Gas-chromatographic Analysis of the Fatty Acid Composition of Human Fatty Liver

Tanaka, Kay

doi:10.1093/oxfordjournals.jbchem.a127362

Cited by 14 publications

(9 citation statements)

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“…The fatty acid distribution in the triglyceride fractions differed significantly from that in the fatty acid fractions. The predominance of unsaturated fatty acids in the triglycerides is consistent with earlier observations (14)(15)(16)(17). In contrast, saturated fatty acids predominated in the free fatty acid fractions.…”

Section: Discussionsupporting

confidence: 92%

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Hepatic Free Fatty Acids in Alcoholic Liver Disease and Morbid Obesity

et al. 2007

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Alcoholic liver disease is characterized by the accumulation of fat and inflammatory changes in the liver. Because free fatty acids, the precursors of triglycerides, can damage biological membranes, accumulation of free fatty acids in the liver might be in part responsible for the functional and morphological changes seen in alcoholic liver disease. We, therefore, determined the hepatic lipid composition in biopsies from 31 patients with alcoholic liver disease, 18 patients with morbid obesity, and 5 patients without evidence of liver disease. Free fatty acids were found in all liver biopsies. Patients with morbid obesity or alcoholic liver disease had significantly higher fatty acid and triglyceride levels than did controls (p <, 0.01). Patients with alcoholic liver disease had significantly higher fatty acid levels than did patients with morbid obesity (p < 0.05), while there was no difference in the triglyceride concentrations between these two groups. The distribution of the fatty acids in the free fatty acid fraction differed significantly from that in the triglyceride fraction indicating a preferential incorporation of unsaturated fatty acids into triglycerides. This difference in the distribution pattern was lost in patients with the most severe forms of alcoholic liver disease. The data are consistent with the hypothesis that accumulation of free fatty acids in patients with alcoholic liver disease may be responsible for or contribute to the observed functional and morphological damages.

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Section: Discussionsupporting

confidence: 92%

“…The mean concentration of hepatic triglycerides is within the range of values observed by others in normal livers (6,15,23,24) and in steatosis associated with ALD or obesity (15,16,(24)(25)(26)(27). No data are available for comparison with the observed mono-and diglyceride levels.…”

Section: Discussionsupporting

confidence: 71%

Hepatic Free Fatty Acids in Alcoholic Liver Disease and Morbid Obesity

et al. 2007

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“…The other peaks can be considered as the biomarkers of fatty liver ( ). The molecules that correspond to the m/z values of 422, 438, 390, 567, and 613 were not reported in the past studies [6][7][8] . Hence, our data suggested that dMALDI-MS was successful in identifying a new profile of fatty liver, although the new molecules could not be identified in this study.…”

Section: Resultsmentioning

confidence: 82%

“…In order to clarify the pathogenesis of NASH, it is important to analyze the metabolites in the fatty liver. Accordingly, the metabolites in fatty liver were analyzed by conventional mass spectrometric approaches such as high-performance liquid chromatography, mass spectrometry, or gas chromatography/mass spectrometry [6][7][8] . These conventional approaches are essential for the analysis of metabolites and for determining metabolic profiles 9,10) .…”

Section: Introductionmentioning

confidence: 99%

“…This method can reveal metabolic dynamics even with a trace amount of biopsy specimen without the need for pretreatment procedures such as extraction, derivatization, or purification. dMALDI-MS is being increasingly used for the analysis of biological information1 [3][4][5][6][7][8][9][10][11][12][13][14][15] . In this study, we performed an unsupervised multivariate analysis principal component analysis (PCA) for the non-targeting screening of the starvation-induced metabolites in the fatty liver tissue section to determine whether the analysis using dMALDI-MS can help determine a new profile of fatty liver.…”

Section: Introductionmentioning

confidence: 99%

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Principal Component Analysis of Direct Matrix-Assisted Laser Desorption/Ionization Mass Spectrometric Data Related to Metabolites of Fatty Liver

2009

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Non-alcoholic steatohepatitis (NASH) is a common liver disease. NASH is characterized by fatty liver, along with inflammation. Most people with NASH are not aware of their condition, even though NASH can lead to hepatic cirrhosis. Several approaches have been tested to clarify the pathology of NASH. However, the mechanism of onset of NASH was not well-defined. In this study, a supervised multivariate analysis (principal component analysis) approach using direct matrix-assisted laser desorption/ionization mass spectrometry (dMALDI-MS) was applied to the analysis of metabolites in starvation-induced fatty liver tissue sections. This approach does not require complex pretreatments. We investigated the characteristic dynamics of metabolites in fatty liver. This approach can be applied to the analysis of human biopsy specimens of fatty liver in future studies.

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The fatty acid pattern of adipose tissue and liver triglycerides according to fat droplet size in liver parenchymal cells of diabetic subjects

et al. 1974

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111 40 diabetic inpatients the fatty acid pattern of triglycerides in liver Iat and in subcutaneous adipose tissue was determined by gas liquid chromatography. With rising size oI fat droplets in liver parenehyreal cells there was a significant increase of palmitic acid (C:16), oleic acid (C 18: 1) and linoleie acid (C 18:2) as well as a decrease of myristoleic acid (C 14 : 1), araehidonic (C 20:4) and eieosapentaenoie acid (C 20:5) in liver tri-glyeerides, resulting in a fatty acid composition in big droplets similar to that of adipose tissue. Fatty acid pattern of subcutaneous depot fat was strikingly constant.

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Gas-chromatographic Analysis of the Fatty Acid Composition of Human Fatty Liver

Cited by 14 publications

References 0 publications

Hepatic Free Fatty Acids in Alcoholic Liver Disease and Morbid Obesity

Hepatic Free Fatty Acids in Alcoholic Liver Disease and Morbid Obesity

Principal Component Analysis of Direct Matrix-Assisted Laser Desorption/Ionization Mass Spectrometric Data Related to Metabolites of Fatty Liver

The fatty acid pattern of adipose tissue and liver triglycerides according to fat droplet size in liver parenchymal cells of diabetic subjects

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