Gas chromatography–mass spectrometry with tert.-butyldimethylsilyl derivatization: use of the simplified sample preparations and the automated data system to screen for organic acidemias

Ohie, Takaharu; Fu, Xiaowei; Iga, Misako; Kimura, Masahiko; Yamaguchi, Seiji

doi:10.1016/s0378-4347(00)00105-5

Cited by 32 publications

(14 citation statements)

References 11 publications

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“…The GC interface and source temperatures were set to 250°C, and electron impact (EI ϩ ) mass spectra were acquired at 70 eV from 0 to 47 min with an acquisition rate of 1 spectrum/s. Chromatographic peaks were identified either from existing mass spectral and retention time data from standards previously analyzed at Rothamsted Research, Ltd. (Harpenden Herts, United Kingdom), or from the NIST mass spectral database in conjunction with retention data obtained from the literature (30). Determination of the accurate mass, to within 5 ppm, of molecular ion M ϩ and fragmentation ions M-15 ϩ and M-57 ϩ was used to verify analyte identifications.…”

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confidence: 99%

Improvement of Phosphate Solubilization and Medicago Plant Yield by an Indole-3-Acetic Acid-Overproducing Strain of Sinorhizobium meliloti

Bianco

Defez

2010

Appl Environ Microbiol

132

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Nitrogen (N) and phosphorus (P) are the most limiting factors for plant growth. Some microorganisms improve the uptake and availability of N and P, minimizing chemical fertilizer dependence. It has been published that the RD64 strain, a Sinorhizobium meliloti 1021 strain engineered to overproduce indole-3-acetic acid (IAA), showed improved nitrogen fixation ability compared to the wild-type 1021 strain. Here, we present data showing that RD64 is also highly effective in mobilizing P from insoluble sources, such as phosphate rock (PR). Under P-limiting conditions, the higher level of P-mobilizing activity of RD64 than of the 1021 wild-type strain is connected with the upregulation of genes coding for the high-affinity P transport system, the induction of acid phosphatase activity, and the increased secretion into the growth medium of malic, succinic, and fumaric acids. Medicago truncatula plants nodulated by RD64 (Mt-RD64), when grown under P-deficient conditions, released larger amounts of another P-solubilizing organic acid, 2-hydroxyglutaric acid, than plants nodulated by the wild-type strain (Mt-1021). It has already been shown that Mt-RD64 plants exhibited higher levels of dry-weight production than Mt-1021 plants. Here, we also report that P-starved Mt-RD64 plants show significant increases in both shoot and root fresh weights when compared to P-starved Mt-1021 plants. We discuss how, in a Rhizobium-legume model system, a balanced interplay of different factors linked to bacterial IAA overproduction rather than IAA production per se stimulates plant growth under stressful environmental conditions and, in particular, under P starvation.

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confidence: 99%

Improvement of Phosphate Solubilization and Medicago Plant Yield by an Indole-3-Acetic Acid-Overproducing Strain of Sinorhizobium meliloti

Bianco

Defez

2010

Appl Environ Microbiol

132

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“…These results suggest that the solvent extraction and purification on the cation ion-exchange resins were appropriate to selectively separate the amino acids from sample contaminants, and these pre-analysis steps resulted in good chromatographic separation. This method reduces GC/MS analysis time by one-third to one-sixth compared to the conventional method (30-60 min) while retaining good chromatographic separation [9,20,31]. a Total values of Leu (Leucine) and Ile (Isoluecine).…”

Section: Discussionmentioning

confidence: 99%

“…If organic solvent treatment is used for the removal of proteins, the sample goes directly to derivatization following the drying procedure [5,8,[15][16][17]. For the derivatization procedure, trimethylsilylation [4,18,19], tert-butyldimethylsilylation [20,21], esterifcation-acylation [5,16], and alkyl chloroformation [8,9,[22][23][24][25] have been reported and those methods were summarized by Knapp [26] and Blau and Halket [27]. For quantitative calculation, the absolute calibration method is widely used but the isotope dilution method was used to improve the accuracy [10,17,28].…”

Section: Introductionmentioning

confidence: 99%

Simple and rapid analytical method for detection of amino acids in blood using blood spot on filter paper, fast-GC/MS and isotope dilution technique

Kawana

Nakagawa

Hasegawa

et al. 2010

Journal of Chromatography B

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“…However, in the GC/MS metabolome analysis of urine, trimethylsilylation may be better (Kuhara, 2001). Although t-butyldimethylsilylation give rise to an intense (M-57) signal, incomplete derivatization for polyols such as glycerol, erythritol, or hexoses is reported (Ohie et al, 2000;Fiehn et al, 2002). Because the t-butyldimethylsilyl moiety is bulkier than the trimethylsilyl moiety, the tendency not to be fully silylated, and to give several derivatives may be higher for t-butyldimethylsilylation, especially for polyols and sugars.…”

Section: Gc-ms Analysis Of Inborn Errors Of Metabolismmentioning

confidence: 99%

Gas chromatographic–mass spectrometric urinary metabolome analysis to study mutations of inborn errors of metabolism

Kuhara

2004

Mass Spectrometry Reviews

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Urine contains numerous metabolites, and can provide evidence for the screening or molecular diagnosis of many inborn errors of metabolism (IEMs). The metabolomic analysis of urine by the combined use of urease pretreatment, stable-isotope dilution, and capillary gas chromatography/mass spectrometry offers reliable and quantitative data for the simultaneous screening or molecular diagnosis of more than 130 IEMs. Those IEMs include hyperammonemias and lactic acidemias, and the IEMs of amino acids, pyrimidines, purines, carbohydrates, and others including primary hyperoxalurias, hereditary fructose intolerance, propionic acidemia, and methylmalonic acidemia. Metabolite analysis is comprehensive for mutant genotypes. Enzyme dysfunction-either by the abnormal structure of an enzyme/apoenzyme, the reduced quantity of a normal enzyme/apoenzyme, or the lack of a coenzyme-is involved. Enzyme dysfunction-either by an abnormal regulatory gene, abnormal sub-cellular localization, or by abnormal post-transcriptional or post-translational modification-is included. Mutations-either known or unknown, common or uncommon-are involved. If the urine metabolome approach can accurately observe quantitative abnormality for hundreds of metabolites, reflecting 100 different disease-causing reactions in a body, then it is possible to simultaneously detect different mutant genotypes of far more than tens of thousands.

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Gas chromatography–mass spectrometry with tert.-butyldimethylsilyl derivatization: use of the simplified sample preparations and the automated data system to screen for organic acidemias

Cited by 32 publications

References 11 publications

Improvement of Phosphate Solubilization and Medicago Plant Yield by an Indole-3-Acetic Acid-Overproducing Strain of Sinorhizobium meliloti

Improvement of Phosphate Solubilization and Medicago Plant Yield by an Indole-3-Acetic Acid-Overproducing Strain of Sinorhizobium meliloti

Simple and rapid analytical method for detection of amino acids in blood using blood spot on filter paper, fast-GC/MS and isotope dilution technique

Gas chromatographic–mass spectrometric urinary metabolome analysis to study mutations of inborn errors of metabolism

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