Gamma Tocopherol and Lovastatin Additively Induced Apoptosis in Human Colorectal Carcinoma Cell Line (HT29)

Zeidooni, Leila; Rezaeian, Mohsen; Tabar, Mahmoud Hashemi

doi:10.17795/jjnpp-4860

Cited by 2 publications

(2 citation statements)

References 23 publications

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“…This effect is induced by decreasing the expression of the ABCB1 and p-glycoprotein genes, inducing apoptosis through increasing the P53 expression, increasing the Bax/Bcl-2 expression ratio, and increasing CDKN1A, ultimately leading to the enhancement of cell cycle arrest in the G2/M phase along with paclitaxel (30). Besides, Bcl-2 is the first known gene associated with apoptosis, which plays a role in tumor formation (31). The members of the Bcl-2 family in mammals can control the internal pathway of apoptosis by inhibiting the release of cytochrome C and other mitochondrial membrane proteins into cytosol.…”

Section: Discussionmentioning

confidence: 99%

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Cytotoxic and Apoptotic Effects of Ferulic Acid on Renal Carcinoma Cell Line (ACHN)

Karimvand

Kalantar

Khodayar

2020

Jundishapur J Nat Pharm Prod

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Background: Polyphenolic compounds have anti-proliferative effects and can trigger apoptosis in cancer cells. Ferulic acid is excessively found in various herbal products and fruits. Ferulic acid plays a role in the treatment of neurodegenerative diseases, cancer, diabetes, cardiovascular disease, inflammation, and bacterial and viral infections. Objectives: The purpose of this study was to evaluate the anticancer effect of ferulic acid on renal carcinoma cells (ACHN). Methods: To assess the anti-proliferative effect of ferulic acid, the renal carcinoma cell line (ACHN) was treated with different ferulic acid concentrations (10, 20, 40, 80, and 160 μM) for 24, 48, and 72 hours. Cell viability was measured using the MTT assay. The apoptosis of cancer cells was evaluated by flow cytometry and real-time PCR. Results: The IC50 of ferulic acid against ACHN cells was determined to be 30 μM at 72 hours with the MTT assay. The treatment of cells with ferulic acid concentrations of 30 and 60 μM caused a significant increase in the apoptosis index. The Bcl-2 gene expression level was significantly lower in the treated group than in the control group, and the Bax gene expression level was significantly higher in the treated group than in the control group (P < 0.001). Conclusions: The results of this study showed the apoptotic activity of ferulic acid against ACHN cells. These results can be helpful in the better understanding of the anticancer mechanism of ferulic acid, suggesting this substance as an alternative drug or in combination with conventional chemical treatments for cancer treatment.

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Section: Discussionmentioning

confidence: 99%

“…Indeed, these two groups restrain each other's actions. The balance between their activities usually determines the presence or absence of cell apoptotic induction (31).…”

Section: Discussionmentioning

confidence: 99%