Gas chromatographic-mass spectrometric isotope dilution determination of N,N-dimethyltryptamine concentrations in normals and psychiatric patients

A critical review of reports of endogenous psychedelic N, N‐dimethyltryptamines in humans: 1955–2010

“…Walker et al [36] and Wyatt et al [37] employed an isotope dilution method monitoring two ions to detect and quantitate DMT in blood. Walker et al [36] and Wyatt et al [37] employed an isotope dilution method monitoring two ions to detect and quantitate DMT in blood.…”

“…Takesada et al [14] HDMT 24 hour urine Ext, Dowex 50 column, alumina column 1966 Runge et al [15] HDMT 1 L of urine pH 8-9, butanol ext, acetone ppt, acetone 1966 Perry et al [16] DMT, HDMT Heller [17] HDMT 1 L of urine NaHCO3 sat., butanol, evap, acetone 1967 Fischer and Spatz [18] HDMT 100 ml fresh urine NaCO3, ether ext, evap, acetone 1967 Kakimoto et al [19] DMT, HDMT 24 hour urine; vol 600 mg creatinine analyzed Ext, Dowex 50 column, alumina column 1967 Tanimukai [20] HNMT, HDMT, NMT, DMT, MDMT Fischer et al [33] HDMT, glucuronide 50 ml morning urine; w/wo glucuronidase Liquid-Liquid ext; w/wo glucuronidase treatment 1972 Himwich et al [34] HDMT, DMT, MDMT Narasimhachari et al [35] HDMT, DMT, MDMT 24 hour urine Walker et al [36] DMT plasma; DMT stable for 60 days at 6 degrees C HCL ext acid pH with CHCl3, pH 9, ext CHCl3, evap Wyatt et al [37] DMT plasma HCL ext acid pH with CHCl3, pH 9, ext CHCl3, evap 1973 Narasimhachari and Himwich [38] DMT, HDMT Lipinski et al [39] DMT plasma separated by centrifugation HCL ext acid pH with CHCl3, pH 9, ext CHCl3, evap 1974 Bidder et al [40] DMT Heparinised plasma or whole blood; 24 hr urine HCL ext acid pH with CHCl3, pH 9, ext CHCl3, evap 1974 Narasimhachari et al [41] HDMT, DMT, MDMT Forsstrom et al [67] DMT, MDMT, HDMT, NMT morning and afternoon urines; 5 ml assayed urine centrifuged and ext on Oasis SPE cartridge 2005 Karkkainen et al [68] DMT, HDMT urine (5 ml), plasma or serum (1 ml), stool;…”

“…Tanimukai et al [29] HNMT Wyatt et al [37] DMT plasma HCL ext acid pH with CHCl3, pH 9, ext CHCl3, evap 1973 Narasimhachari and Himwich [38] DMT, HDMT Lipinski et al [39] DMT plasma separated by centrifugation HCL ext acid pH with CHCl3, pH 9, ext CHCl3, evap 1974 Bidder et al [40] DMT Heparinised plasma or whole blood; 24 hr urine HCL ext acid pH with CHCl3, pH 9, ext CHCl3, evap 1974 Narasimhachari et al [41] HDMT Forsstrom et al [67] DMT, MDMT, HDMT, NMT morning and afternoon urines; 5 ml assayed urine centrifuged and ext on Oasis SPE cartridge 2005 Karkkainen et al [68] DMT, HDMT urine (5 ml), plasma or serum (1 ml), stool;…”

A Review of Nicotinic Acid, N-Methylated Indoleamines and Schizophrenia

“…Narasimhachari et al (1971b) found DMT in the plasma of 15 of 22 acute schizophrenics but only in 2 of 20 nonschizophrenics. Using a more specific method, Wyatt et al (1973a) could find no DMT in the plasma of schizophrenics, but found it in one normal and one depressive psychotic. Lipinski (1974) found detectable levels of DMT in the plasma of 2 of 11 schizophrenics, but none in chronic schizophrenics and the control groups.…”

Urinary Excretion of N,N-Dimethylated Tryptamines in Chronic Schizophrenia: A Review of the Present Status of the Hypothesis

“…N,Ndimethyl-tryptamine (DMT) is a hallucinogenic metabolite of serotonin and is a N-methyl derivative of tryptamine. Wyatt et al (40) have summarized the evidence for assuming that DMT might be the schizophreno genic hallucinogen. Hallucinogens may thus be a-methyl or N-methyl derivatives of cerebral metabolites, noradrenaline, dopamine or serotonin.…”