Gamma irradiation synthesis and characterization of AgNP/gelatin/PVA hydrogels for antibacterial wound dressings

Abstract

An antibacterial hydrogel wound dressing was successfully synthesized by the gamma irradiation method. A gelatin solution was mixed with a poly(vinyl alcohol) (PVA) solution of similar concentrations at different weight ratios of 100 : 0, 80 : 20, and 60 : 40 w/w, and irradiated at 30, 40, or 50 kGy. The testing of physical properties showed that the addition of PVA could improve both durability and mechanical integrity. The 60 : 40 hydrogels irradiated at 30 kGy were optimal, and chosen to add silver nitrate at 0.25, 0.50, 0.75, or 1.00 wt % (based on the solid content) to improve the antibacterial properties. After gamma irradiation, silver nanoparticles (AgNPs) were formed. The AgNP/gelatin/PVA hydrogels were characterized for physical properties, cytotoxicity, and antibacterial activity. The AgNP/gelatin/PVA hydrogels could be used as antibacterial wound dressings because they exhibited appropriate physical properties, noncytotoxicity, and could inhibit the growth of tested bacteria. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 41138.

Simple fabrication of gelatin–polyvinyl alcohol bilayer hydrogel with wound dressing and nonadhesive duality

“…Considering that the two layers were bonded only when we poured the PVA solution at the initial stage of the physical gelation of gelatin, the mixing may have occurred due to penetration of PVA solution between the pouring and storage steps. Possible mechanisms that might have occurred in this mixed region include physical entanglement with associated hydrogen bonding and van der Waals interaction 49 or interpolymer crosslinking upon the recombination of gelatin and PVA macroradicals 50 . Characterization techniques presently used do not provide a clear indication.…”

Gamma Irradiation-Assisted Synthesis of Silver Nanoparticle and Their Antimicrobial Applications: A Review

“…Colloidal Ag chitosan NPs exhibited highly antimicrobial effect against S. aureus and Corticium salmonicolor [169]. Polyvinyl alcohol/sodium alginate/nano silver (PVA/SA/Ag) composite films by in situ gamma irradiation displayed effective inhibition of Staphylococcus aureus, methicillin-resistant S. aureus [170]. Biosynthesis of Ag-NPs from Enterococcus sp.…”

Radiation Influence on Edible Materials

“…Gamma radiation and nanocrystalline cellulose was used for the reinforcement of poly(caprolactone) composites [77]. Also, the radiation synthesis of gelatin hydrogels containing Ag nanoparticles was reported [78]. Grasielli and his group synthesized nanoparticles of seroalbumin via intramolecular cross-linking using gamma rays technology [79].…”