Gas-liquid chromatography of parathion metabolites as a sensitive index of induction of liver microsomal mixed-function oxidases

Analysis of phosphorus-containing hydrolytic products of organophosphorus insecticides in water

“…With one exception (Plapp and Casida, 1958), all available methods for analysis of ionic dialkyl phosphates involve their solvent partitioning from small, highly acidified volumes, usually less than 3 ml (St. John and Lisk, 1968; Askew et al, 1969;Shafik and Enos, 1969;Shafik et al, 1970Shafik et al, , 1971aShafik et al, ,b, 1973Thompson, 1974), or from solid crop samples (Archer, 1974). From our experience, the partitioning of DEP and especially DMP from small volumes of water is not a favorable process; using the extraction procedure of Shafik and Enos (1969), Davis et al (1973) recovered 75% of the DEP and 91% of the DETP from aqueous microsomal preparations after three successive extractions of a 2.5-ml sample. With large volumes of aqueous sample, efficient partitioning would require the use of very large quantities of expensive reagent grade solvents.…”

Pathway of nitro reduction of parathion by spinach homogenate

“…The present analytical method will be useful as a sensitive and selective assay method of nitro-reducing systems in various organisms. Gas-liquid chromatography of oxidative metabolites of parathion was also available as a sensitive and precise index of induction of liver microsomal drug-metabolizing enzymes (Davis et al, 1973).…”

Effects of diphenylhydantoin and chloroquine on monkey liver microsomal mixed‐function oxidases

“…All assays and procedures used for the monkey livers were as published previously (Davis et al, 1973) with the exception of the determination of cytochrome P-450 content of squirrel monkey microsomes and the glucuronyl transferase assay. The cytochrome P-450 assay was a modification of the method of Alvares et al (1968).…”