Gas chromatographic determination of pentazocine in human plasma by means of electron-capture detection

Brötell, Harald; Ehrsson, Hans; Gyllenhaal, Olle

doi:10.1016/s0021-9673(73)30080-2

Cited by 53 publications

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“…In DSC measurements, large quantities of experimental data can easily be collected using an automatic data acquisition system. However, the interpretation of such experimental data is not straightforward which is apparent from the literature where one can find considerable discrepancies among results reported (1)(2)(3). Many papers are devoted to an analysis of possible causes of these discrepancies.…”

Section: Resultsmentioning

confidence: 95%

“…This recovery was taken as quantitative for the following reasons. Derivatization by extractive alkylation has been shown to he quantitative for a variety of substrates and alkylating agents (7,2,4,19,23). When quantitative recovery, as determined by the above definition, did not occur, it was possible under the described experimental conditions to detect the parent phenol.…”

Section: Resultsmentioning

confidence: 99%

“…Pentafluorobenzylation has been used to develop highly sensitive methods for the quantitative determination of organic compounds in biological matrices (1)(2)(3)(4)(5)(6). Recently, Davis has described some novel conditions for the pentafluorobenzylation of carboxylic acids and phenols, described the gas chromatographic properties of the derivatives, and discussed the mechanisms involved in the preparation of the pentafluorobenzyl derivatives of these compounds (7).…”

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confidence: 99%

“…Most methods for quantitative determination of an organic compound in a biological matrix that use pentafluorobenzylation involve extractive alkylation in a biphasic system (1)(2)(3)(4). Extractive alkylation and phase transfer catalysis have been extensively reported as useful methods in preparative organic chemistry (8)(9)(10) and the mechanism of action of phase transfer catalysis has also been studied (11)(12)(13)(14).…”

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confidence: 99%

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Specific pentafluorobenzylation of phenols in a biphasic system

Rosenfeld¹,

Crocco²

1978

Anal. Chem.

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We thank the U.S. National Science Foundation (NSF Grant No. ENV 75-20802), the U.S. National Cancer Institute (NCI Contract No. NOI-CP-45623), the U.S. Environmental Protection Agency (EPA Contract No. 68-02-2363 and 68-02-2312), and the U.S. National Institute for Occupational Safety and Health (NIOSH for financial support. Any opinions, findings, conclusions, or recommendations expressed in this paper are those of the authors, and do not necessarily reflect the view's of the NSF, NCI, EPA, and/or NIOSH.

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Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Specific pentafluorobenzylation of phenols in a biphasic system

Rosenfeld¹,

Crocco²

1978

Anal. Chem.

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“…Electron Capture Response and Its Dependence on the Detector Temperature. The electron capture response of different pentafluorobenzyl and pentafluorobenzoyl derivatives is extremely high and enables the detection of picogram amounts of an actual compound (5)(6)(7)(8). The response of pentafluorobenzyl carbamates formed from different tertiary amines are in the order of 10~16 mol sec-1 which means that down to 3 pg can be detected (1, 2).…”

Section: Resultsmentioning

confidence: 99%

Electron capture gas chromatography of tertiary amines as pentafluorobenzyl carbamates

Hartvig¹,

W²,

Vessman³

et al. 1976

Anal. Chem.

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procedure has two disadvantages. One, the amount of derivative is about 10 times less than the amount detected by the procedure in reference 7 because of the limited amount of ethanol. Two, the resolution of the ethyl lactate heptafluorobutyrate peak from the trichloroethyl heptafluorobutyrate peak is extremely poor. A much better method for the EC-GLC determination of lactic acid is presented in a previous paper (7). The trichloroethyl ester of formic acid is also very poorly resolved from the trichloroethyl heptafluorobutyrate. Although the application of this procedure to other types of carboxylic acids presents problems, it has been demonstrated in this paper that it is a simple and sensitive EC-GLC method for detecting many of the short chain acids in spent culture media and in body fluids.

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HPLC separation of pentazocine enantiomers in serum using an ovomucoid chiral stationary phase

Kelly

Stewart

Blanton

1994

Biomedical Chromatography

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A stereospecific HPLC method was developed for the analysis of (-) and (+) pentazocine in human serum. Each enantiomer and the internal standard nalophine were isolated from serum using a liquid-liquid extraction procedure. Recoveries of 99.05 +/- 5.37 and 97.42 +/- 2.78% were obtained for (-) and (+) pentazocine, respectively. Resolution of the enantiomers was obtained by using an ovomucoid chiral stationary phase with a mobile phase of methanol:acetonitrile: 10 mM phosphate buffer, pH 5.8 (20:5.3:74.7 v/v/v). A resolution (Rs) value of 1.80 was obtained for the pentazocine enantiomers. Linear calibration curves were obtained in the 10-100 ng/mL range for each enantiomer in serum. The detection limit based on a signal-to-noise ratio of 3 was 5 ng/mL for each enantiomer in serum using fluorescence detection with excitation at 275 nm and emission set at 335 nm. The lowest quantifiable level was found to be 10 ng for each enantiomer. Precision and accuracy of the method were in the 3.8-4.8% and 1.3-4.2% ranges, respectively.

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Gas chromatographic determination of pentazocine in human plasma by means of electron-capture detection

Cited by 53 publications

References 0 publications

Specific pentafluorobenzylation of phenols in a biphasic system

Specific pentafluorobenzylation of phenols in a biphasic system

Electron capture gas chromatography of tertiary amines as pentafluorobenzyl carbamates

HPLC separation of pentazocine enantiomers in serum using an ovomucoid chiral stationary phase

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