Gangrenous necrosis of the diabetic foot caused by<i>Fusarium acutatum</i>

Taj-Aldeen, Saad J.; Gené, Josepa; Al‐Bozom, Issam; Buzina, Walter; Cano, J.; Guarro, Josep

doi:10.1080/13693780500543246

Cited by 27 publications

(14 citation statements)

References 30 publications

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“…The spectrum of microbial infection occurring in these diabetic foot infections has already been reported. While most of these studies have focused only on the bacteria, very few studies have reported the presence of filamentous fungi [5,6]. Sometimes, the presence of low pathogenic yeast has also been reported in some studies [2,7].…”

Section: Introductionmentioning

confidence: 96%

Prevalence of yeast in diabetic foot infections

Sugandhi

Prasanth

2016

Int J Diabetes Dev Ctries

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Diabetic foot infections are a serious threat which cause a long term of hospitalization of diabetes patients and lead to amputations. Hence, prompt diagnosis is an important criterion in the treatment of these infections. This study was carried out to determine the prevalence of yeast in diabetic foot infections. The pus samples were collected from patients presenting with diabetic foot ulcer and processed for microbiological investigation. The patients investigated comprised of 82 males and 78 females. Most of the patients were grade I ulcers followed by grade II ulcers. Out of 160 samples, 138 samples were culture positive, in which yeast isolate Candida glabrata was the predominant isolate followed by Candida albicans, Candida dubliniensis, Candida krusei and Candida tropicalis. C. albicans and C. dubliniensis were differentiated by using tobacco agar due to the presence of hyphal fringes. The methylene blue sabouraud dextrose agar and corn meal agar were also used to differentiate C. albicans from non albicans. The antifungal susceptibility pattern showed 29 % of C. albicans was sensitive to Amphotericin B and Clotrimazole, while 75 % of C. glabrata was sensitive to Amphotericin B, Ketoconazole and Itraconazole. C. dublinensis and C. albicans showed the highest percentage of resistant to Ketoconazole, Fluconazole, Nystatin and Itraconazole. Thus, the results indicating that effective alternate drug of choice are required and the proper selection of antifungal agents will play an important role for the treatment of fungal foot infections. This study will also add substantial knowledge of yeast as one of the pathogenic organisms in diabetic foot infections.

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Section: Introductionmentioning

confidence: 96%

Prevalence of yeast in diabetic foot infections

Sugandhi

Prasanth

2016

Int J Diabetes Dev Ctries

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“…White piedra is a clinical condition caused by Trichosporon species (1,2). The most distinctive feature of this mycosis is the production of white-brown nodules adhered to the hair shaft that may easily be detached, in contrast with black piedra nodules (3). Despite awareness of white piedra in the medical field since the 19th century (4), there is little knowledge about its mechanisms of infection in humans (5,6).…”

Section: Introductionmentioning

confidence: 99%

Experimental white piedra: a robust approach to ultrastructural analysis, scanning electron microscopy and etiological discoveries

Inácio

Rocha

Barbosa

et al. 2015

Experimental Dermatology

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White piedra is a fungal infection characterized by nodules comprised of Trichosporon species and restricted to the extrafollicular portion of the hair shaft. The diagnosis is based on clinical and mycological characteristics, and must be confirmed with a precise identification of the etiological agent. This research aimed to develop an in vitro infection model of white piedra and analyze its morphological and ultra-structural aspects. In the process, hair infection was induced using eight isolates of the genus Trichosporon maintained in the Culture Collection Micoteca URM. The ITS and IGS1 regions were sequenced for taxonomic confirmation. Scanning Electron Microscope (SEM) was performed at the Strategic Center for Northeast Technologies (CETENE). The scanning electron microscope was equipped with an Energy Dispersion Spectrometer (EDS). The Trichosporon isolates were identified as Trichosporon asahii (6) and Trichosporon montevideense (2) by internal transcript spacer (ITS) region and intergenic spacer 1 region (IGS1) sequencing. All eight strains were used to induce the in vitro hair infection, and nodules formed after the incubation period. Temperature variations and high humidity were not observed to be related to the development of this hair disease. The main chemical constituents detected in the nodules were carbon, nitrogen and oxygen, as well as a low level of sulfur. The absence of calcium, combined with the low level of sulfur, might explain the soft nature of the white piedra nodules. This study demonstrated that several Trichosporon species may be responsible for causing white piedra.

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“…Based on an extensive literature review, Nucci and Anaissie (48) recently recorded 12 morphospecies associated with fusarial infections within the immunocompromised patient population. However, phylogenetic species recognition based on genealogical concordance of multilocus DNA sequence data (herein referred to as GCPSR) (79) has identified at least 69 clinically important Fusarium species (Table 1) (49,54,56,57,70,85). Phylogenetic species in these studies were recognized if they received Ն70% maximum parsimony (MP) bootstrap support (78) from the majority of the individual gene partitions and the combined data set and if their monophyly was not contradicted by analyses of any of the individual singlegene partitions.…”

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confidence: 99%

Internet-Accessible DNA Sequence Database for Identifying Fusaria from Human and Animal Infections

O’Donnell¹,

et al. 2010

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Because less than one-third of clinically relevant fusaria can be accurately identified to species level using phenotypic data (i.e., morphological species recognition), we constructed a three-locus DNA sequence database to facilitate molecular identification of the 69 Fusarium species associated with human or animal mycoses encountered in clinical microbiology laboratories. The database comprises partial sequences from three nuclear genes: translation elongation factor 1␣ (EF-1␣), the largest subunit of RNA polymerase (RPB1), and the second largest subunit of RNA polymerase (RPB2). These three gene fragments can be amplified by PCR and sequenced using primers that are conserved across the phylogenetic breadth of Fusarium. Phylogenetic analyses of the combined data set reveal that, with the exception of two monotypic lineages, all clinically relevant fusaria are nested in one of eight variously sized and strongly supported species complexes. The monophyletic lineages have been named informally to facilitate communication of an isolate's clade membership and genetic diversity. To identify isolates to the species included within the database, partial DNA sequence data from one or more of the three genes can be used as a BLAST query against the database which is Web accessible at FUSARIUM-ID (http://isolate.fusariumdb.org) and the Centraalbureau voor Schimmelcultures (CBS-KNAW) Fungal Biodiversity Center (http://www.cbs.knaw.nl/fusarium). Alternatively, isolates can be identified via phylogenetic analysis by adding sequences of unknowns to the DNA sequence alignment, which can be downloaded from the two aforementioned websites. The utility of this database should increase significantly as members of the clinical microbiology community deposit in internationally accessible culture collections (e.g., CBS-KNAW or the Fusarium Research Center) cultures of novel mycosis-associated fusaria, along with associated, corrected sequence chromatograms and data, so that the sequence results can be verified and isolates are made available for future study.

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Gangrenous necrosis of the diabetic foot caused byFusarium acutatum

Cited by 27 publications

References 30 publications

Prevalence of yeast in diabetic foot infections

Prevalence of yeast in diabetic foot infections

Experimental white piedra: a robust approach to ultrastructural analysis, scanning electron microscopy and etiological discoveries

Internet-Accessible DNA Sequence Database for Identifying Fusaria from Human and Animal Infections

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