Gamma-amino butyric acid, glutamate dehydrogenase and glutamate decarboxylase levels in phylogenetically divergent plants

Yolcu, Seher; Özdemir, Filiz; Bor, Melike

doi:10.1007/s00606-012-0730-5

Cited by 15 publications

(8 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…8 Studies on GAD are required for a better understanding of the metabolic mechanism of GABA in plants. There have been numerous studies that have investigated the regulation and activity of GAD in different plants; [20][21][22] also, the enzymatic properties of plant GAD have been studied in detail. [15][16][17] Plant GAD generally contains a C-terminal region known as the Ca 2+ /calmodulin (CaM)-binding domain.…”

Section: Discussionmentioning

confidence: 99%

“…Not surprisingly, 5.0 mmol L À1 of aminoxyacetate not only inhibited GAD activity but also suppressed the growth of fava bean sprouts during germination, since the GABA shunt has been proven to play an important role in plant carbon and nitrogen partitioning and development. 8,20 Moreover, Barbosa et al suggested that the plant GAD plays a critical role in the regulation of pH, mineral acquisition and nitrogen uptake. 27 GAD activity may also have a relationship with the earlier diversication and evolution of vascular plants.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of the inhibitor of glutamate decarboxylase on the development and GABA accumulation in germinating fava beans under hypoxia-NaCl stress

2018

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effects of the inhibitor of glutamate decarboxylase on the development and GABA accumulation in germinating fava beans under hypoxia-NaCl stress

2018

View full text Add to dashboard Cite

show abstract

“…γ-Aminobutyric acid (GABA) is a non-protein amino acid and widely exists in eukaryotes and prokaryotes [9]. As an important nerve conduction signal in animals, GABA has been broadly studied and its function is involved in metabolic regulation, antioxidation, and nutrient digestibility under stress condition [10,11].…”

Section: Introductionmentioning

confidence: 99%

Regulation of Heat Shock Factor Pathways by γ-aminobutyric Acid (GABA) Associated with Thermotolerance of Creeping Bentgrass

Liu

Zhou

et al. 2019

IJMS

View full text Add to dashboard Cite

Activation and enhancement of heat shock factor (HSF) pathways are important adaptive responses to heat stress in plants. The γ-aminobutyric acid (GABA) plays an important role in regulating heat tolerance, but it is unclear whether GABA-induced thermotolerance is associated with activation of HSF pathways in plants. In this study, the changes of endogenous GABA level affecting physiological responses and genes involved in HSF pathways were investigated in creeping bentgrass during heat stress. The increase in endogenous GABA content induced by exogenous application of GABA effectively alleviated heat damage, as reflected by higher leaf relative water content, cell membrane stability, photosynthesis, and lower oxidative damage. Contrarily, the inhibition of GABA accumulation by the application of GABA biosynthesis inhibitor further aggravated heat damage. Transcriptional analyses showed that exogenous GABA could significantly upregulate transcript levels of genes encoding heat shock factor HSFs (HSFA-6a, HSFA-2c, and HSFB-2b), heat shock proteins (HSP17.8, HSP26.7, HSP70, and HSP90.1-b1), and ascorbate peroxidase 3 (APX3), whereas the inhibition of GABA biosynthesis depressed these genes expression under heat stress. Our results indicate GABA regulates thermotolerance associated with activation and enhancement of HSF pathways in creeping bentgrass.

show abstract

“…GABA can be produced by many kinds of organisms such as plants and fungi (Trichoderma atroviride, Aspergillus oryzae and Mo nascus sp.) (30)(31)(32). The upregulated proteins play various roles, especially in secondary metabolite production.…”

Section: The Diff Erential Protein Expression Of Mycelia At Diff Erenmentioning

confidence: 99%

Effect of Temperature-Shift and Temperature-Constant Cultivation on the Monacolin K Biosynthetic Gene Cluster Expression in Monascus

Lin

Wang

et al. 2017

Food Technol. Biotechnol.

View full text Add to dashboard Cite

SummaryIn this study, the eff ects of temperature-shift (from 30 to 25 °C) and temperature-constant (at 30 °C) cultivation on the mass of Monascus fuliginosus CG-6 mycelia and concentration of the produced monacolin K (MK) were monitored. The expression levels of the MK biosynthetic genes of M. fuliginosus CG-6 at constant and variable culture temperatures were analysed by real-time quantitative polymerase chain reaction (RT-qPCR). The total protein was collected and determined by liquid chromatography-electrospray ionisation with tandem mass spectrometry (LC-ESI-MS/MS). Results showed that the maximum mycelial mass in temperature-shift cultivation was only 0.477 g of dry cell mass per dish, which was lower than that in temperature-constant cultivation (0.581 g of dry cell mass per dish); however, the maximum concentration of MK in temperature-shift cultivation (34.5 μg/mL) was 16 times higher than that in temperature-constant cultivation at 30 °C (2.11 μg/mL). Gene expression analysis showed that the expression of the MK biosynthetic gene cluster at culture temperature of 25 °C was higher than that at 30 °C, which was similar to the trend of the MK concentration, except for individual MK B and MK C genes. Analysis of diff erential protein exp ression revealed that 2016 proteins were detected by LC-ESI-MS/MS. The expression level of effl ux pump protein coded by the MK I gene exhibited the same upregulated trend as the expression of MK I in temperature-shift cultivation. Temperature-shift cultivation enhanced the expression of proteins in the secondary metabolite production pathway, but suppressed the expression of proteins involved in the mycelial growth.

show abstract

Gamma-amino butyric acid, glutamate dehydrogenase and glutamate decarboxylase levels in phylogenetically divergent plants

Cited by 15 publications

References 46 publications

Effects of the inhibitor of glutamate decarboxylase on the development and GABA accumulation in germinating fava beans under hypoxia-NaCl stress

Effects of the inhibitor of glutamate decarboxylase on the development and GABA accumulation in germinating fava beans under hypoxia-NaCl stress

Regulation of Heat Shock Factor Pathways by γ-aminobutyric Acid (GABA) Associated with Thermotolerance of Creeping Bentgrass

Effect of Temperature-Shift and Temperature-Constant Cultivation on the Monacolin K Biosynthetic Gene Cluster Expression in Monascus

Contact Info

Product

Resources

About