Gas chromatographic separation and analysis of trimethylsilyl derivatives of some naturally occurring non-volatile phenolic compounds and related substances

Casteele, Karel Vande; Pooter, H. De; Sumere, C. F. Van

doi:10.1016/s0021-9673(00)82297-9

Cited by 57 publications

(13 citation statements)

References 34 publications

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“…The phenolic acids remaining were then converted to trimethylsilyl derivatives and analyzed on a 200 cm by 2 mm i.d. glass column filled with 3 % OV-25 coated on a 60-80 mesh Chromosorb W support installed in a Varian 3700 gas chromatograph fitted with a flame ionization detector (Vande Casteele et al 1976). The column was temperature programmed from 100 to 260 "C at 6 C" min-l with an initial hold of 5 min.…”

Section: Methodsmentioning

confidence: 99%

Decomposition in salt marsh ecosystems: phenolic dynamics during decay of litter of Spartina alterniflora

Wilson¹,

Buchsbaum²,

Valiela³

et al. 1986

Mar. Ecol. Prog. Ser.

100

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Loss of weight and change in concentration of soluble phenolics, bound phenolics, lignins, and cell wall material were followed over 2 yr of decay in 4 types of litter of Spartina alterniflora. The pattern of weight loss showed rapid leaching during the first month, temperaturedependent decay during the first year, and little further loss during the second year. Soluble phenolics were lost throughout decomposition. Initially some soluble phenolics may become attached to cell walls; absolute increases in bound phenolics were observed in low marsh litters during the first 2 wk of decay. Lignins were lost at the slowest rate from litter material and increased in relative concentration during decay. Intraspecific differences in litter quality were small, and did not alter the pattern of decomposition among the 4 types of litter. Changes in chemical composition of litter during decay, however, were major controls of decay rates during the second and third phases of decomposition. Decreasing weight loss correlated with increasing lignin and nitrogen content. The increase in nitrogen presumably reflected synthesis of refractory nitrogen-enriched complexes. As decay continued, the litter became progressively enriched in recalcitrant lignin and nitrogen-enriched complexes and little further loss of organic matter occurred.

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Section: Methodsmentioning

confidence: 99%

Decomposition in salt marsh ecosystems: phenolic dynamics during decay of litter of Spartina alterniflora

Wilson¹,

Buchsbaum²,

Valiela³

et al. 1986

Mar. Ecol. Prog. Ser.

100

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show abstract

“…In all cases, the final temperature was kept for 10 min. All the samples analyzed were derivatized with BSTFA prior to injection (adapted from Casteele et al, 1976;Popova et al, 2005). The compounds were characterized by mass spectral interpretation and automatic comparison with a spectra library, and, if available, reference compounds were co-chromatographed in order to confirm the retention times.…”

Section: Hrgc-ms Analysismentioning

confidence: 99%

Evaluation of Antioxidant and Antimicrobial Activities and Characterization of Bioactive Components of Two Brazilian Propolis Samples Using a pK_a-Guided Fractionation

Souza

Patitucci

et al. 2007

Zeitschrift Für Naturforschung C

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The ethanolic extracts of two Brazilian propolis samples were submitted to a fractionation procedure based on the pK a values of their components. The fractions obtained were evaluated for their antimicrobial activity against Staphylococcus aureus as well as for their antioxidant properties (reduction of DPPH radical). Their phenolic and flavonoid contents were measured spectrophotometrically, in order to establish the correlations between these contents and the measured activities. Further, the most active fractions of both extracts were analyzed by HRGC-MS and about twenty compounds could be characterized. Among them were 3-prenyl-4-hydroxycinnamic acid (drupanin) and 3,5-diprenyl-4-hydroxycinnamic acid (artepillin C), which seem to be the major antioxidant components of the bioactive fractions.

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“…Phenolic acids were separated and determined by a gas chromatograph equipped with FID according to the method of Casteele (5).…”

Section: Phenolic Compound Determination and Gas Chromatographic Sepamentioning

confidence: 99%

Browning Mechanism of Water Convolvulus (Ipomoea aquativa Forsk.) Stored at Low Temperature

Ose

Chachin

Imahori

1995

ACS Symposium Series

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The young leaves and stems of water convolvulus, a leafy vegetable that originates in tropical regions, showed browning as a symptom of chilling injury at temperatures below 9 °C. The main compound associated with browning in this vegetable was found to be chlorogenic acid, synthesized by caffeic acid:CoA ligase (CL) and caffeoyl CoA:quinate hydroxycinnamoyl transferase (CQT). The activity of CQT was higher at 1 °C than at 20 °C. Three phenolic fractions, free, esterified and bound, were extracted from water convolvulus. At 1 °C, a part of the bound form was transformed into a free reactive form. Electron-dense deposits produced by action of polyphenoloxidase on DOPA were observed with electron microscopy. These deposits were only seen in grana-and stomathylakoids. Chloroplast structure in the mesophyll cells was destroyed before the appearance of chilling injury. Three fractions: F-I, chloroplast rich precipitate (300~3,000Xg); F-II, a precipitate (3,000~100,000Xg) and F-III, supernatant (100,000Xg) were obtained by centrifugation. The activities of CQT and PPO in F-I increased, reaching a maximum just before the occurrence of chilling injury.A great deal of attention has been focused on browning associated with chilling injury of fruits and vegetables during low temperature storage. In water convolvulus, an important leafy vegetable located in southeast Asia and southern Japan, browning was observed in the young leaves and stems during low temperature storage. In higher plants, phenolic acids consist of free and bound compounds and the free form is more reactive in browning than the bound one (7, 2). Shomer et al. (3) reported that polyphenoloxidase (PPO) is localized in the chloroplast thylakoid membrane in olive fruit. The objectives of our studies were to identify the levels and precursors of browning, changes in organization of the cells, the cellular localization of CQT and PPO in water convolvulus stored at low temperature, and to examine the mechanism of browning associated with chilling injury.

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Gas chromatographic separation and analysis of trimethylsilyl derivatives of some naturally occurring non-volatile phenolic compounds and related substances

Cited by 57 publications

References 34 publications

Decomposition in salt marsh ecosystems: phenolic dynamics during decay of litter of Spartina alterniflora

Decomposition in salt marsh ecosystems: phenolic dynamics during decay of litter of Spartina alterniflora

Evaluation of Antioxidant and Antimicrobial Activities and Characterization of Bioactive Components of Two Brazilian Propolis Samples Using a pK_a-Guided Fractionation

Browning Mechanism of Water Convolvulus (Ipomoea aquativa Forsk.) Stored at Low Temperature

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Gas chromatographic separation and analysis of trimethylsilyl derivatives of some naturally occurring non-volatile phenolic compounds and related substances

Cited by 57 publications

References 34 publications

Decomposition in salt marsh ecosystems: phenolic dynamics during decay of litter of Spartina alterniflora

Decomposition in salt marsh ecosystems: phenolic dynamics during decay of litter of Spartina alterniflora

Evaluation of Antioxidant and Antimicrobial Activities and Characterization of Bioactive Components of Two Brazilian Propolis Samples Using a pKa-Guided Fractionation

Browning Mechanism of Water Convolvulus (Ipomoea aquativa Forsk.) Stored at Low Temperature

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Evaluation of Antioxidant and Antimicrobial Activities and Characterization of Bioactive Components of Two Brazilian Propolis Samples Using a pK_a-Guided Fractionation