Gardenia jasminoides protects against cerulein-induced acute pancreatitis

Jung, Won Yong; Chae, Young-Seok; Kim, Doyun; Seo, Sang-Wan; Park, Hee-Je; Bae, Gi‐Sang; Kim, Tae-Hyeon; Oh, Hyo-Jeong; Yun, Ki-Jung; Park, Raekil; Kim, Jong‐Suk; Kim, Eun-Cheol; Hwang, Sung-Yeon; Park, Sung‐Joo; Song, Ho‐Joon

doi:10.3748/wjg.14.6188

Cited by 38 publications

(21 citation statements)

References 23 publications

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“…Gardeniae Fructus (GF) is a traditional Chinese medicine, which has alleviating effect on cytotoxicity [20], antioxidant activity [21] and protective action on pancreatitis [22]. We have also reported [23,24] that GF composed in Bofutsushosan alleviates the abnormal glucose/lipid metabolism in STZ-diabetic mice.…”

Section: Q Yu Et Al 158mentioning

confidence: 87%

Anti-Hyperglycemic Effect of Single Administered Gardeniae Fructus in Streptozotocin-Induced Diabetic Mice by Improving Insulin Resistance and Enhancing Glucose Uptake in Skeletal Muscle

Yu¹,

Takahashi²,

Nomura³

et al. 2013

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The mechanisms of Gardeniae Fructus (GF) for anti-hyperglycemic action were demonstrated in streptozotocin (STZ)-diabetic mice. Six hours after single intraperitoneal administration of GF (300 mg/kg) or H 2 O into 3 hour-fasted STZ-diabetic mice, glucose and insulin tolerances were assessed by intraperitoneal glucose (1.5 g/kg) tolerance test (IPGTT) and intraperitoneal insulin (0.65 U/kg) tolerance test (IPITT), respectively. Effects of GF on insulin signaling pathways in soleus muscle such as glucose uptake, expression of glucose transporter 4 (GLUT4) in the plasma membrane and phosphorylation of Akt (P-Akt) in cytosolic fraction were examined in STZ-diabetic mice. In IPGTT test, GF significantly accelerated clearance of exogenous glucose and its glucose-lowering action was greater than H 2 O-treated control in STZ-diabetic mice. GF also promoted an exogenous glucose-increased insulin level in STZ-diabetic mice. In IPITT test, GF decreased glucose level to the greater extent than H 2 O-treated control in STZ-diabetic mice. Furthermore, GF significantly decreased high HOMA-IR in STZ-diabetic mice from 21.6 ± 2.4 to 12.4 ± 1.9 (mg/dl × μU/ml). These results implied that GF improved insulin resistance in STZ-diabetic mice. GF increased glucose uptake of soleus muscle 1.5 times greater than H 2 O-treated control in STZ-diabetic mice. GF enlarged insulin (10 nmol/ml)-increased glucose uptake to 1.8 time-greater. Correspondingly, GF increased expression of GLUT4 in the plasma membrane of soleus muscle to 1.4 time-greater, and P-Akt in the cytosolic fraction of soleus muscle to 1.9 time-greater than those in H 2 Otreated control. In conclusion, the improvement of GF on insulin resistance is associated with the repair of insulin signaling via P-Akt, GLUT4 and glucose uptake pathway in soleus muscle of STZ-diabetic mice.

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Section: Q Yu Et Al 158mentioning

confidence: 87%

Anti-Hyperglycemic Effect of Single Administered Gardeniae Fructus in Streptozotocin-Induced Diabetic Mice by Improving Insulin Resistance and Enhancing Glucose Uptake in Skeletal Muscle

Yu¹,

Takahashi²,

Nomura³

et al. 2013

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“…AP was induced by a supra-maximal concentration of cerulein (50 μg/kg), a stable cholecystokinin (CCK) analogue, by administering it intraperitoneally every hour for a total of 6 h, as described previously (13). The mice were fed orally with CL (0.05, 0.1, 0.5 and 1 g/kg, n=6 each) or normal saline (control group, n=6) for 7 days, followed by the intraperitoneal injection of cerulein (50 μg/kg) or saline every hour for a total of 6 h. The mice were sacrificed 6 h after the completion of the cerulein injections.…”

Section: Methodsmentioning

confidence: 99%

“…From each treatment group of 6 mice, the entire pancreas was examined and semi-quantified based on the degree of necrosis, vacuolization, inflammation and edema. Using a previously described method (13), entire sections (a minimum of 100 fields) of pancreas were examined from each sample and scored on a scale of 0-3 (0 being normal and 3 being severe), based on the number of necrotic acinar cells, and the presence of vacuolization, interstitial edema and inflammation. The characteristics included were: The presence of acinar-cell ghosts, vacuolization and swelling of the acinar cells, and/or the destruction of the histoarchitecture of whole or parts of the acini.…”

Section: Methodsmentioning

confidence: 99%

“…Neutrophil sequestration in the pancreas was quantified by measuring the tissue MPO activity (13). Tissue samples were thawed, homogenized in 20 mmol/l phosphate buffer (pH 7.4), centrifuged (10,000 x g, 10 min, 4˚C), and the resulting pellet was resuspended in 50 mmol/l phosphate buffer (pH 6.0) containing 0.5% hexadecyltrimethylammonium bromide (Sigma).…”

Section: Measurement Of Serum Amylase and Lipase Levelsmentioning

confidence: 99%

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Protective effects of Curcuma longa against cerulein-induced acute pancreatitis and pancreatitis-associated lung injury

Seo¹,

Bae²,

Kim³

et al. 2010

Int J Mol Med

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Abstract. Curcuma longa (CL) has been reported to possess a variety of pharmacological activities. However, the effects of CL on acute pancreatitis (AP) have not yet been determined. To this end, we examined the effects of CL on ceruleininduced AP. Cell viability and cytokine productions were measured in pancreatic acini. Mice were divided into 3 groups: i) Normal group, ii) normal saline-treated group, iii) group treated with CL at a dose of 0.05, 0.1, 0.5 and 1 g/kg. CL was administered orally to mice for 7 days. The mice were intraperitoneally injected with the stable cholecystokinin analogue, cerulein (50 μg/kg), every hour for a total of 6 h. The mice were sacrificed 6 h after the completion of the cerulein injections. Blood samples were obtained to determine serum amylase, lipase and cytokine levels. The pancreas was rapidly removed for morphological examination, measurement of tissue myeloperoxidase activity, as well as the level of cytokines and heme oxygenase-1 (HO-1). The CL treatment reduced cerulein-induced cell death and cytokine production in pancreatic acini. The administration of CL significantly ameliorated the severity of pancreatitis and pancreatitisassociated lung injury, as was shown by the reduction in pancreatic edema, neutrophil infiltration, vacuolization, necrosis, serum amylase, lipase and cytokine levels, and mRNA expression of multiple inflammatory mediators such as interleukin (IL)-1ß and -6 and tumor necrosis factor (TNF)-·. In order to identify the regulatory mechanism of CL on cerulein-induced pancreatitis, we examined the level of HO-1 in the pancreas. We found that the administration of CL induced HO-1. Our results suggest that CL plays a protective role in the development of AP and pancreatitisassociated lung injury.

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“…38 The main mechanism of hypoglycemic effect of geniposide was mediated by inhibiting the GP and G6Pase activities. 39 The fibril precursors of islet amyloid polypeptide (IAPP) are cytotoxic to pancreatic β cells which lead to β-cell dysfunction in type 2 diabetes mellitus (T2DM).…”

Section: Anti-diabetic and Anti-atherosclerotic Activitiesmentioning

confidence: 99%

Phytochemistry, Pharmacological Activities and Intellectual Property Landscape of Gardenia jasminoides Ellis: a Review

Phatak¹

2015

Phcog J

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Gardenia jasminoides protects against cerulein-induced acute pancreatitis

Abstract: These results suggest that GJ attenuated the severity of AP as well as pancreatitis-associated lung injury.

Cited by 38 publications

References 23 publications

Anti-Hyperglycemic Effect of Single Administered Gardeniae Fructus in Streptozotocin-Induced Diabetic Mice by Improving Insulin Resistance and Enhancing Glucose Uptake in Skeletal Muscle

Anti-Hyperglycemic Effect of Single Administered Gardeniae Fructus in Streptozotocin-Induced Diabetic Mice by Improving Insulin Resistance and Enhancing Glucose Uptake in Skeletal Muscle

Protective effects of Curcuma longa against cerulein-induced acute pancreatitis and pancreatitis-associated lung injury

Phytochemistry, Pharmacological Activities and Intellectual Property Landscape of Gardenia jasminoides Ellis: a Review

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