Gamete and Embryo Manipulation

Rosen, Mitchell P.; Yang, Xing; Marsh, Philip; Runge, Andrew; Olivera, Giovanna; Ribeiro, Salustiano; Simbulan, Rhodel; Quinn, Molly M.

doi:10.1016/b978-0-323-47912-7.00032-9

Cited by 4 publications

(3 citation statements)

References 609 publications

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“…SF, rapid freezing, and vitrification are sperm cryopreservation methods, discovered during the performance of several cryo-experimental improvements ( 81 , 82 ). SF was developed by Behrman and Sawada and includes progressive sperm cooling in two or three steps, either manually or automatically ( 37 , 83 ).…”

Section: Routinely Applied Assisted Reproductive Technologies For Fer...mentioning

confidence: 99%

“…In comparison with other FP procedures, the OTC process is of increased complexity, owing to the presence of multiple and diverse cell populations. It is known that the presence of these cells may negatively affect the permeation of cryoprotectants, thus also affecting the survival of the follicles ( 81 ). In contrast to oocyte vitrification, OTC showed a decreased LBRs trend, but still, today remains the only promising choice for some of the aforenamed patients ( 124 ).…”

Section: Not Routinely Applied Assisted Reproductive Technologies For...mentioning

confidence: 99%

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A comprehensive review and update on human fertility cryopreservation methods and tools

et al. 2023

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The broad conceptualization of fertility preservation and restoration has become already a major concern in the modern western world since a large number of individuals often face it in the everyday life. Driven by different health conditions and/or social reasons, a variety of patients currently rely on routinely and non-routinely applied assisted reproductive technologies, and mostly on the possibility to cryopreserve gametes and/or gonadal tissues for expanding their reproductive lifespan. This review embraces the data present in human-focused literature regarding the up-to-date methodologies and tools contemporarily applied in IVF laboratories' clinical setting of the oocyte, sperm, and embryo cryopreservation and explores the latest news and issues related to the optimization of methods used in ovarian and testicular tissue cryopreservation.

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Section: Routinely Applied Assisted Reproductive Technologies For Fer...mentioning

confidence: 99%

Section: Not Routinely Applied Assisted Reproductive Technologies For...mentioning

confidence: 99%

A comprehensive review and update on human fertility cryopreservation methods and tools

et al. 2023

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“…[14]). A comprehensive review of current protocols applied for embryo-assisted hatching can be found elsewhere [15], while a detailed description of all procedures performed in fertility clinics is presented in [16,17].…”

Section: Introductionmentioning

confidence: 99%

Optimal Exposure Parameters for Microsurgery of Embryo Zona Pellucida Using Femtosecond Laser Pulses

Sitnikov,

Filatov,

Ilina

2023

Applied Sciences

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We applied femtosecond laser pulses for microsurgery of the zona pellucida (ZP) of mouse embryos in terms of assisted reproductive technologies. The embryos were subjected to a series of laser pulses (wavelength of 514 nm, pulse duration of 280 fs, repetition rate of 2.5 kHz). Optical microscopy was used to study the dependence of the ZP cut width on the pulse energy E and velocity v of the laser beam. It is shown that the same value of the cut width can be obtained for different combinations of these parameters. The boundaries of admissible values were found to be E = (19–52) nJ, v = (0.001–0.03) mm/s; recommendations on their proper choice are given. An analytical expression of the cut width of the ZP for a given combination of laser-pulse energy, beam velocity, and pulse repetition rate is proposed. This simple and easy-to-use equation allows for quick prediction of ZP cut width in embryo microsurgery procedures of laser-assisted hatching and embryo laser tagging using femtosecond laser pulses.

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Leukocytospermia does not negatively impact outcomes in in vitro fertilization cycles with intracytoplasmic sperm injection and preimplantation genetic testing for aneuploidy: findings from 5435 cycles

Gill,

Puchalt,

Molinaro

et al. 2024

J Assist Reprod Genet

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Purpose To investigate whether leukocytospermia (defined as the presence of ≥ 1 × 106 white blood cells/mL) affects clinical and embryologic outcomes in in vitro fertilization (IVF) cycles with intracytoplasmic sperm injection (ICSI) and preimplantation genetic testing for aneuploidy (PGT-A). Methods This was a retrospective cohort study including 5425 cycles between January 2012 to December 2021 at a single large university-affiliated fertility clinic. The primary outcome was live birth rate (LBR). Results The prevalence of leukocytospermia was 33.9% (n = 1843). Baseline characteristics including female age, BMI, AMH, Day 3 FSH, and male partner’s age were similar in cycles with and without leukocytospermia. The LBR after the first euploid embryo transfer was similar in those with and without leukocytospermia (62.3% vs. 63% p = 0.625). Secondary outcomes including clinical pregnancy rate (CPR), sustained implantation rate (SIR), fertilization (2PN) rate, blastulation rate, and aneuploidy rate were also evaluated. The CPR (73.3% vs 74.9%, p = 0.213) and SIR (64.6% vs. 66%, p = 0.305) were similar in both groups. The 2PN rate was also similar in both groups (85.7% vs. 85.8%, p = 0.791), as was the blastulation rate per 2PN (56.7% vs. 57.5%, p = 0.116). The aneuploidy rate was not significantly different between groups (25.7% vs 24.4%, p = 0.053). A generalized estimation equation with logistic regression demonstrated that the presence leukocytospermia did not influence the LBR (adjusted OR 0.878; 95% CI, 0.680–1.138). Conclusion Leukocytospermia diagnosed just prior to an IVF cycle with PGT-A does not negatively impact clinical or embryologic outcomes.

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Gamete and Embryo Manipulation

Cited by 4 publications

References 609 publications

A comprehensive review and update on human fertility cryopreservation methods and tools

A comprehensive review and update on human fertility cryopreservation methods and tools

Optimal Exposure Parameters for Microsurgery of Embryo Zona Pellucida Using Femtosecond Laser Pulses

Leukocytospermia does not negatively impact outcomes in in vitro fertilization cycles with intracytoplasmic sperm injection and preimplantation genetic testing for aneuploidy: findings from 5435 cycles

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