with the ZZ group, the food intake were significantly increased in the ZN and the ZNA groups (P<0.05), and the expression of ghrelin and GHSR mRNA in the gastric fundus tissue of rats were significantly increased (P<0.05) but the ghrelin content in the serum in ZN group was significantly decreased (P<0.05). Conclusions: The improvement of gastrointestinal motility in DGP by acupuncture therapy was associated with the ghrelin content in the serum and GHSR expression in the gastric fundus. The treatment effect of local acupoints compatibility was better than that of the distal acupoints compatibility, and the acupoint selection according to department was one of the main influencing factors of acupoints compatibility.

show abstract

Section: Study On the Influence Of Acupoints Selection On Ghrelin Andcontrasting

confidence: 56%

Study on the influence of acupoints selection on ghrelin and GHSR expression in gastric fundus tissue of DGP rats

Yue¹,

Li²,

Liu³

et al. 2018

biomedicalresearch

View full text Add to dashboard Cite

show abstract

“…In rat, ghrelin has shown to increase gastric acid secretion, frequency and intensity of gastric movements in a dose dependent manner (Masuda et al, 2000). In ghrelin receptor knockout mice, ghrelin could not increase gastric emptying while in wild type animal, it could increase it dose dependently (Yang et al, 2013). Ghrelin could also increase contraction or relaxation amplitude of the smooth muscle strips in the presence of electrical field stimulation in an escalating dose model (Yang et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Effects of exogenous ghrelin on experimental reflux esophagitis model in male rat

Seyed¹,

Nabavizadeh²,

Faghihi³

et al. 2015

J. Physiol. Pathophysiol.

View full text Add to dashboard Cite

Reflux esophagitis is a common gastrointestinal disorder that affects the quality of life in patients.Esophagitis is a chronic disease that leads to inflammation of the esophagus. Ghrelin is a 28-amino acid peptide that has several endocrine and metabolic effects. The aim of this study was to investigate the effect of ghrelin on the esophagitis. Eighteen wistar rats were divided into three groups of six. Midline laparatomy was performed in the control group. In the gastroesophageal reflux disease (GERD) and GERD+Ghrelin groups, esophagitis was induced by method of Omura. Rats in GERD+Ghrelin group received 4 i.p. injections of 25 ng/g body weight ghrelin. The amount of gastric acid secretion and esophageal blood flow were measured. Gastric acid in GERD+Ghrelin group increased significantly when compared to control and GERD groups. The epithelium thickness and papillae of lamina properia length in GERD group increased significantly compared to the control group. The difference in papillae of lamina properia length between GERD and GERD+Ghrelin was statistically significant. Esophageal blood flow in GERD+Ghrelin group increased significantly in comparison with GERD group. Treatment with ghrelin could reduce esophageal mucosal damage followed by chronic exposure of acid and other gastric contents.

show abstract

“…Additionally to this ghrelin may also stimulate the production of growth hormone. The expression and transport of ghrelin receptors has been documented for the afferent vagus nerve, and functional studies have confirmed that vagal pathways are integral to ghrelin-induced stimulation of gastric motility [18]. Combined stimulation of mechanosensitive gastroesophageal afferents and ghrelin injections allowed to find out that the direct effect of ghrelin was hidden in the decreasing afferent response to the stretching.…”

Section: Role Of Afferent Nerves In Gastroduodenal Adaptationmentioning

confidence: 99%

“…The experimental studies, using ghrelin and ghrelin receptor agonists, confirmed this hypothesis, suggesting a therapeutic potential for the ghrelin receptor in the treatment of gastrointestinal motility disorders. Additionally to this effect, ghrelin acts centrally to increase mid-brain dopaminergic neurons activity, amplifies dopamine signaling and protect against neurotoxin-induced dopamine cell death in the mouse substantia nigra pars compacta [18]. In addition, ghrelin inhibits the lipopolysaccharide (LPS)-induced release of proinflammatory cytokines from peripheral macrophages and T-cell [19].…”

Section: Role Of Afferent Nerves In Gastroduodenal Adaptationmentioning

confidence: 99%

Role of Vagus Nerve in Gastroduodenal Adaptation and Cytoprotection

Sulaieva¹,

Obraztsova²

2014

AJCEM

View full text Add to dashboard Cite

Abstract:Objective: In this review we focused on understanding the cause-and-effect relationships of gastroduodenal pathology aiming to clarify the role of vagus nerve. Results: The spectrum of vagus nerve biological effects in gastroduodenal area is related to its numerous targets and a wide range of its receptors. A variety of vagus nerve effects are related to the broad expression of cholinergic receptors on the target cells: smooth muscle cells, covering and glandular epithelium of stomach and duodenum, myofibroblasts and mast cells, vascular endothelium, intramural ganglion neurons, endocrine cells, platelets and blood leukocytes. In this paper, we discussed the following issues: 1) role of sensory nerve endings in the vagal reflex regulation; 2) impact of gastrin and leptin on vagal afferentation; 3) targets of vagus efferent nerves; 4) the role of acetylcholine in regulation of functional activity of oxyntic cells; 5) relationship of vagus efferents with enteroendocrine cells; 6) the role of vagus nerve in realization of compensatory and adaptive reactions in gastroduodenal area. Conclusion: Vagus nerve is one of the key regulators of mucosal activity and blood supply, modulating adaptive reactions and maintaining the gastrointestinal barrier. Keywords: Gastroduodenal Area, Vagus Nerve, Afferent and Efferent NervesGastrointestinal pathology takes one of the leading places in the morbidity rate. A long-time comprehensive study has helped in determining the spectrum of exogenous and endogenous pathological factors that are associated with gastroduodenal pathology. It can be stated, that issues of digestive system regulation has been in focus of scientist for more than two centuries [1]. Nevertheless, pathogenesis of such disorders as gastroesophageal reflux disease, gastritis, peptic ulcer disease, duodenitis is still not clear.Structural homeostasis and functions of gastroduodenal area (GDA) are controlled by neural link, specific gastrointestinal regulatory peptides that have endo-, paraand neuroendocrine effects; and local factors -cytokines, growth factors, nitric oxide, etc. [2-3]. These regulatory molecules are produced by various cells of the mucosa and neurons of intramural ganglia. The auto-regulation, synchronicity, sequence, integration and protection of digestive organs are achieved due to the potentiation and modulation of these molecules [4,5]. At the same time there is a hierarchy of GDA regulatory mechanisms, due to which any disorder automatically activates higher levels of management and control, that's why neuro-endocrine control goes on the forefront [3,6]. Initially, the centers of autonomic nervous systems are activated that leads us to the aim of this review to fully analyze the structural organization of the parasympathetic innervation and role of vagus nerve in the pathology and compensatory-adaptive processes in GDA.Vagus nerve dependent gastric reflexes are mediated through vagal afferent fibers synapsing upon neurons of the nucleus tractus solitarius which, in turn, modulates the...

show abstract

Gastric motility in ghrelin receptor knockout mice

Cited by 18 publications

References 42 publications

Study on the influence of acupoints selection on ghrelin and GHSR expression in gastric fundus tissue of DGP rats

Study on the influence of acupoints selection on ghrelin and GHSR expression in gastric fundus tissue of DGP rats

Effects of exogenous ghrelin on experimental reflux esophagitis model in male rat

Role of Vagus Nerve in Gastroduodenal Adaptation and Cytoprotection

Contact Info

Product

Resources

About