Gas chromatography–mass spectrometry method for determination of phenylalanine and tyrosine in neonatal blood spots

Deng, Chunhui; Deng, Yonghui; Wang, Bin; Yang, Xinying

doi:10.1016/s1570-0232(02)00632-3

Cited by 98 publications

(46 citation statements)

References 23 publications

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“…20,21 It can seen from Table 2 that the ratio of Phe to Tyr in PKU-positive blood samples was much higher than that in normal blood samples. Therefore, simultaneous screening for MSUD and PKU could be achieved by analysis of the five amino acids in neonatal blood samples using the proposed method.…”

Section: Determination Of Amino Acids In Neonatal Blood Spot Samplesmentioning

confidence: 93%

“…In our previous studies, we demonstrated the feasibility of diagnosis of PKU and MSUD using GC/MS analysis of amino acids in neonatal blood samples. 20,21 In the present method, isobutyl chloroformate was introduced for derivatization of amino acids in blood samples. Moreover, a simple and rapid sample SPME technique was developed for extraction of the amino acid derivatives.…”

Section: Detection Limit Recovery and Precisionmentioning

confidence: 99%

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Rapid determination of amino acids in neonatal blood samples based on derivatization with isobutyl chloroformate followed by solid‐phase microextraction and gas chromatography/mass spectrometry

Deng

Zhang

2004

Rapid Comm Mass Spectrometry

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The purpose of this study was to develop a simple, rapid and sensitive analytical method for determination of amino acids in neonatal blood samples. The developed method involves the employment of derivatization and a solid-phase microextraction (SPME) technique together with gas chromatography/mass spectrometry (GC/MS). Amino acids in blood samples were derivatized by a mixture of isobutyl chloroformate, methanol and pyridine, and the N(O,S)-alkoxycarbonyl alkyl esters thus formed were headspace extracted by a SPME fiber. Finally, the extracted analytes on the fiber were desorbed and detected by GC/MS in electron impact (EI) mode. L-Valine, L-leucine, L-isoleucine, L-phenylanaline and L-tyrosine in blood samples were quantitatively analyzed by measurement of the corresponding N(O,S)-alkoxycarbonyl alkyl esters using an external standard method. SPME conditions were optimized, and the method was validated. The method was applied to diagnosis of neonatal phenylkenuria (PKU) and maple syrup urine disease (MSUD) by the analyses of five amino acids in blood samples. The results showed that the proposed method is a potentially powerful tool for simultaneous screening for neonatal PKU and MSUD.

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Section: Determination Of Amino Acids In Neonatal Blood Spot Samplesmentioning

confidence: 93%

Section: Detection Limit Recovery and Precisionmentioning

confidence: 99%

Rapid determination of amino acids in neonatal blood samples based on derivatization with isobutyl chloroformate followed by solid‐phase microextraction and gas chromatography/mass spectrometry

Deng

Zhang

2004

Rapid Comm Mass Spectrometry

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“…Since the product of urine concentration and urine flow rate yields creatinine excretion rate, which is the rate of removal from the blood, creatinine clearance is calculated as removal rate per min (Cr ur ×υ) divided by the plasma creatinine concentration (Cr pl ). (National Kidney Foundation, 2002) [21], which mathematically is expressed as C cr = (Cr ur ×F)/Cr pl (mL/min)…...Eq 2…”

Section: Estimated Glomerular Filtration Rate (Egfr)mentioning

confidence: 99%

Therapeutic effect of amino acid mixture on type 1 diabetes mellitus with impaired renal methionine reabsorption

Peng¹,

Ker²,

Hsieh³

et al. 2014

J Diab Res Clin Met

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Background: The main manifestation of Type 1 diabetes mellitus (T1DM) is insulin insufficiency which eventually leads to body weight loss and a diversity of organ dysfunctions. On the other hand, quercetin (QT) is an antioxidant and an insulin secretagogue. We hypothesize that the amino acid mixture (AAM) preparation and/or AAM+ quercetin(QT) probably could ameliorate these adverse effects. Methods: The STZ-DM-Sprague Dawley rat model was carried out and respectively treated with QT, AAM, and AAM+QT. The relevant physiological and biochemical changes in serum and urinary parameters were examined. Results: T1DM exhibited severe insulin insufficiency, body weight loss, increased kidney/body weight ratio, BUN, creatinine clearance, albuminuria and proteinuria, declined serum albumin and amino acid reabsorption involving methionine, leucine and isoleucine. The control showed severe serinuria (12.0±0.2 mg/mL) (p<0.001). AAM caused valinuria (3.6±0.2 mg/mL), argininuria (6.9±0.2 mg/mL) and histidinuria (6.5±0.1 mg/mL) (p<0.05). T1DM rats revealed hyperglycinuria (21.4±0.2 mg/mL) (p<0.01); AAM alleviated hyperglycinuria (13.7±0.2 mg/mL) (p<0.001) but evoked isoleucinuria (8.2±0.2 mg/mL) (p<0.05); QT elicited methioninuria (23.1±0.3 mg/mL) in T1DM and the control (p<0.001); AAM+QT alleviated the methioninuria with extra leucinuria (11.3±0.4 mg/mL) (p<0.05), isoleucinuria (11.4±0.4 mg/mL), tryptophanuria, argininuria and lysinuria (p<0.05). Conclusion: T1DM exhibits severe insulin insufficiency, resulting in severe body weight loss and increased kidney/body weight ratio. T1DM and QT tend to induce methioninineuria. AAM+QT can rescue methioninuria at the expense of leucinuria, isoleucinuria, tryptophanuria, argininuria and lysinuria, implicating the use of AAM with extra supplement of insulin, leucine, isoleucine, tryptophan, arginine and lysine is feasible for alleviation of T1DM.

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“…The assay of Tyr is required in clinic and food fields and so on. The approaches for Tyr determination are mainly high-performance liquid chromatography [3], chromatography-mass spectrometry [4,5], and capillary electrophoresis [6,7]. However, these methods suffer from either slow detection speed or high expense.…”

Section: Introductionmentioning

confidence: 99%

Improved Voltammetric Response of L‐Tyrosine on Multiwalled Carbon Nanotubes‐Ionic Liquid Composite Coated Glassy Electrodes in the Presence of Cupric Ion

et al. 2008

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l-Tyrosine can exhibit a small anodic peak on multiwalled carbon nanotubes (MWCNTs) coated glassy carbon electrodes (GCE). At pH 5.5 its peak potential is 0.70 V (vs. SCE). When an ionic liquid (i.e., 1-octyl-3-methylimidazolium hexafluorophosphate, [omim][PF 6 ]) is introduced on the MWCNT coat, the peak becomes bigger. Furthermore, in the presence of Cu 2þ ion the anodic peak of l-tyrosine increases further due to the formation of Cu 2þ -l-tyrosine complex, while the peak potential keeps unchanged. Therefore, a sensitive voltammetry based on the oxidation of Cu 2þ -l-tyrosine complex on MWCNTs-[omim] [PF 6 ] composite coated electrode is developed for ltyrosine. Under the optimized conditions, the anodic peak current is linear to l-tyrosine concentration in the range of 1 Â 10 À8 -5 Â 10 À6 M, and the detection limit is 8 Â 10 À9 M. The modified electrode shows good reproducibility and stability. In addition, the voltammetric behavior of other amino acids is explored. It is found that among them tryptophan (Trp) and histidine (His) can also produce sensitive anodic peak under same experimental conditions, and their detection limits are 4 Â 10 À9 M and 4 Â 10 À6 M, respectively.

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Gas chromatography–mass spectrometry method for determination of phenylalanine and tyrosine in neonatal blood spots

Cited by 98 publications

References 23 publications

Rapid determination of amino acids in neonatal blood samples based on derivatization with isobutyl chloroformate followed by solid‐phase microextraction and gas chromatography/mass spectrometry

Rapid determination of amino acids in neonatal blood samples based on derivatization with isobutyl chloroformate followed by solid‐phase microextraction and gas chromatography/mass spectrometry

Therapeutic effect of amino acid mixture on type 1 diabetes mellitus with impaired renal methionine reabsorption

Improved Voltammetric Response of L‐Tyrosine on Multiwalled Carbon Nanotubes‐Ionic Liquid Composite Coated Glassy Electrodes in the Presence of Cupric Ion

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