GalNAc-T15 in gastric adenocarcinoma: Characterization according to tissue architecture and cellular location

Albuquerque, Mário S. de Souza; Silva-filho, Antônio F. da; Cordeiro, Marina Ferraz; Souza, Maria de Fátima Deodato de; Quirino, Michael Williames; Lima, Luiza Rayanna Amorim de; Pitta, Maíra Galdino da Rocha; Martins, Mário Rino; Rêgo, Moacyr Jesus Barreto de Melo

doi:10.4081/ejh.2018.2931

Cited by 8 publications

(5 citation statements)

References 18 publications

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“…Semi-quantitative analysis of the stained cells was done using immunoreactive score (IRS) classification by analyzing 5 random fields in each slide. 13 , 14 IRS = SI (staining intensity) x PP (percentage of positive cells). Staining intensity was determined as 0 is negative; 1, weak; 2, moderate; and 3, strong.…”

Section: Methodsmentioning

confidence: 99%

Immunopositivity for Siglec-15 in gastric cancer and its association with clinical and pathological parameters

et al. 2021

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The sialic acid-binding immunoglobulin-type lectin Siglec-15 is a promising target to cancer immunotherapy in several tumor types. The present study aimed to investigate Siglec-15 expression in gastric cancer (GC) patient tissue and to evaluate its clinical value. Siglec-15 expression was evaluated by immunohistochemistry with 71 patients. Siglec-15 staining was observed in tumor cells of 53 (74.64%) patients, with significant association with histologic classification and angiolymphatic invasion (p<0.05). Immunohistochemistry analysis also detected Siglec-15 in tumor-associated stroma cells (macrophages/myeloid cells). There was no significant association with outcomes parameters. Siglec-15 expression in well differentiated histological GC tissues and in the tumor microenvironment are potential targets to be further investigated as a novel prognostic factor for GC.

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Section: Methodsmentioning

confidence: 99%

Immunopositivity for Siglec-15 in gastric cancer and its association with clinical and pathological parameters

et al. 2021

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“…To evaluate of ST3Gal3 and Neu3 expression we followed the methods described for de Souza et al . 42 Briefly, biopsy slices were deparaffinized with xylol and rehydrated in graded ethanol. Antigen retrieval was done using citrate buffer in microwave for 15 min.…”

Section: Methodsmentioning

confidence: 99%

alpha2,3 sialic acid processing enzymes expression in gastric cancer tissues reveals that ST3Gal3 but not Neu3 are associated with Lauren's classification, angiolymphatic invasion and histological grade

et al. 2022

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Gastric cancer (GC) is one of the leading causes of cancer-related deaths worldwide. Despite progress in the last decades, there are still no reliable biomarkers for the diagnosis of and prognosis for GC. Aberrant sialylation is a widespread critical event in the development of GC. Neuraminidases (Neu) and sialyltransferases (STs) regulate the ablation and addition of sialic acid during glycoconjugates biosynthesis, and they are a considerable source of biomarkers in various cancers. This study retrospectively characterized Neu3 and ST3Gal3 expression by immunohistochemistry in 71 paraffin-embedded GC tissue specimens and analyzed the relationship between their expression and the clinicopathological parameters. Neu3 expression was markedly increased in GC tissues compared with non-tumoral tissues (p<0.0001). Intratumoral ST3Gal3 staining was significantly associated with intestinal subtype (p=0.0042) and was negatively associated with angiolymphatic invasion (p=0.0002) and higher histological grade G3 (p=0.0066). Multivariate analysis revealed that ST3Gal3 positivity is able to predict Lauren's classification. No associations were found between Neu3 staining and clinical parameters. The in silico analysis of mRNA expression in GC validation cohorts corroborates the significant ST3Gal3 association with higher histological grade observed in our study. These findings suggest that ST3Gal3 expression may be an indicator for aggressiveness of primary GC.

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“…Following the methods of Albuquerque et al (2018) [15], biopsy slices were deparaffinized with xylol and rehydrated in graded ethanol. Antigen retrieval was done using citrate buffer in a microwave.…”

Section: Methodsmentioning

confidence: 99%

Overexpression of UDP-Glucose 4-Epimerase Is Associated with Differentiation Grade of Gastric Cancer

et al. 2019

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The UDP-glucose 4-epimerase (GALE) is a glycosyltransferase, which acts on protein and lipid glycosylation in normal and neoplastic cells. This study is aimed at investigating the differential tissue expression of GALE and its possible association with clinical-pathological parameters and the outcome of gastric adenocarcinoma patients. Seventy-one patients were evaluated in relation to GALE expression by immunohistochemistry. Our results showed that 48 (67.6%) patients were GALE positive and 23 (32.4%) negative. Positive staining was present on well-differentiated and moderate-differentiated histological grade of gastric adenocarcinomas (p < 0.0001). There was no significant association with outcome parameters (p > 0.05). Besides that, our results corroborated with the validation cohort analysis, where the expression of GALE mRNA was also associated with the histological grade (p < 0.001). These results suggest a possible use of this enzyme as a biomarker for well and moderately differentiated tumors.

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GalNAc-T15 in gastric adenocarcinoma: Characterization according to tissue architecture and cellular location

Cited by 8 publications

References 18 publications

Immunopositivity for Siglec-15 in gastric cancer and its association with clinical and pathological parameters

Immunopositivity for Siglec-15 in gastric cancer and its association with clinical and pathological parameters

alpha2,3 sialic acid processing enzymes expression in gastric cancer tissues reveals that ST3Gal3 but not Neu3 are associated with Lauren's classification, angiolymphatic invasion and histological grade

Overexpression of UDP-Glucose 4-Epimerase Is Associated with Differentiation Grade of Gastric Cancer

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