Preparing, Characterization and Anti-Biofilm Activity of Polymer Fibers Doped by Green Synthesized AgNPs

Abstract

The aim of the work was to prepare polymer matrix composite (PMC) microfibers doped by green synthesized silver nanoparticles (AgNPs). The incorporation of AgNP into the polymer matrix can provide toxic properties to the polymer. Polyvinyl alcohol (PVA) was used as a matrix. AgNPs were synthesized by the green method, where the leaf extract of Rosmarinus officinalis (R. officinalis) was used as a reduction and capping agent. PVA‑AgNPs composites were prepared in two ways: the ex situ method (pre‑prepared globular AgNPs with a mean diameter of 20 nm were added into polymer matrix) and the in situ method (AgNPs were synthesized in the process of polymer composite preparation; in situ synthesized nanoparticles were a mix of different shapes with a mean diameter of ~100 nm). FTIR (Infrared spectroscopy with Fourier Transformation), UV–vis (Ultraviolet–visible spectroscopy), TEM (Transmission Electron Microscope), EDX (Energy-dispersive X-ray spectroscopy), and SEM (Scanning Electron Microscope) techniques were used for the analysis of nanoparticles and prepared PMCs. Thin layers and microfibers of in situ and ex situ PMCs were prepared. The presence of AgNPs clusters was evident in both PMC thin layers. After electrospinning, the chains of nanoparticles were observed inside the fibers. The distribution of nanoparticles was improved by increasing the AgNPs volume fraction (from 5 vol.% to 20 vol.%). Toxic and antibiofilm activity of AgNPs colloid, pure PVA, and PVA‑AgNPs composites against the one-cell green algae Parachlorella kessleri (P. kessleri) was analyzed. AgNPs colloid, as well as PVA‑AgNPs composites, showed good toxic and antibiofilm activity, and pure PVA shows no toxic/antibiofilm activity.

An Eco-Friendly Approach to the Control of Pathogenic Microbes and Anopheles stephensi Malarial Vector Using Magnesium Oxide Nanoparticles (Mg-NPs) Fabricated by Penicillium chrysogenum

“…Therefore, researchers’ attention is directed to the green method or biological method using bacteria, fungi, actinomycetes, algae, yeast, and plants to fabricate nanomaterials [ 7 , 8 , 9 , 10 , 11 ]. Recently, there is a huge number of nanomaterials being fabricated using biological methods, such as Ag, Au, Cu, CuO, ZnO, Fe 2 O 3 , TiO 2 , and others [ 12 , 13 , 14 , 15 , 16 , 17 ].…”

Hydroxyapatite Obtained via the Wet Precipitation Method and PVP/PVA Matrix as Components of Polymer-Ceramic Composites for Biomedical Applications

“…Despite of the fact that composite materials show many advantages, the possibility of the implant rejection due to the numerous bacterial infections still constitutes a problematic issue [ 30 ]. One of the most promising solutions seems to be the development of the composite materials modified with appropriate substances such as, e.g., silver nanoparticles showing antibacterial properties [ 31 ].…”

Antibacterial and Antifungal Studies of Biosynthesized Silver Nanoparticles against Plant Parasitic Nematode Meloidogyne incognita, Plant Pathogens Ralstonia solanacearum and Fusarium oxysporum

“…Plant extract of R. officinalis, U. dioica, and V. vitis-idaea were used for AgNPs synthesis [9]. Extract of alga Parachlorella kessleri can be used for AgNPs synthesis and they have antimicrobial potential [10,11]. Green synthesis of nanomaterials is an important alternative tool to reduce the destructive effects associated with the traditional methods of synthesis for nanoparticles commonly utilized in laboratory and industry settings [12][13][14][15].…”