Gamma ray effects on trophozoites of Entamoeba histolytica

Radiosensitivity of Entamoeba histolytica cysts

“…The source of gamma rays used in t'hese experiments has been described in another paper (Schneider and Porter, 1960). NRS amebae were maintained routinely in Ringer-egg-Locke (REL) slants, transferring every 48 hours.…”

“…The data from these experiments have indicated that the cysts of E. histolytica were much more sensitive to gamma rays than the trophozoites. However, the work with trophozoites (Schneider and Porter, 1960) was performed with a different strain and at a different time. Thus, it seemed advisable to compare the reactions of cysts and trophozoites of t,he same strain by irradiating them together.…”

“…In a previous study (Schneider and Porter, 1960), the radiosensitivity of a non-encysting strain (UC) of Entamoeba histolytica was reported. Subcultures of irradiated trophozoites began t'o display a notable increase in lag phase following exposure t.o approximately 100,000 RAD (corresponding to 100 ergs per gram) of pure gamma rays.…”