Gamma Amino Butyric Acid Ameliorates Jejunal Oxidative Damage in Diabetic Rats

Abstract

Introduction:The small intestine exhibits morphological and functional changes in streptozotocin (STZ)-induced diabetes. This study was carried out to clarify the role of gamma-aminobutyric acid (GABA) in ameliorating the histopathological changes and the oxidative stress in small intestine of the diabetic rats. Material and Methods: For this purpose, 40 adult male Swiss albino rats (Sprague dawley strain) were divided into 4 groups (10 animals in each group); the control group, GABA treated group, the diabetic group and the fourth group was subjected to induction of diabetes and GABA treatment. All animals were sacrificed and specimens from jejunum were removed and processed for the histopathological examinations, cytomorphometric analysis and oxidative stress assessment. Results: In diabetic rats, Jejunal sections from the diabetic rats exhibited pleomorphism of villi, inflammatory cellular infiltration and edema in the villous core with lymphoid hyperplasia, an increase in goblet cells number was also observed with disappearance of Paneth cells or they were degranulated, increased crypt numbers and mucosal height and increased thickness of muscularis layer. Moreover, there was an increase in the oxidant biomarkers with decline in the antioxidant enzymes in Jejunal sections of the diabetic rats. Conclusion: Treatment of rats with GABA ameliorated the observed histopathological alterations, morphometric changes and improved the oxidant/ antioxidant statusof the jejunum in diabetic rats. Based on these results, it was concluded that GABA administration to rats ameliorated diabetes-induced jejunal histopathological and biochemical alterations.

Gamma-aminobutyric acid ameliorates gamma rays-induced oxidative stress in the small intestine of rats

“…Glutamate, the raw material for glutathione synthesis in the antioxidation system, recover and maintain the reduced glutathione level, by this way improving the activity of GSH-Px in the antioxidation system (Chen, Tang, Sun, & Xie, 2013). Moreover, the modulator role of GABA following radiation exposure may also be attributed to its antioxidant and free radical-scavenging activities (Deng et al, 2013;Eltahawy, Abd El-Hady, Badawi, & Hammad, 2017). Thus, GABA can attenuate various morphological, histopathological, and biochemical changes resulting from intestinal injury following radiation exposure demonstrating that GABA provides a significant ameliorative effect against intestinal injury following irradiation process.…”

The Effect of Induced Diabetes Mellitus on the Cerebellar Cortex of Adult Male Rat and the Possible Protective Role of Oxymatrine: A Histological, Immunohistochemical and Biochemical Study

“…72 hours after STZ injection, diabetes mellitus induction was con rmed by evaluation of the glucose levels in blood samples obtained from the tail vein using the ACCU-CHEK glucose meter (Roche Diagnostic Corporation, Indianapolis, IN). Only animals with blood glucose level of (≥ 250mg/dl) were considered diabetic (Eltahawy et al, 2017). In this research, only 22 rats were proved to develop DM after STZ injection and were further subdivided into 2 experimental subgroups: Subgroup IIIa (Induced diabetic group): included 11 rats that received no additional treatment.…”

The Effect of Polyphenol Extract from Rosa Roxburghii Fruit on Plasma Metabolome and Gut Microbiota in Type 2 Diabetic Mice

“…The results were consistent with previous studies, which have reported that the intake of polyphenol extracts from purple sweet potato [ 19 ] and black goji berry [ 20 ] have improved the inflammatory response in diabetes. Gut inflammation and oxidative stress have been identified in diabetes [ 21 , 22 , 23 ]. Oxidative stress has been considered one of the risk factors in the development of diabetes [ 24 ].…”