Gap junction formation in myometrium: control by estrogens, progesterone, and prostaglandins

Garfield, Robert E.; Kannan, Mathur; Daniel, E. E.

doi:10.1152/ajpcell.1980.238.3.c81

Cited by 318 publications

(100 citation statements)

References 14 publications

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“…In the rat myometrium, generation of gap junctions was inhibited by indomethacin and increased by PGH2 and arachidonic acid (Garfield et al, 1980). The reduction in the distribution of Lucifer Yellow induced by diclofenac observed in the present study is consistent with the findings of the aforementioned studies on smooth muscle.…”

Section: Discussionsupporting

confidence: 92%

Evidence for the involvement of the cyclooxygenase-metabolic pathway in diclofenac-induced inhibition of spontaneous contraction of rat portal vein smooth muscle cells

Shimamura

Kimura

Zhou

et al. 2005

J. Smooth Muscle Res.

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The effects of diclofenac, a cyclooxygenase (COX) inhibitor, were investigated on spontaneous phasic contractions of longitudinal preparations of the rat portal vein. Diclofenac produced a concentration-dependent decrease in the amplitude of these spontaneous phasic contractions. Diclofenac (30 µM) decreased the amplitude of the spontaneous phasic increase in the F340/F380 ratio of Fura PE3, an indicator of intracellular Ca 2+ concentration. It also reduced the number of action potentials in each burst discharge without changing the resting membrane potential of longitudinal smooth muscle cells. The extent of the distribution of Lucifer Yellow injected into a smooth muscle cell was decreased in the presence of diclofenac (30 µM). Both AH6809, a prostanoid EP receptor antagonist, and SQ22536, an adenylate cyclase inhibitor, decreased the amplitude of the spontaneous contractions. On the other hand, neither ozagrel, a thromboxane synthase inhibitor, nor SQ29548, a prostanoid TP receptor antagonist, significantly affected spontaneous contractions. These results indicate that diclofenac inhibits the amplitude of spontaneous contractions of the rat portal vein through inhibition of electrical activity, which may be related to an inhibition of the cyclooxygenase pathway.

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Section: Discussionsupporting

confidence: 92%

Evidence for the involvement of the cyclooxygenase-metabolic pathway in diclofenac-induced inhibition of spontaneous contraction of rat portal vein smooth muscle cells

Shimamura

Kimura

Zhou

et al. 2005

J. Smooth Muscle Res.

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“…Oestrogen becomes the dominant hormone at parturition in the ewe, when it induces cervical ripening (Ellwood 1980, Winn et al 1994, Cheah et al 1995, Silva et al 1995 and the formation of myometrial gap junctions (Garfield et al 1980, Ciray et al 1996. Although the Figure 4 The expression of oestradiol receptor (E2R) mRNA in the maternal caruncles or villi was localised by in situ hybridisation.…”

Section: Discussionmentioning

confidence: 99%

Regulation of oxytocin receptor in the placentome capsule throughout pregnancy in the ewe: the possible role of oestradiol receptor, progesterone receptor and aromatase

Leung

Reynolds²,

Wathes³

1998

Journal of Endocrinology

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The hormonal regulation of uterine oxytocin receptors (OTR) during the establishment of pregnancy and at parturition has been studied extensively, but little information is available during mid-pregnancy. This study investigated the localisation of OTR mRNA in the ovine placentome throughout gestation and related this to expression patterns for the putative regulatory agents aromatase, oestradiol receptor, progesterone receptor and oxytocin. Placentomes were collected at regular intervals throughout pregnancy for in situ hybridisation analysis and immunocytochemistry (oestradiol receptor only). Results were quantified by optical density measurements of autoradiographs. Progesterone receptor mRNA was localised to the caruncular tissues on day 30 but became undetectable by day 34. Aromatase mRNA appeared in the fetal villi at days 34-40, with concentrations peaking at days 52-55 and again at days 132-137. Oestradiol receptor mRNA was localised to the caruncular tissues from days 13 to 30 and found in the maternal villi and placentome capsule from days 45 to 70. Oestradiol receptor protein was barely detectable in either tissue. OTR mRNA was localised to the placentome capsule at days 34-40, remaining high at day 45 and declining to basal levels by days 132-137. Oxytocin mRNA was not detected in the placentome. In conclusion: (1) progesterone acting via its receptor may suppress the expression of aromatase and OTR in early pregnancy; (2) the up-regulation of OTR expression in the capsule may not involve the oestradiol receptor; (3) there is a differential regulation between different regions of the uterus as the increase in the placentome capsule occurs at a time when concentrations in the rest of the endometrium and myometrium remain low; (4) oestradiol receptor expression in the placentome may be regulated at the translational level; and (5) there is no local production of oxytocin in the sheep placenta. The role of OTRs in the capsule during mid-pregnancy remains to be determined.

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“…11 Progesterone is essential for maintenance of pregnancy and helps in prolongation of pregnancy. [12][13][14][15][16][17] Delaying delivery may reduce the rate of long term morbidity by facilitating maturity of vital organs, help in optimum action of the administered glucocorticoids, helps in transfer to higher centre with NICU facilities. Though exact mechanism of action until very recently.…”

Section: Introductionmentioning

confidence: 99%

“…Though exact mechanism of action until very recently. [12][13][14][15][16][17][18] suggested mechanisms were -1. It acts primarily through establishing uterine quiescence and maintains cervical length.…”

Section: Introductionmentioning

confidence: 99%

Comparative Study to Assess the Efficacy Between Intramuscular and Vaginal Micronized Progesterone to Prevent Threatened Premature Labour

Patra¹,

Chattopadhyay²,

Munsi³

et al. 2015

jemds

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Preterm birth remains a major clinical problem. Prematurity is not only a major cause of perinatal mortality but also leads to greater risk for short and long term complications including disability and impediments in growth and mental development. To compare vaginal with intramuscular progesterone administration to prevent preterm labor and to detect the effect of both on the uterine and foetal circulations. STUDY DESIGN: comparative interventional study. POPULATION: 100 pregnant women attending NRSMCH Kolkata, Obstetrics emergency with threatened preterm labour before 34 weeks of gestation. STUDY PERIOD: one year. METHODS: Gestational age was confirmed clinically and by USG of early weeks of gestation. Efficacy and tolerability of progesterone in two routes i.e., intramuscular and vaginal for prevention of threatened preterm labour were compared. OUTCOME: Ante partum, intrapartum and perinatal outcomes were compared between two groups by statistical analysis of data's using chi-square test and student-t test. CONCLUSION: Vaginal progesterone was as effective as intramuscular progesterone in reducing preterm birth with fewer side effects in favor of vaginal route.

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Gap junction formation in myometrium: control by estrogens, progesterone, and prostaglandins

Cited by 318 publications

References 14 publications

Evidence for the involvement of the cyclooxygenase-metabolic pathway in diclofenac-induced inhibition of spontaneous contraction of rat portal vein smooth muscle cells

Evidence for the involvement of the cyclooxygenase-metabolic pathway in diclofenac-induced inhibition of spontaneous contraction of rat portal vein smooth muscle cells

Regulation of oxytocin receptor in the placentome capsule throughout pregnancy in the ewe: the possible role of oestradiol receptor, progesterone receptor and aromatase

Comparative Study to Assess the Efficacy Between Intramuscular and Vaginal Micronized Progesterone to Prevent Threatened Premature Labour

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