Ganoderic Acid A Targeting β-Catenin in Wnt Signaling Pathway: In Silico and In Vitro Study

Gill, Balraj Singh; Kumar, Sanjeev; Navgeet,

doi:10.1007/s12539-016-0182-7

Cited by 18 publications

(15 citation statements)

References 26 publications

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“…Although lactate dehydrogenase can be inhibited by galloflavin without affecting the energetic balance of normal tissues, 37 ROS production, and expression of lactose‐synthesizing genes are not altered. As described in previous studies, ganoderic acid is a substrate with antioxidant activities, intracellular ROS scavenging, inhibition of the glucose metabolic shift (including glucose uptake, lactate production, pyruvate, and acetyl‐coenzyme production), insulin sensitivity accelerating, and amelioration of glucose homeostasis and inhibition of NF‐κB 33‐36 . In our study, treatment of cells with ganoderic acid A under the high dose of glucose reduced fermentative glycolysis and ROS production but greater lactose concentration and expression of lactose synthesis genes, indicating its role in benefiting for lactation of MECs under the high glucose dose.…”

Section: Discussionsupporting

confidence: 80%

“…Different materials were also added to the culture medium with the high dose of glucose for another 24 hours to investigate their ability to regulate glucose metabolism or NF‐κB2. Ganoderic acid A (Cat#81907622, Derick, Chengdu, China, treated concentration: 0.25 μM) was used for its function of antioxidative activities, intracellular reactive oxygen species (ROS) scavenging, inhibitory effect on NF‐κB, and inhibition of the glucose metabolic shift 33‐36 . Galloflavin (Cat#568‐80‐9, Aladdin, Los Angeles, USA, treated concentration: 100 μM) was used for the inhibition of overactivated fermentative glycolysis 37 .…”

Section: Methodsmentioning

confidence: 99%

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Excessive supply of glucose elicits an NF‐κB2‐dependent glycolysis in lactating goat mammary glands

et al. 2020

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During lactation, an improper glucose supply often threatens mammary gland (MG) health. However, information is limited on the metabolic trajectories and molecules that regulate lactating MGs with an excessive glucose supply. Based on the network analysis of transcriptome and microRNAs, we found that the oversupply of glucose‐induced severe glucose metabolic disorders in MGs of lactating goats, shifting lactose synthesis to acute fermentative glycolysis which caused increased flux of glucose metabolism into lactate. Moreover, NF‐κB2 played a key role in regulating glycolysis, exhibiting a metabolic shift when MGs had an excessive supply of glucose. In primary mammary epithelial cells, fermentative glycolysis, and intracellular concentration of reactive oxygen species (ROS) were reduced by ganoderic acid A through blocking NF‐κB2, while activation of NF‐κB2 with phorbol myristate acetate (PMA) upregulated fermentative glycolysis and increased cellular ROS accumulation under excessive glucose. Thus, we established an NF‐κB2‐targeting method to reform the metabolic shift toward glycolysis caused by glucose oversupply by integrating NF‐κB2 blockade and intracellular ROS scavenging.

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Section: Discussionsupporting

confidence: 80%

Section: Methodsmentioning

confidence: 99%

Excessive supply of glucose elicits an NF‐κB2‐dependent glycolysis in lactating goat mammary glands

et al. 2020

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“…lucidum that support its antioxidant and protective effects. A pancreatic cell study suggested β -catenin in the Wnt signaling pathway as a target of ganoderic acid A, thus leading to cell protection and effective scavenging of ROS [ 54 ]. The Wnt signaling pathways transfer the signals from extracellular to intercellular and are stimulated by the Wnt protein binding to the cytoplasmic family receptor, which occurs in downstream cell signaling and controls the transcription of genes.…”

Section: Resultsmentioning

confidence: 99%

“…mTOR is the main target of G. lucidum, and this finding supports its antioxidant and cardioprotective effects. G. lucidum inhibits the Wnt pathway [54] and may decrease the activity of mTOR via the Wnt/GSk/mTOR signal pathway. A pathologically stressed heart reactivates the Wnt signal pathway, which is modulated during left ventricular remodeling [77].…”

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confidence: 99%

Associated Targets of the Antioxidant Cardioprotection of Ganoderma lucidum in Diabetic Cardiomyopathy by Using Open Targets Platform: A Systematic Review

Shaher

Qiu

Wang

et al. 2020

BioMed Research International

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Even with substantial advances in cardiovascular therapy, the morbidity and mortality rates of diabetic cardiomyopathy (DCM) continually increase. Hence, a feasible therapeutic approach is urgently needed. Objectives. This work is aimed at systemically reviewing literature and addressing cell targets in DCM through the possible cardioprotection of G. lucidum through its antioxidant effects by using the Open Targets Platform (OTP) website. Methods. The OTP website version of 19.11 was accessed in December 2019 to identify the studies in DCM involving G. lucidum. Results. Among the 157 cell targets associated with DCM, the mammalian target of rapamycin (mTOR) was shared by all evidence, drug, and text mining data with 0.08 score association. mTOR also had the highest score association 0.1 with autophagy in DCM. Among the 1731 studies of indexed PubMed articles on G. lucidum published between 1985 and 2019, 33 addressed the antioxidant effects of G. lucidum and its molecular signal pathways involving oxidative stress and therefore were included in the current work. Conclusion. mTOR is one of the targets by DCM and can be inhibited by the antioxidative properties of G. lucidum directly via scavenging radicals and indirectly via modulating mTOR signal pathways such as Wnt signaling pathway, Erk1/2 signaling, and NF-κB pathways.

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“…Data from Wang et al have reported that GA‐A impairs proliferation and invasion, and promotes apoptosis in human hepatocellular carcinoma cells. Gill et al have suggested that GA‐A inhibits properties of proliferation and viability, and intracellular ROS of pancreatic cancer cells. Significantly, GA‐A heightens chemosensitivity of hepatocellular carcinoma cells to Cisplatin, raising its possibility for being utilized as a perspective combined chemotherapeutic agents for cancer treatment .…”

Section: Discussionmentioning

confidence: 99%

Ganoderic acid A holds promising cytotoxicity on human glioblastoma mediated by incurring apoptosis and autophagy and inactivating PI3K/AKT signaling pathway

Cheng

Xie

2019

J Biochem & Molecular Tox

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Ganoderic acid A (GA-A), recognized as a lanostanetriterpene isolated fromGanoderma lucidum, demonstrates an efficient antitumor activity in multiple cancers.To date, it is unclear whether and how GA-A functions on human glioblastoma (GBM).To unravel the functional significance of GA-A on human glioblastoma (GBM), the cell-counting kit-8 and transwell assays were used to detect proliferation, migration, and invasion of human GBM cell after GA-A treatment. Then, we utilized the flow cytometry and western blot to further evaluate the effect of GA-A on GBM cell.Further, activities of autophagy and PI3K/AKT signaling were assessed by Western blot assay. We found that GA-A significantly inhibited proliferation, migration, and invasion of GBM cell. Additionally, GA-A markedly triggered cell apoptosis, which incarnated an elevation trend in apoptotic percentage, simultaneously, an increased level of proapoptosis protein (Bax and active caspase-3) and a decreased level of antiapoptosis protein (Bcl-2), induced by GA-A treatment. Meanwhile, levels of two well-known autophagy markers (beclin 1 and LC3 II) increased while another autophagic substrate (P-62) was reduced. Moreover, the expressions levels of phosphorylated AKT, mTOR, p-P70S6K, and cyclin D1 in the PI3K/AKT pathway were significantly reduced, which revealed GA-A repressed the activation of PI3K/AKT signaling pathway. Collectively, these results indicate that GA-A may encourage U251 cell growth and invasion/migration inhibition, apoptosis, and autophagy through the inactivation of PI3K/AKT signaling pathway in human GBM. Hence, GA-A may be a potent antitumorigenic agent for human GBM in future clinical practice.

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Ganoderic Acid A Targeting β-Catenin in Wnt Signaling Pathway: In Silico and In Vitro Study

Cited by 18 publications

References 26 publications

Excessive supply of glucose elicits an NF‐κB2‐dependent glycolysis in lactating goat mammary glands

Excessive supply of glucose elicits an NF‐κB2‐dependent glycolysis in lactating goat mammary glands

Associated Targets of the Antioxidant Cardioprotection of Ganoderma lucidum in Diabetic Cardiomyopathy by Using Open Targets Platform: A Systematic Review

Ganoderic acid A holds promising cytotoxicity on human glioblastoma mediated by incurring apoptosis and autophagy and inactivating PI3K/AKT signaling pathway

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