Gastric Antral Ulcer Produced by Diethyldithiocarbamate in Rats

Abstract

A subcutaneous dose of diethyldithiocarbamate (DDC) combined with a topical application of hydrochloric acid produced deep ulcers in the lesser curvature of the antrum in pylorus-ligated rats. The ulcers penetrated the tunica muscularis and formed an ulcer with an incidence of 100%. A significant decrease in Cu,Zn-superoxide dismutase (SOD) activity and a significant increase in the level of thiobarbituric acid (TBA) reactants in the antral mucosa occurred prior to the ulcer formation. Treatment with SOD and allopurinol significantly inhibited antral ulceration. These results suggest that superoxide radicals are involved in the pathogenesis of the ulcer and that this ulcer model is useful for studying gastric ulcer etiology.

Optimization of Suppository Preparation Containing Sodium Laurate and Taurine That Can Safely Improve Rectal Absorption of Rebamipide

“…AUC, C max and BA of rebamipide for suppository j were significantly greater than those for suppository b, containing 25-mg C10 (pϽ0.05), indicating that suppository j is better than commercial products in terms of absorption enhancement. As rebamipide is a poorly watersoluble and poorly absorbable drug, [14][15][16][17][18][19][20] the improvement of both dissolution and membrane permeability should be needed to enhance the absorption of rebamipide. The enhancement of membrane permeability by C12 was already The compositions of suppositories examined are summarized in Table 3.…”

“…As for the improvement of permeability, the use of absorption enhancers is well known, [1][2][3][4][5][6][7][8][9][10][11] but the practical use of them has been very limited 12,13) because of the potential local toxicity to gastrointestinal mucosa. 2,4) In order to develop the safe formulation that can improve the absorption of drugs categorized into BCS Class IV such as rebamipide, [14][15][16][17][18][19][20] we have compared the suppository that contains sodium laurate (C12) as an absorption enhancer and taurine (Tau) as a cytoprotective adjuvant with the commercial suppository that contains sodium caprate (C10) as an absorption enhancer. [21][22][23] We have found that C12, a mediumchain fatty acid salt, is a promising absorption enhancer and better than C10 in terms of the absorption improving ability, 24) and that Tau has the most effective cytoprotective activity among amino acids examined.…”

Evaluation of the antiulcerogenic activity profile of a flavonol diglucoside from Equisetum palustre L.

“…(Ishiyama et al, 1989) After fasting on water for 24 h, test samples were administered to rats orally 30 min before the surgical operation. The rats were then anesthetized and a midline laparotomy was performed to ligate the pylorus of each.…”

Reduction of the Extent of Diethyldithiocarbamate‐induced Antral Gastric Ulcer by Neutrophil Depletion in the Rat

“…It has been demonstrated that active oxygen species, 0 ; or .OH, may be involved in the pathogenesis of mucosal injury in the alimentary tract (Parks & Granger 1983;Perry et al 1986). We reported previously that diethyldithiocarbamate (DDC), and inhibitor of copper, zinc-superoxide dismutase (Cu, Zn-SOD), showed ulcerogenicity in the rat stomach, and that the effect was due to active oxygen species and gastric mucosal ischaemia (Ogino et al 1987(Ogino et al & 1990. However, these gastric lesions are multiple shallow ulcers, the bottoms of which do not reach the muscularis mucosa histologically.…”

“…DDC treatment failed to cause ulceration in some of the rats. After having treated the rats with DDC, pyloric ligation and intragastric application of 0.1 N HCI (Ishiyama et al 1988), we have succeeded in producing a large ulcer with destruction of the muscular layer in the antrum.…”