Gamma-Ray Radiation and Sodium Azide (NaN3) Mutagenic Efficiency in Rice

Ando, Akihiko; Montalván, Ricardo

doi:10.13082/1984-7033.v01n04a02

Cited by 11 publications

(8 citation statements)

References 8 publications

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“…Classes of chlorophyll mutants observed in CO 51 were albino and xantha. Similar, spectrum of chlorophyll mutants induced by Sodium Azide were reported by Ando et al, (2001) and Nilan et al, (1967).…”

Section: Resultssupporting

confidence: 86%

Mutagenic effectiveness and efficiency of sodium azide in rice varieties

Vinithashri

Manonmani²,

Anand³

et al. 2020

EJPB

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This study was taken up to assess the chlorophyll spectrum induced by Sodium Azide mutagen in two rice cultivars-BPT 2231 and CO 51. The mutagenic effectiveness and efficiency of Sodium Azide across the two genotypes were also observed. The chlorophyll spectrum observed in M 2 generation of BPT 2231 is consisted of albino, xantha, chlorina, striata and xantha viridis. In CO 51, albino and xantha occupied the entire chlorophyll spectrum. Albino was predominant in both the varieties with 1.2 per cent in BPT 2231 and 1.15 per cent in CO 51 at 1mM concentration. The mutagenic frequency was higher in BPT 2231 (7.8) than CO 51 (4.0). Considering the efficiency of a mutagen based on lethality, seedling injury and pollen sterility, BPT 2231 recorded higher values 2.79, 1.10 and 0.26 respectively. Like mutagenic frequency, the mutation rate of Sodium Azide was higher in BPT 2231(0.90) than CO 51 (0.40).

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Section: Resultssupporting

confidence: 86%

Mutagenic effectiveness and efficiency of sodium azide in rice varieties

Vinithashri

Manonmani²,

Anand³

et al. 2020

EJPB

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“…Presoaked seeds (12 h, in water) were treated with freshly prepared 0.5, 1 and 5 mM of sodium azide solution for 8 h at 25 ± 2°C. Immediately after the treatment, the seeds were washed thoroughly in running water to reduce the residual effect of the mutagen on the seed coat and were dried for next steps (Ando and Montalvan, 2001). 3.…”

Section: Mutagenesis Experimentsmentioning

confidence: 99%

“…mutagens. Despite the advantages and limitations of this method, it has been applied for improving numerous improved cultivars, in different crops (Ando and Montalvan, 2001).…”

Section: Introductionmentioning

confidence: 99%

The effects of chemical and physical mutagens on morphological and cytological characters of barley (Iranian cv. Nosrat)

G.R¹

2012

Afr. J. Biotechnol.

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Induced mutations are highly effective in enhancing natural genetic resources and have been used in developing beneficial variations for practical plant breeding. In the current study, dry grains of barley (Hordeum vulgaris L., cv. Nosrat, 2n = 2x = 14) were exposed to physical and chemical mutagens. The results of Duncan multiple rang test showed that treatments of gamma ray (control (0), 200 and 320 Gy) had highest germination percentage and formed class a. The lowest germination percentage belonged to ethyl methane sulfanate (0.7%). Mean comparison of radical length trait showed that the highest radical length belonged to gamma ray control, 200 Gy and sodium azide (SA) 0.5 mM and treatments of gamma ray 700 Gy, 1200 Gy and ethylmethane sulphonate (EMS) 0.7% had lowest radical length. Mean comparison of plumule length trait showed that treatment of SA 1 mM had highest plumule length. Cytogenetic studies have shown chromosome breakage in some induced barley seed by high dose of 700 and 1200 Gy. On the basis on growth traits analysis, sodium azide 0.5, 1 and 5 mM, ethyl methane sulfanate 0.1% and gamma ray 200 and 320 Gy are appropriate mutagens. However, it needs more consideration on mutagen, dose of mutagens and trait to have best breeding program by mutagenesis.

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“…The qualitative deviation is indicated by the albino seedling at 5 mM ( Figure 2). NaN 3 is actually reported effective to make rice seedling albino (Ando and Montalvan, 2001). The albino condition is caused by the mistakes in the chloroplast genome replication, so it makes chlorophyll abnormal (Lin et al, 2008).…”

Section: Resultsmentioning

confidence: 99%

MUTAGENIC EFFECTS OF SODIUM AZIDE ON THE GERMINATION IN RICE (Oryza sativa L. cv. INPAGO UNSOED 1)

Herwibawa

Kusmiyati

2017

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The available natural diversity highly limits the effort to improve the production of upland-rice with delicious and aromatic flavors. However, the mutagenesis can help improving the natural diversity. The aromatic-upland rice seeds, cv. Inpago Unsoed 1, were soaked in solution of NaN3 with the doses of 0 mM, 1 mM, 2 mM, 3 mM, 4 mM, 5 mM, 6 mM, 7 mM, 8 mM, 9 mM, and 10 mM, in four replications in completely randomized design. The observation included LD50, germination percentage at first-count and final-count. Data were tabulated and analyzed with CurveExpert 1.4 software for LD50, and generalized linear model in the PROC-GLM procedure of SAS 9.1 software. The means were generated and compared through Dunnett option, at probability level of 5%, in order to determine the difference in means between mutated and non-mutated seeds. The results show that the attributes of rice physiology affected by mutagenesis are sensitivity of rice to NaN3 with LD50 at 8.84 mM, and significant effect of NaN3 on the decreasing capacity of seed germination at > 6 mM for first count, and > 7 mM for final count.Keywords: germination, inpago unsoed 1, median lethal dose, rice, sodium azide

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Gamma-Ray Radiation and Sodium Azide (NaN3) Mutagenic Efficiency in Rice

Cited by 11 publications

References 8 publications

Mutagenic effectiveness and efficiency of sodium azide in rice varieties

Mutagenic effectiveness and efficiency of sodium azide in rice varieties

The effects of chemical and physical mutagens on morphological and cytological characters of barley (Iranian cv. Nosrat)

MUTAGENIC EFFECTS OF SODIUM AZIDE ON THE GERMINATION IN RICE (Oryza sativa L. cv. INPAGO UNSOED 1)

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