Gamma radiation, in vitro selection for salt (NaCl) tolerance, and characterization of mutants in sugarcane (Saccharum officinarum L.)

Nikam, Ashok A.; Devarumath, R. M.; Shitole, M. G.; Ghole, Vikram S.; Tawar, P. N.; Suprasanna, Penna

doi:10.1007/s11627-014-9630-4

Cited by 33 publications

(26 citation statements)

References 35 publications

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“…Both abiotic and biotic tolerant plants could be obtained from the media which contain the selection agent [16]. In vitro selection has been practiced for desirable traits and success has been achieved in several crop plants [18][19][20]. Easy application of in vitro selection method is one of the most important requirements for the achievement of in vitro selection technique and to obtain the tolerant plants.…”

Section: Application Of Gamma Rays To Induce Mutation and In Vitro Sementioning

confidence: 99%

The Use of Gamma Irradiation in Plant Mutation Breeding

Beyaz¹,

Yıldız²

2017

Plant Engineering

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In plant breeding programs, one of the oldest methods is mutation breeding. Currently, mutation breeding has became popular among the breeders and scientists again with its use in plant biotechnology and due to some restrictions on the other techniques such as hybridization, cross breeding, and transgenic plants. Physical mutagens (X-rays, UV light, neutrons-alpha-beta particles, fast and thermal neutrons, especially gamma rays) are used more widely than chemical (ethyl methanesulfonate [EMS]) ones to artificially induce mutations (mutagenesis). However, among the physical mutagens, gamma-rays are widely used. During the irradiation of the seeds with ionizing radiation to generate mutants with desirable traits, reactive oxygen species (ROS) or free radicals can generate in cells. Although, these radicals/species generally can be very dangerous for the cell compartments, they can take an important role as a signal molecule activation of genes of antioxidant enzymes and proline, which are defense systems against these radicals in plant cells. In this chapter, usability of gamma-irradiation to provide the permanent gene expression of antioxidant enzymes and proline through the production of reactive oxygen species (ROS) is discussed.

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Section: Application Of Gamma Rays To Induce Mutation and In Vitro Sementioning

confidence: 99%

The Use of Gamma Irradiation in Plant Mutation Breeding

Beyaz¹,

Yıldız²

2017

Plant Engineering

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“…In vitro mutagenesis with pingyangmycin (PYM) as the mutagen in peanut and in vitro selection for salinity tolerance led to isolation of 23 salt-tolerant M 3 progenies (Zhao et al 2013 ). In vitro mutagenesis in combination with cellular selection was employed for obtaining salt tolerance in sugarcane (Patade et al 2006 ;Nikam et al 2014 ). Embryogenic calli were irradiated with different doses of gamma rays, subcultured and exposed to inhibitory level of NaCl.…”

Section: In Vitro Mutagenesis and Mutant Selectionmentioning

confidence: 99%

“…Salt-selected plants grown to maturity exhibited better agronomic performance under control and saline conditions. The mutant clones were characterised at the biochemical level for proline, glycine betaine, Na+ and K+ content and at the fi eld level for agronomic characters and yield (Nikam et al 2014 ).…”

Section: In Vitro Mutagenesis and Mutant Selectionmentioning

confidence: 99%

Induced Mutations and Crop Improvement

Suprasanna

Mirajkar

Bhagwat

2015

Plant Biology and Biotechnology

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Genetic variation is the mainstay which plant breeders require to produce new and improved cultivars. The opportunity of obtaining novel traits exists through induction of mutations. Induced mutations have played a signifi cant role in meeting challenges related to world food and nutritional security by way of mutant germplasm enhancement and their utilisation for the development of new mutant varieties. A wide range of genetic variability has been induced by physical and chemical mutagens. In the past several decades, induced mutations have contributed immensely to the development of improved varieties in several crop plants. Cellular and molecular biology tools have led to enhanced effi ciency of induction, detection and deployment of mutations. Till date, 3,218 mutant varieties have been released worldwide. More than 60 % of offi cially released mutant varieties are from Asia with China, India and Japan topping the list. The mutant varieties developed and released in major crops have been cultivated by farmers in large areas and have resulted in increased food production, thus contributing to food security. In this chapter, various aspects of mutation induction, applications and examples of successful use of induced mutants in crop improvement programmes are presented.

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“…Gamma radiation as a physical mutagen is the most frequently used source of ionizing radiation for creating mutants during mutation breeding . Gamma radiation has been applied to crops to develop new varieties and to improve important agronomical traits, such as resistance to disease, pathogens, salinity and drought . By applying gamma radiation, mutants with high kernel weight have been induced in wheat, and mutants with increased chlorophyll and carotenoid content have been obtained in sugar beet .…”

Section: Introductionmentioning

confidence: 99%

“…9 Gamma radiation has been applied to crops to develop new varieties and to improve important agronomical traits, such as resistance to disease, pathogens, salinity and drought. 10,11 By applying gamma radiation, mutants with high kernel weight have been induced in wheat, and mutants with increased chlorophyll and carotenoid content have been obtained in sugar beet. 12,13 Single-nucleotide polymorphisms (SNPs) in OASA1 have been identified by targeting induced local lesions in the genome of rice mutants, created using gamma radiation, with altered seed storage proteins.…”

Section: Introductionmentioning

confidence: 99%