Gas chromatography mass spectrum and fourier-transform infrared spectroscopy analysis of methanolic extract of Rosmarinus oficinalis leaves

Hameed, Imad Hadi; Ibraheam, Israa Adnan; Kadhim, Hawraa Jawad

doi:10.5897/jpp2015.0348

Cited by 39 publications

(4 citation statements)

References 18 publications

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“…Interestingly, those bands can be an evidence of the presence of terpenes, such as camphor and eucalyptol, main compounds in R-EO used in the present work and already reported by several authors for rosemary EOs from Morocco (Hameed et al, 2015;Dzimitrowicz et al, 2019;El-harraf et al, 2020).…”

Section: Fouriertransforminfrared(ftir)spectroscopysupporting

confidence: 84%

Encapsulation of Rosmarinus officinalis essential oil in β‐cyclodextrins

Kharraf

Farah

Hadrami

et al. 2021

J. Food Process. Preserv.

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The aim of this study was to investigate the encapsulation process of R. officinalis (rosemary) essential oil (REO) in beta cyclodextrin (β-CD), by co-precipitation, using response surface methodology (RSM), specifically the face-centered central composite design (FCCCD). Solid-to-solid (β-CD/REO) and liquid-to-liquid (ethanol/water) ratios were selected as independent variables and their influence on the dependent variables was assessed. The recovered powder was characterized by different techniques, such as scanning electron microscope (SEM), Fourier Transform-Infrared Spectroscopy (FTIR) and X-Ray Diffractometry (XRD). Moreover, the release of REO from the β-CD complexes was investigated at different temperatures and pH values.The responses were affected by the concentration of ethanol in the reaction mixture and the amount of REO used. The optimal responses set by the RSM model (pvalue < 0.05) were obtained for the optimal parameters ranging from 1.25:1 to 1.7:1 and 16.25:83.75 to 17.5:82.5 for ethanol/water and β-CD/REO ratios, respectively.The size of the inclusion complexes from the experimental condition 1:1 ethanol/ water and 10:90 β-CD/REO ratios was the highest, with a value of 2.735 ± 0.192 µm.Furthermore, the FTIR spectra of inclusion complexes revealed a band downshift relative to the spectrum of β-CD, attributable to hydrogen bonding between REO components and β-CD, as well as a new band at 1,747 cm −1 , revealing the presence of oil components, while the XRD patterns of β-CD recorded before and after the inclusion process indicate a change from a cage-type packing to a channel-type packing of cyclodextrin, which, in turn, indicates a successful inclusion process. PracticalapplicationsThe encapsulation of EOs into cyclodextrins can enhance their biological properties, which include antioxidant, antimicrobial, anticarcinogenic, anti-inflammatory, and antidepressant activities. Nevertheless, a careful evaluation of the encapsulation conditions should be carried out in order to ensure the highest encapsulation yield without quality loss. In this way, pH and temperature, among other conditions, must be considered for evaluation since food or other matrices may present diverse pH values and be subjected to different temperatures. The present work may contribute to the use of rosemary EOs, with antioxidant and antimicrobial properties, in food and cosmetic industries, avoiding the sometimes-unpleasant flavor and aroma characteristics.

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Section: Fouriertransforminfrared(ftir)spectroscopysupporting

confidence: 84%

Encapsulation of Rosmarinus officinalis essential oil in β‐cyclodextrins

Kharraf

Farah

Hadrami

et al. 2021

J. Food Process. Preserv.

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“…In general, after AgNPs biosynthesis the decrease in all peak intensities was observed, and it can also be said that almost all main peaks remained at the same wavenumber positions. The FTIR spectra show broad hydrogen bonds at 3550–3200 cm −1 , which are assigned to O–H stretching in phenols and alcohol compounds [ 28 , 29 ]. Hydroxyl groups and C–H stretches (alkyl groups present in most organic molecules) were observed at ~2927 cm −1 [ 30 , 31 ].…”

Section: Resultsmentioning

confidence: 99%

Preparing, Characterization and Anti-Biofilm Activity of Polymer Fibers Doped by Green Synthesized AgNPs

2021

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The aim of the work was to prepare polymer matrix composite (PMC) microfibers doped by green synthesized silver nanoparticles (AgNPs). The incorporation of AgNP into the polymer matrix can provide toxic properties to the polymer. Polyvinyl alcohol (PVA) was used as a matrix. AgNPs were synthesized by the green method, where the leaf extract of Rosmarinus officinalis (R. officinalis) was used as a reduction and capping agent. PVA‑AgNPs composites were prepared in two ways: the ex situ method (pre‑prepared globular AgNPs with a mean diameter of 20 nm were added into polymer matrix) and the in situ method (AgNPs were synthesized in the process of polymer composite preparation; in situ synthesized nanoparticles were a mix of different shapes with a mean diameter of ~100 nm). FTIR (Infrared spectroscopy with Fourier Transformation), UV–vis (Ultraviolet–visible spectroscopy), TEM (Transmission Electron Microscope), EDX (Energy-dispersive X-ray spectroscopy), and SEM (Scanning Electron Microscope) techniques were used for the analysis of nanoparticles and prepared PMCs. Thin layers and microfibers of in situ and ex situ PMCs were prepared. The presence of AgNPs clusters was evident in both PMC thin layers. After electrospinning, the chains of nanoparticles were observed inside the fibers. The distribution of nanoparticles was improved by increasing the AgNPs volume fraction (from 5 vol.% to 20 vol.%). Toxic and antibiofilm activity of AgNPs colloid, pure PVA, and PVA‑AgNPs composites against the one-cell green algae Parachlorella kessleri (P. kessleri) was analyzed. AgNPs colloid, as well as PVA‑AgNPs composites, showed good toxic and antibiofilm activity, and pure PVA shows no toxic/antibiofilm activity.

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“…Based on those two major antigenic determinants several vaccines have been designed. [25] Klebsiella species are routinely found in the human nose, mouth, and gastrointestinal tract as normal flora; however, they can also behave as opportunistic human pathogens. Klebsiella species are known to also infect a variety of other animals, both as normal flora and opportunistic pathogens.…”

Section: Klebsiellamentioning

confidence: 99%

Characterization of antimicrobial secondary metabolites produced by Klebsiella pneumoniae and screening of its bioactive natural compounds using gas chromatography-mass spectrometry (GC-MS)

Sajjad Saad Zghair,

Sahar Sarhan Thajeel,

Imad Hadi Hameed

2023

Int. J. Life Sci. Res. Arch.

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Microbial secondary metabolites are low molecular mass products with unusual structures. The structurally diverse metabolites show a variety of biological activities like antimicrobial agents. Thirty nine bioactive compounds were identified in the methanolic extract of Klebsiella pneumoniae. GC-MS analysis of Klebsiella pneumoniae revealed the existence of the Tricyclo[4.3.1.1(3.8)]undecan-1-amine, 3-Methoxybenzaldehyde semicarbazone, carboxaldehyde , 1-methyl-,oxime ,(Z)-(+), 1,5,5-Trimethyl-6-methylene-cyclohexene, 4-(2,5-Dihydro-3-methoxyphenyl)butylamine, Paromomycin , 9-Borabicyclo[3.31]nonane , 9-mercapto-, Benzenemethanol , 2-(2-aminopropoxy)-3-methyl, Acetamide , N-(6-acetylaminobenzothiazol-2-yl)-2-(adamantan, rin-6-carboxylic acid , 4-(2,5-Dihydro-3-methoxyphenyl)butylamine, N-(2,5-Dicyano-3,4-dihydro-2H-pyrrol-2-yl)-acetamide, 3,10-Dioxatricyclo [4.3.1.0(2,4)]dec-7-ene, 3-Cyclohex-3-enyl-propionic acid, Eicosanoic acid ,phenylmethyl ester, 3,7-Diazabicyclo[3.3.1]nonane , 9,9-dimethyl-, Dithiocarbamate , S-methyl-,N-(2-methyl-3-oxobutyl)-, dl-Homocysteine, 2-(2-Furyl)pyridine, 1,7-Dioxa-10-thia-4,13-diazacyclopentadeca-5,9,12-trione, 5,7-Dodecadiyn-1,12-diol, 1-(β-d-Arabinofuranosyl)-4-O-difluoromethyluracil, Uric acid, Pyrrolo[1.2-a]pyrazine-1,4-dione , hexahydro-,12-Methyl-oxa-cyclododecan-2-one, Phthalic acid , butyl undecyl ester, 9,12,15-Octadecatrienoic acid , 2,3-bis(acetyloxy)propyl ester, 1,2,4-Trioxolane-2-octanoic acid 5-octyl-, methyl ester, 12-Dimethylamino-10-oxododecanoic acid , Octahydrochromen-2-one, L-Aspartic acid , N-glycyl-,2H-Oxecin-2-one , 3,4,7,8,91,10-hexahydro-4-hydroxy-10-meth , Thiazolo[4,5-d]pyrimidine-5,7(4H,6H)-dione , 2-amino-4-(2-ph, Dec-9-en-6-oxo-1-ylamide, 3,6,12-Trimethyl-1,4,7,10,13,16-hexaaza-cyclooctadecane, 2-lodohiistidine, 2,5-Piperazinedione ,3,6-bis(2-methylpropyl)-, 9-Octadecenamide , (Z)-, 3',8,8'-Trimethoxy-3-piperidyl-2,2'-binaphthalene-1,1',4,4'-tetra. Clinical pathogens selected for antibacterial activity namely, Streptococcus pneumonia, Escherichia coli, Staphylococcus aureus, Proteus mirabilis, Staphylococcus epidermidis, It were 4.09±0.013, 2.99±0.300, 4.37±0.200, 3.22±0.210, and 4.00±0.203 respectively for Bacterial products (Metabolites Produced by Klebsiella pneumoniae), while recorded 1.08±0.200, 0.97±0.116, 2.08±0.233, 3.04±0.261, 0.98±0.166 respectively for Bacterial products Streptomycin antibiotics, and recorded 1.02±0.180, 1.00±0.190, 2.08±0.236, 1.00±0.100, and 1.82±0.200 respectively for Kanamycin antibiotics. Klebsiella pneumoniae produce many important secondary metabolites with high biological activities. Based on the significance of employing bioactive compounds in pharmacy to produce drugs for the treatment of many diseases, the purification of compounds produced by Klebsiella pneumoniae can be useful.

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Gas chromatography mass spectrum and fourier-transform infrared spectroscopy analysis of methanolic extract of Rosmarinus oficinalis leaves

Cited by 39 publications

References 18 publications

Encapsulation of Rosmarinus officinalis essential oil in β‐cyclodextrins

Encapsulation of Rosmarinus officinalis essential oil in β‐cyclodextrins

Preparing, Characterization and Anti-Biofilm Activity of Polymer Fibers Doped by Green Synthesized AgNPs

Characterization of antimicrobial secondary metabolites produced by Klebsiella pneumoniae and screening of its bioactive natural compounds using gas chromatography-mass spectrometry (GC-MS)

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